Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy

<i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in C...
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Gespeichert in:

Autor*in:	Eui-Joon Kil [verfasserIn] Thuy Thi Bich Vo [verfasserIn] Chairina Fadhila [verfasserIn] Phuong Thi Ho [verfasserIn] Aamir Lal [verfasserIn] Elisa Troiano [verfasserIn] Giuseppe Parrella [verfasserIn] Sukchan Lee [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV)

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 9(2020), 5, p 563
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:5, p 563

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants9050563

Katalog-ID:	DOAJ018103219

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allfields	10.3390/plants9050563 doi (DE-627)DOAJ018103219 (DE-599)DOAJb6f20a5f41a648909eca8e97a4de232b DE-627 ger DE-627 rakwb eng QK1-989 Eui-Joon Kil verfasserin aut Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy. begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV) Botany Thuy Thi Bich Vo verfasserin aut Chairina Fadhila verfasserin aut Phuong Thi Ho verfasserin aut Aamir Lal verfasserin aut Elisa Troiano verfasserin aut Giuseppe Parrella verfasserin aut Sukchan Lee verfasserin aut In Plants MDPI AG, 2013 9(2020), 5, p 563 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:9 year:2020 number:5, p 563 https://doi.org/10.3390/plants9050563 kostenfrei https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b kostenfrei https://www.mdpi.com/2223-7747/9/5/563 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 563
spelling	10.3390/plants9050563 doi (DE-627)DOAJ018103219 (DE-599)DOAJb6f20a5f41a648909eca8e97a4de232b DE-627 ger DE-627 rakwb eng QK1-989 Eui-Joon Kil verfasserin aut Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy. begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV) Botany Thuy Thi Bich Vo verfasserin aut Chairina Fadhila verfasserin aut Phuong Thi Ho verfasserin aut Aamir Lal verfasserin aut Elisa Troiano verfasserin aut Giuseppe Parrella verfasserin aut Sukchan Lee verfasserin aut In Plants MDPI AG, 2013 9(2020), 5, p 563 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:9 year:2020 number:5, p 563 https://doi.org/10.3390/plants9050563 kostenfrei https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b kostenfrei https://www.mdpi.com/2223-7747/9/5/563 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 563
allfields_unstemmed	10.3390/plants9050563 doi (DE-627)DOAJ018103219 (DE-599)DOAJb6f20a5f41a648909eca8e97a4de232b DE-627 ger DE-627 rakwb eng QK1-989 Eui-Joon Kil verfasserin aut Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy. begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV) Botany Thuy Thi Bich Vo verfasserin aut Chairina Fadhila verfasserin aut Phuong Thi Ho verfasserin aut Aamir Lal verfasserin aut Elisa Troiano verfasserin aut Giuseppe Parrella verfasserin aut Sukchan Lee verfasserin aut In Plants MDPI AG, 2013 9(2020), 5, p 563 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:9 year:2020 number:5, p 563 https://doi.org/10.3390/plants9050563 kostenfrei https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b kostenfrei https://www.mdpi.com/2223-7747/9/5/563 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 563
allfieldsGer	10.3390/plants9050563 doi (DE-627)DOAJ018103219 (DE-599)DOAJb6f20a5f41a648909eca8e97a4de232b DE-627 ger DE-627 rakwb eng QK1-989 Eui-Joon Kil verfasserin aut Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy. begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV) Botany Thuy Thi Bich Vo verfasserin aut Chairina Fadhila verfasserin aut Phuong Thi Ho verfasserin aut Aamir Lal verfasserin aut Elisa Troiano verfasserin aut Giuseppe Parrella verfasserin aut Sukchan Lee verfasserin aut In Plants MDPI AG, 2013 9(2020), 5, p 563 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:9 year:2020 number:5, p 563 https://doi.org/10.3390/plants9050563 kostenfrei https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b kostenfrei https://www.mdpi.com/2223-7747/9/5/563 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 563
allfieldsSound	10.3390/plants9050563 doi (DE-627)DOAJ018103219 (DE-599)DOAJb6f20a5f41a648909eca8e97a4de232b DE-627 ger DE-627 rakwb eng QK1-989 Eui-Joon Kil verfasserin aut Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy. begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV) Botany Thuy Thi Bich Vo verfasserin aut Chairina Fadhila verfasserin aut Phuong Thi Ho verfasserin aut Aamir Lal verfasserin aut Elisa Troiano verfasserin aut Giuseppe Parrella verfasserin aut Sukchan Lee verfasserin aut In Plants MDPI AG, 2013 9(2020), 5, p 563 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:9 year:2020 number:5, p 563 https://doi.org/10.3390/plants9050563 kostenfrei https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b kostenfrei https://www.mdpi.com/2223-7747/9/5/563 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 563
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authorswithroles_txt_mv	Eui-Joon Kil @@aut@@ Thuy Thi Bich Vo @@aut@@ Chairina Fadhila @@aut@@ Phuong Thi Ho @@aut@@ Aamir Lal @@aut@@ Elisa Troiano @@aut@@ Giuseppe Parrella @@aut@@ Sukchan Lee @@aut@@
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callnumber-first	Q - Science
author	Eui-Joon Kil
spellingShingle	Eui-Joon Kil misc QK1-989 misc begomovirus misc seed transmission misc tomato leaf curl New Delhi virus (ToLCNDV) misc Botany Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy
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topic_title	QK1-989 Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy begomovirus seed transmission tomato leaf curl New Delhi virus (ToLCNDV)
topic	misc QK1-989 misc begomovirus misc seed transmission misc tomato leaf curl New Delhi virus (ToLCNDV) misc Botany
topic_unstemmed	misc QK1-989 misc begomovirus misc seed transmission misc tomato leaf curl New Delhi virus (ToLCNDV) misc Botany
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title	Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy
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title_full	Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy
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author_browse	Eui-Joon Kil Thuy Thi Bich Vo Chairina Fadhila Phuong Thi Ho Aamir Lal Elisa Troiano Giuseppe Parrella Sukchan Lee
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title_sort	seed transmission of tomato leaf curl new delhi virus from zucchini squash in italy
callnumber	QK1-989
title_auth	Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy
abstract	<i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy.
abstractGer	<i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy.
abstract_unstemmed	<i<Tomato leaf curl New Delhi virus</i< (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (<i<Bemisia tabaci</i<), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy.
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title_short	Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy
url	https://doi.org/10.3390/plants9050563 https://doaj.org/article/b6f20a5f41a648909eca8e97a4de232b https://www.mdpi.com/2223-7747/9/5/563 https://doaj.org/toc/2223-7747
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Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. 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Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy

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