Single molecule characterization of individual extracellular vesicles from pancreatic cancer

Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensive...
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Autor*in:	Kathleen M. Lennon [verfasserIn] Devin L. Wakefield [verfasserIn] Adam L. Maddox [verfasserIn] Matthew S. Brehove [verfasserIn] Ari N. Willner [verfasserIn] Krystine Garcia-Mansfield [verfasserIn] Bessie Meechoovet [verfasserIn] Rebecca Reiman [verfasserIn] Elizabeth Hutchins [verfasserIn] Marcia M. Miller [verfasserIn] Ajay Goel [verfasserIn] Patrick Pirrotte [verfasserIn] Kendall Van Keuren-Jensen [verfasserIn] Tijana Jovanovic-Talisman [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics

Übergeordnetes Werk:	In: Journal of Extracellular Vesicles - Wiley, 2012, 8(2019), 1
Übergeordnetes Werk:	volume:8 ; year:2019 ; number:1

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1080/20013078.2019.1685634

Katalog-ID:	DOAJ018056229

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520			\|a Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.
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650		4	\|a translational medicine
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653		0	\|a Cytology
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700	0		\|a Adam L. Maddox \|e verfasserin \|4 aut
700	0		\|a Matthew S. Brehove \|e verfasserin \|4 aut
700	0		\|a Ari N. Willner \|e verfasserin \|4 aut
700	0		\|a Krystine Garcia-Mansfield \|e verfasserin \|4 aut
700	0		\|a Bessie Meechoovet \|e verfasserin \|4 aut
700	0		\|a Rebecca Reiman \|e verfasserin \|4 aut
700	0		\|a Elizabeth Hutchins \|e verfasserin \|4 aut
700	0		\|a Marcia M. Miller \|e verfasserin \|4 aut
700	0		\|a Ajay Goel \|e verfasserin \|4 aut
700	0		\|a Patrick Pirrotte \|e verfasserin \|4 aut
700	0		\|a Kendall Van Keuren-Jensen \|e verfasserin \|4 aut
700	0		\|a Tijana Jovanovic-Talisman \|e verfasserin \|4 aut
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allfields	10.1080/20013078.2019.1685634 doi (DE-627)DOAJ018056229 (DE-599)DOAJ364c6031b13846c3a8651fb570b8f5c3 DE-627 ger DE-627 rakwb eng QH573-671 Kathleen M. Lennon verfasserin aut Single molecule characterization of individual extracellular vesicles from pancreatic cancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy. quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology Devin L. Wakefield verfasserin aut Adam L. Maddox verfasserin aut Matthew S. Brehove verfasserin aut Ari N. Willner verfasserin aut Krystine Garcia-Mansfield verfasserin aut Bessie Meechoovet verfasserin aut Rebecca Reiman verfasserin aut Elizabeth Hutchins verfasserin aut Marcia M. Miller verfasserin aut Ajay Goel verfasserin aut Patrick Pirrotte verfasserin aut Kendall Van Keuren-Jensen verfasserin aut Tijana Jovanovic-Talisman verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 8(2019), 1 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:8 year:2019 number:1 https://doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 kostenfrei http://dx.doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 1
spelling	10.1080/20013078.2019.1685634 doi (DE-627)DOAJ018056229 (DE-599)DOAJ364c6031b13846c3a8651fb570b8f5c3 DE-627 ger DE-627 rakwb eng QH573-671 Kathleen M. Lennon verfasserin aut Single molecule characterization of individual extracellular vesicles from pancreatic cancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy. quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology Devin L. Wakefield verfasserin aut Adam L. Maddox verfasserin aut Matthew S. Brehove verfasserin aut Ari N. Willner verfasserin aut Krystine Garcia-Mansfield verfasserin aut Bessie Meechoovet verfasserin aut Rebecca Reiman verfasserin aut Elizabeth Hutchins verfasserin aut Marcia M. Miller verfasserin aut Ajay Goel verfasserin aut Patrick Pirrotte verfasserin aut Kendall Van Keuren-Jensen verfasserin aut Tijana Jovanovic-Talisman verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 8(2019), 1 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:8 year:2019 number:1 https://doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 kostenfrei http://dx.doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 1
