cDNA Library Screening Using the SOS Recruitment System
The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the...
Ausführliche Beschreibung
Autor*in: |
Wendong Huang [verfasserIn] Sung-ling Wang [verfasserIn] Guillermina Lozano [verfasserIn] Benoit de Crombrugghe [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2001 |
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Übergeordnetes Werk: |
In: BioTechniques - Future Science Ltd, 2019, 30(2001), 1, Seite 94-100 |
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Übergeordnetes Werk: |
volume:30 ; year:2001 ; number:1 ; pages:94-100 |
Links: |
Link aufrufen |
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DOI / URN: |
10.2144/01301st06 |
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Katalog-ID: |
DOAJ018156452 |
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10.2144/01301st06 doi (DE-627)DOAJ018156452 (DE-599)DOAJ2fdb1868c10b417e9562e6121b51ae3a DE-627 ger DE-627 rakwb eng QH301-705.5 Wendong Huang verfasserin aut cDNA Library Screening Using the SOS Recruitment System 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. Biology (General) Sung-ling Wang verfasserin aut Guillermina Lozano verfasserin aut Benoit de Crombrugghe verfasserin aut In BioTechniques Future Science Ltd, 2019 30(2001), 1, Seite 94-100 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:30 year:2001 number:1 pages:94-100 https://doi.org/10.2144/01301st06 kostenfrei https://doaj.org/article/2fdb1868c10b417e9562e6121b51ae3a kostenfrei https://www.future-science.com/doi/10.2144/01301st06 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 30 2001 1 94-100 |
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10.2144/01301st06 doi (DE-627)DOAJ018156452 (DE-599)DOAJ2fdb1868c10b417e9562e6121b51ae3a DE-627 ger DE-627 rakwb eng QH301-705.5 Wendong Huang verfasserin aut cDNA Library Screening Using the SOS Recruitment System 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. Biology (General) Sung-ling Wang verfasserin aut Guillermina Lozano verfasserin aut Benoit de Crombrugghe verfasserin aut In BioTechniques Future Science Ltd, 2019 30(2001), 1, Seite 94-100 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:30 year:2001 number:1 pages:94-100 https://doi.org/10.2144/01301st06 kostenfrei https://doaj.org/article/2fdb1868c10b417e9562e6121b51ae3a kostenfrei https://www.future-science.com/doi/10.2144/01301st06 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 30 2001 1 94-100 |
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10.2144/01301st06 doi (DE-627)DOAJ018156452 (DE-599)DOAJ2fdb1868c10b417e9562e6121b51ae3a DE-627 ger DE-627 rakwb eng QH301-705.5 Wendong Huang verfasserin aut cDNA Library Screening Using the SOS Recruitment System 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. Biology (General) Sung-ling Wang verfasserin aut Guillermina Lozano verfasserin aut Benoit de Crombrugghe verfasserin aut In BioTechniques Future Science Ltd, 2019 30(2001), 1, Seite 94-100 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:30 year:2001 number:1 pages:94-100 https://doi.org/10.2144/01301st06 kostenfrei https://doaj.org/article/2fdb1868c10b417e9562e6121b51ae3a kostenfrei https://www.future-science.com/doi/10.2144/01301st06 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 30 2001 1 94-100 |
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10.2144/01301st06 doi (DE-627)DOAJ018156452 (DE-599)DOAJ2fdb1868c10b417e9562e6121b51ae3a DE-627 ger DE-627 rakwb eng QH301-705.5 Wendong Huang verfasserin aut cDNA Library Screening Using the SOS Recruitment System 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. Biology (General) Sung-ling Wang verfasserin aut Guillermina Lozano verfasserin aut Benoit de Crombrugghe verfasserin aut In BioTechniques Future Science Ltd, 2019 30(2001), 1, Seite 94-100 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:30 year:2001 number:1 pages:94-100 https://doi.org/10.2144/01301st06 kostenfrei https://doaj.org/article/2fdb1868c10b417e9562e6121b51ae3a kostenfrei https://www.future-science.com/doi/10.2144/01301st06 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 30 2001 1 94-100 |
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The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. |
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The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. |
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The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions. In SRS, the protein-protein interactions take place in the cytoplasm instead of the nucleus, as is the case in the conventional two-hybrid system. Although the SRS has overcome some of the disadvantages of the conventional two-hybrid system, it still has several problems and limitations. Here, we describe a new protocol for SRS library screening. A new combination of growth media to avoid the tedious step of replica plating greatly increases the number of independent colonies in a single library screening. Furthermore, we designed a pair of ras-specific primers and a one-step simple PCR to rule out the most abundant false positive, the mammalian ras cDNA, in SRS library screening. |
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|
score |
7.399618 |