Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<

The predominantly vegetative propagating duckweeds are of growing commercial interest. Since clonal accessions within a respective species can vary considerably with respect to their physiological as well as biochemical traits, it is critical to be able to track the clones of species of interest aft...
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Autor*in:	Manuela Bog [verfasserIn] Luca Braglia [verfasserIn] Laura Morello [verfasserIn] Karen I. Noboa Melo [verfasserIn] Ingo Schubert [verfasserIn] Oleg N. Shchepin [verfasserIn] K. Sowjanya Sree [verfasserIn] Shuqing Xu [verfasserIn] Eric Lam [verfasserIn] Klaus J. Appenroth [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Lemnaceae duckweed genotyping intraspecific variation

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 11(2022), 22, p 3033
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:22, p 3033

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants11223033

Katalog-ID:	DOAJ017834856

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authorswithroles_txt_mv	Manuela Bog @@aut@@ Luca Braglia @@aut@@ Laura Morello @@aut@@ Karen I. Noboa Melo @@aut@@ Ingo Schubert @@aut@@ Oleg N. Shchepin @@aut@@ K. Sowjanya Sree @@aut@@ Shuqing Xu @@aut@@ Eric Lam @@aut@@ Klaus J. Appenroth @@aut@@
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callnumber-first	Q - Science
author	Manuela Bog
spellingShingle	Manuela Bog misc QK1-989 misc <i<Spirodela polyrhiza</i< misc Lemnaceae misc duckweed misc genotyping misc intraspecific variation misc Botany Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<
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topic_title	QK1-989 Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i< <i<Spirodela polyrhiza</i< Lemnaceae duckweed genotyping intraspecific variation
topic	misc QK1-989 misc <i<Spirodela polyrhiza</i< misc Lemnaceae misc duckweed misc genotyping misc intraspecific variation misc Botany
topic_unstemmed	misc QK1-989 misc <i<Spirodela polyrhiza</i< misc Lemnaceae misc duckweed misc genotyping misc intraspecific variation misc Botany
topic_browse	misc QK1-989 misc <i<Spirodela polyrhiza</i< misc Lemnaceae misc duckweed misc genotyping misc intraspecific variation misc Botany
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title	Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<
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title_full	Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<
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author_browse	Manuela Bog Luca Braglia Laura Morello Karen I. Noboa Melo Ingo Schubert Oleg N. Shchepin K. Sowjanya Sree Shuqing Xu Eric Lam Klaus J. Appenroth
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title_sort	strategies for intraspecific genotyping of duckweed: comparison of five orthogonal methods applied to the giant duckweed <i<spirodela polyrhiza</i<
callnumber	QK1-989
title_auth	Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<
abstract	The predominantly vegetative propagating duckweeds are of growing commercial interest. Since clonal accessions within a respective species can vary considerably with respect to their physiological as well as biochemical traits, it is critical to be able to track the clones of species of interest after their characterization. Here, we compared the efficacy of five different genotyping methods for <i<Spirodela polyrhiza</i<, a species with very low intraspecific sequence variations, including polymorphic NB-ARC-related loci, tubulin-gene-based polymorphism (TBP), simple sequence repeat variations (SSR), multiplexed ISSR genotyping by sequencing (MIG-seq), and low-coverage, reduced-representation genome sequencing (GBS). Four of the five approaches could distinguish 20 to 22 genotypes out of the 23 investigated clones, while TBP resolved just seven genotypes. The choice for a particular method for intraspecific genotyping can depend on the research question and the project budget, while the combination of orthogonal methods may increase the confidence and resolution for the results obtained.
abstractGer	The predominantly vegetative propagating duckweeds are of growing commercial interest. Since clonal accessions within a respective species can vary considerably with respect to their physiological as well as biochemical traits, it is critical to be able to track the clones of species of interest after their characterization. Here, we compared the efficacy of five different genotyping methods for <i<Spirodela polyrhiza</i<, a species with very low intraspecific sequence variations, including polymorphic NB-ARC-related loci, tubulin-gene-based polymorphism (TBP), simple sequence repeat variations (SSR), multiplexed ISSR genotyping by sequencing (MIG-seq), and low-coverage, reduced-representation genome sequencing (GBS). Four of the five approaches could distinguish 20 to 22 genotypes out of the 23 investigated clones, while TBP resolved just seven genotypes. The choice for a particular method for intraspecific genotyping can depend on the research question and the project budget, while the combination of orthogonal methods may increase the confidence and resolution for the results obtained.
abstract_unstemmed	The predominantly vegetative propagating duckweeds are of growing commercial interest. Since clonal accessions within a respective species can vary considerably with respect to their physiological as well as biochemical traits, it is critical to be able to track the clones of species of interest after their characterization. Here, we compared the efficacy of five different genotyping methods for <i<Spirodela polyrhiza</i<, a species with very low intraspecific sequence variations, including polymorphic NB-ARC-related loci, tubulin-gene-based polymorphism (TBP), simple sequence repeat variations (SSR), multiplexed ISSR genotyping by sequencing (MIG-seq), and low-coverage, reduced-representation genome sequencing (GBS). Four of the five approaches could distinguish 20 to 22 genotypes out of the 23 investigated clones, while TBP resolved just seven genotypes. The choice for a particular method for intraspecific genotyping can depend on the research question and the project budget, while the combination of orthogonal methods may increase the confidence and resolution for the results obtained.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	22, p 3033
title_short	Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<
url	https://doi.org/10.3390/plants11223033 https://doaj.org/article/4fdf2f459ef54dca8b9574d8a2202fbb https://www.mdpi.com/2223-7747/11/22/3033 https://doaj.org/toc/2223-7747
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author2	Luca Braglia Laura Morello Karen I. Noboa Melo Ingo Schubert Oleg N. Shchepin K. Sowjanya Sree Shuqing Xu Eric Lam Klaus J. Appenroth
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up_date	2024-07-03T14:22:44.550Z
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Strategies for Intraspecific Genotyping of Duckweed: Comparison of Five Orthogonal Methods Applied to the Giant Duckweed <i<Spirodela polyrhiza</i<

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