allfields_unstemmed	10.1080/20013078.2019.1685634 doi (DE-627)DOAJ018056229 (DE-599)DOAJ364c6031b13846c3a8651fb570b8f5c3 DE-627 ger DE-627 rakwb eng QH573-671 Kathleen M. Lennon verfasserin aut Single molecule characterization of individual extracellular vesicles from pancreatic cancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy. quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology Devin L. Wakefield verfasserin aut Adam L. Maddox verfasserin aut Matthew S. Brehove verfasserin aut Ari N. Willner verfasserin aut Krystine Garcia-Mansfield verfasserin aut Bessie Meechoovet verfasserin aut Rebecca Reiman verfasserin aut Elizabeth Hutchins verfasserin aut Marcia M. Miller verfasserin aut Ajay Goel verfasserin aut Patrick Pirrotte verfasserin aut Kendall Van Keuren-Jensen verfasserin aut Tijana Jovanovic-Talisman verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 8(2019), 1 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:8 year:2019 number:1 https://doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 kostenfrei http://dx.doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 1
allfieldsGer	10.1080/20013078.2019.1685634 doi (DE-627)DOAJ018056229 (DE-599)DOAJ364c6031b13846c3a8651fb570b8f5c3 DE-627 ger DE-627 rakwb eng QH573-671 Kathleen M. Lennon verfasserin aut Single molecule characterization of individual extracellular vesicles from pancreatic cancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy. quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology Devin L. Wakefield verfasserin aut Adam L. Maddox verfasserin aut Matthew S. Brehove verfasserin aut Ari N. Willner verfasserin aut Krystine Garcia-Mansfield verfasserin aut Bessie Meechoovet verfasserin aut Rebecca Reiman verfasserin aut Elizabeth Hutchins verfasserin aut Marcia M. Miller verfasserin aut Ajay Goel verfasserin aut Patrick Pirrotte verfasserin aut Kendall Van Keuren-Jensen verfasserin aut Tijana Jovanovic-Talisman verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 8(2019), 1 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:8 year:2019 number:1 https://doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 kostenfrei http://dx.doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 1
allfieldsSound	10.1080/20013078.2019.1685634 doi (DE-627)DOAJ018056229 (DE-599)DOAJ364c6031b13846c3a8651fb570b8f5c3 DE-627 ger DE-627 rakwb eng QH573-671 Kathleen M. Lennon verfasserin aut Single molecule characterization of individual extracellular vesicles from pancreatic cancer 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy. quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology Devin L. Wakefield verfasserin aut Adam L. Maddox verfasserin aut Matthew S. Brehove verfasserin aut Ari N. Willner verfasserin aut Krystine Garcia-Mansfield verfasserin aut Bessie Meechoovet verfasserin aut Rebecca Reiman verfasserin aut Elizabeth Hutchins verfasserin aut Marcia M. Miller verfasserin aut Ajay Goel verfasserin aut Patrick Pirrotte verfasserin aut Kendall Van Keuren-Jensen verfasserin aut Tijana Jovanovic-Talisman verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 8(2019), 1 (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:8 year:2019 number:1 https://doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 kostenfrei http://dx.doi.org/10.1080/20013078.2019.1685634 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 1
language	English
source	In Journal of Extracellular Vesicles 8(2019), 1 volume:8 year:2019 number:1
sourceStr	In Journal of Extracellular Vesicles 8(2019), 1 volume:8 year:2019 number:1
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics Cytology
isfreeaccess_bool	true
container_title	Journal of Extracellular Vesicles
authorswithroles_txt_mv	Kathleen M. Lennon @@aut@@ Devin L. Wakefield @@aut@@ Adam L. Maddox @@aut@@ Matthew S. Brehove @@aut@@ Ari N. Willner @@aut@@ Krystine Garcia-Mansfield @@aut@@ Bessie Meechoovet @@aut@@ Rebecca Reiman @@aut@@ Elizabeth Hutchins @@aut@@ Marcia M. Miller @@aut@@ Ajay Goel @@aut@@ Patrick Pirrotte @@aut@@ Kendall Van Keuren-Jensen @@aut@@ Tijana Jovanovic-Talisman @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	726923710
id	DOAJ018056229
language_de	englisch
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callnumber-first	Q - Science
author	Kathleen M. Lennon
spellingShingle	Kathleen M. Lennon misc QH573-671 misc quantitative single molecule localization microscopy misc extracellular vesicles misc pancreatic cancer misc translational medicine misc imaging misc diagnostics misc Cytology Single molecule characterization of individual extracellular vesicles from pancreatic cancer
