Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma

There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically i...
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Autor*in:	Karmele Valencia [verfasserIn] Cristina Sainz [verfasserIn] Cristina Bértolo [verfasserIn] Gabriel de Biurrun [verfasserIn] Jackeline Agorreta [verfasserIn] Arantza Azpilikueta [verfasserIn] Marta Larrayoz [verfasserIn] Graziella Bosco [verfasserIn] Carolina Zandueta [verfasserIn] Miriam Redrado [verfasserIn] Esther Redín [verfasserIn] Francisco Exposito [verfasserIn] Diego Serrano [verfasserIn] Mirari Echepare [verfasserIn] Daniel Ajona [verfasserIn] Ignacio Melero [verfasserIn] Ruben Pio [verfasserIn] Roman Thomas [verfasserIn] Alfonso Calvo [verfasserIn] Luis M. Montuenga [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	lung cancer squamous ntcu-mouse model syngeneic cell lines rnaseq immunotherapy

Übergeordnetes Werk:	In: Disease Models & Mechanisms - The Company of Biologists, 2011, 15(2022), 1
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:1

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1242/dmm.049137

Katalog-ID:	DOAJ018140424

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allfieldsSound	10.1242/dmm.049137 doi (DE-627)DOAJ018140424 (DE-599)DOAJc6029081bc0342b6bd4394720dcdfdaf DE-627 ger DE-627 rakwb eng RB1-214 Karmele Valencia verfasserin aut Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease. lung cancer squamous ntcu-mouse model syngeneic cell lines rnaseq immunotherapy Medicine R Pathology Cristina Sainz verfasserin aut Cristina Bértolo verfasserin aut Gabriel de Biurrun verfasserin aut Jackeline Agorreta verfasserin aut Arantza Azpilikueta verfasserin aut Marta Larrayoz verfasserin aut Graziella Bosco verfasserin aut Carolina Zandueta verfasserin aut Miriam Redrado verfasserin aut Esther Redín verfasserin aut Francisco Exposito verfasserin aut Diego Serrano verfasserin aut Mirari Echepare verfasserin aut Daniel Ajona verfasserin aut Ignacio Melero verfasserin aut Ruben Pio verfasserin aut Roman Thomas verfasserin aut Alfonso Calvo verfasserin aut Luis M. Montuenga verfasserin aut In Disease Models & Mechanisms The Company of Biologists, 2011 15(2022), 1 (DE-627)578531917 (DE-600)2451104-3 17548411 nnns volume:15 year:2022 number:1 https://doi.org/10.1242/dmm.049137 kostenfrei https://doaj.org/article/c6029081bc0342b6bd4394720dcdfdaf kostenfrei http://dmm.biologists.org/content/15/1/dmm049137 kostenfrei https://doaj.org/toc/1754-8403 Journal toc kostenfrei https://doaj.org/toc/1754-8411 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1
language	English
source	In Disease Models & Mechanisms 15(2022), 1 volume:15 year:2022 number:1
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institution	findex.gbv.de
topic_facet	lung cancer squamous ntcu-mouse model syngeneic cell lines rnaseq immunotherapy Medicine R Pathology
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authorswithroles_txt_mv	Karmele Valencia @@aut@@ Cristina Sainz @@aut@@ Cristina Bértolo @@aut@@ Gabriel de Biurrun @@aut@@ Jackeline Agorreta @@aut@@ Arantza Azpilikueta @@aut@@ Marta Larrayoz @@aut@@ Graziella Bosco @@aut@@ Carolina Zandueta @@aut@@ Miriam Redrado @@aut@@ Esther Redín @@aut@@ Francisco Exposito @@aut@@ Diego Serrano @@aut@@ Mirari Echepare @@aut@@ Daniel Ajona @@aut@@ Ignacio Melero @@aut@@ Ruben Pio @@aut@@ Roman Thomas @@aut@@ Alfonso Calvo @@aut@@ Luis M. Montuenga @@aut@@
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author	Karmele Valencia
spellingShingle	Karmele Valencia misc RB1-214 misc lung cancer misc squamous misc ntcu-mouse model misc syngeneic cell lines misc rnaseq misc immunotherapy misc Medicine misc R misc Pathology Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
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topic_title	RB1-214 Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma lung cancer squamous ntcu-mouse model syngeneic cell lines rnaseq immunotherapy
topic	misc RB1-214 misc lung cancer misc squamous misc ntcu-mouse model misc syngeneic cell lines misc rnaseq misc immunotherapy misc Medicine misc R misc Pathology
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title	Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
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title_full	Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
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author_browse	Karmele Valencia Cristina Sainz Cristina Bértolo Gabriel de Biurrun Jackeline Agorreta Arantza Azpilikueta Marta Larrayoz Graziella Bosco Carolina Zandueta Miriam Redrado Esther Redín Francisco Exposito Diego Serrano Mirari Echepare Daniel Ajona Ignacio Melero Ruben Pio Roman Thomas Alfonso Calvo Luis M. Montuenga
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title_sort	two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
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title_auth	Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
abstract	There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease.
abstractGer	There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease.
abstract_unstemmed	There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC). We have generated and characterized two models of phenotypically different transplantable LUSC cell lines, i.e. UN-SCC679 and UN-SCC680, derived from A/J mice that had been chemically induced with N-nitroso-tris-chloroethylurea (NTCU). Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole-exome and RNA sequencing. These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classic LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition. The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the organotropism of LUSC in humans, i.e. affinity to the brain, bones, liver and adrenal glands. In summary, we have generated valuable cell line tools for LUSC research, which recapitulates the complexity of the human disease.
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title_short	Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma
url	https://doi.org/10.1242/dmm.049137 https://doaj.org/article/c6029081bc0342b6bd4394720dcdfdaf http://dmm.biologists.org/content/15/1/dmm049137 https://doaj.org/toc/1754-8403 https://doaj.org/toc/1754-8411
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author2	Cristina Sainz Cristina Bértolo Gabriel de Biurrun Jackeline Agorreta Arantza Azpilikueta Marta Larrayoz Graziella Bosco Carolina Zandueta Miriam Redrado Esther Redín Francisco Exposito Diego Serrano Mirari Echepare Daniel Ajona Ignacio Melero Ruben Pio Roman Thomas Alfonso Calvo Luis M. Montuenga
author2Str	Cristina Sainz Cristina Bértolo Gabriel de Biurrun Jackeline Agorreta Arantza Azpilikueta Marta Larrayoz Graziella Bosco Carolina Zandueta Miriam Redrado Esther Redín Francisco Exposito Diego Serrano Mirari Echepare Daniel Ajona Ignacio Melero Ruben Pio Roman Thomas Alfonso Calvo Luis M. Montuenga
ppnlink	578531917
callnumber-subject	RB - Pathology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1242/dmm.049137
callnumber-a	RB1-214
up_date	2024-07-03T16:10:38.658Z
_version_	1803574877788045312
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Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma

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