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topic_title	QH573-671 Single molecule characterization of individual extracellular vesicles from pancreatic cancer quantitative single molecule localization microscopy extracellular vesicles pancreatic cancer translational medicine imaging diagnostics
topic	misc QH573-671 misc quantitative single molecule localization microscopy misc extracellular vesicles misc pancreatic cancer misc translational medicine misc imaging misc diagnostics misc Cytology
topic_unstemmed	misc QH573-671 misc quantitative single molecule localization microscopy misc extracellular vesicles misc pancreatic cancer misc translational medicine misc imaging misc diagnostics misc Cytology
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title	Single molecule characterization of individual extracellular vesicles from pancreatic cancer
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title_full	Single molecule characterization of individual extracellular vesicles from pancreatic cancer
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author_browse	Kathleen M. Lennon Devin L. Wakefield Adam L. Maddox Matthew S. Brehove Ari N. Willner Krystine Garcia-Mansfield Bessie Meechoovet Rebecca Reiman Elizabeth Hutchins Marcia M. Miller Ajay Goel Patrick Pirrotte Kendall Van Keuren-Jensen Tijana Jovanovic-Talisman
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title_sort	single molecule characterization of individual extracellular vesicles from pancreatic cancer
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title_auth	Single molecule characterization of individual extracellular vesicles from pancreatic cancer
abstract	Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.
abstractGer	Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.
abstract_unstemmed	Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.
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title_short	Single molecule characterization of individual extracellular vesicles from pancreatic cancer
url	https://doi.org/10.1080/20013078.2019.1685634 https://doaj.org/article/364c6031b13846c3a8651fb570b8f5c3 http://dx.doi.org/10.1080/20013078.2019.1685634 https://doaj.org/toc/2001-3078
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author2	Devin L. Wakefield Adam L. Maddox Matthew S. Brehove Ari N. Willner Krystine Garcia-Mansfield Bessie Meechoovet Rebecca Reiman Elizabeth Hutchins Marcia M. Miller Ajay Goel Patrick Pirrotte Kendall Van Keuren-Jensen Tijana Jovanovic-Talisman
author2Str	Devin L. Wakefield Adam L. Maddox Matthew S. Brehove Ari N. Willner Krystine Garcia-Mansfield Bessie Meechoovet Rebecca Reiman Elizabeth Hutchins Marcia M. Miller Ajay Goel Patrick Pirrotte Kendall Van Keuren-Jensen Tijana Jovanovic-Talisman
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up_date	2024-07-03T15:41:23.428Z
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Lennon</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Single molecule characterization of individual extracellular vesicles from pancreatic cancer</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. 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Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">quantitative single molecule localization microscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">extracellular vesicles</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">pancreatic cancer</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">translational medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">imaging</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">diagnostics</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Cytology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Devin L. 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Willner</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Krystine Garcia-Mansfield</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Bessie Meechoovet</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Rebecca Reiman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Elizabeth Hutchins</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marcia M. 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Single molecule characterization of individual extracellular vesicles from pancreatic cancer

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