New Tools for Oligonucleotide Fingerprinting

Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays...
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Autor*in:	A. Guerasimova [verfasserIn] L. Nyarsik [verfasserIn] I. Girnus [verfasserIn] M. Steinfath [verfasserIn] W. Wruck [verfasserIn] H. Griffiths [verfasserIn] R. Herwig [verfasserIn] C. Wierling [verfasserIn] J. O’Brien [verfasserIn] H. Eickhoff [verfasserIn] H. Lehrach [verfasserIn] U. Radelof [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2001

Übergeordnetes Werk:	In: BioTechniques - Future Science Ltd, 2019, 31(2001), 3, Seite 490-495
Übergeordnetes Werk:	volume:31 ; year:2001 ; number:3 ; pages:490-495

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.2144/01313st01

Katalog-ID:	DOAJ018156509

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520			\|a Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive.
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allfieldsGer	10.2144/01313st01 doi (DE-627)DOAJ018156509 (DE-599)DOAJ2b56c7f25951438e9c20ed8613cc268f DE-627 ger DE-627 rakwb eng QH301-705.5 A. Guerasimova verfasserin aut New Tools for Oligonucleotide Fingerprinting 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive. Biology (General) L. Nyarsik verfasserin aut I. Girnus verfasserin aut M. Steinfath verfasserin aut W. Wruck verfasserin aut H. Griffiths verfasserin aut R. Herwig verfasserin aut C. Wierling verfasserin aut J. O’Brien verfasserin aut H. Eickhoff verfasserin aut H. Lehrach verfasserin aut U. Radelof verfasserin aut In BioTechniques Future Science Ltd, 2019 31(2001), 3, Seite 490-495 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:31 year:2001 number:3 pages:490-495 https://doi.org/10.2144/01313st01 kostenfrei https://doaj.org/article/2b56c7f25951438e9c20ed8613cc268f kostenfrei https://www.future-science.com/doi/10.2144/01313st01 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2001 3 490-495
allfieldsSound	10.2144/01313st01 doi (DE-627)DOAJ018156509 (DE-599)DOAJ2b56c7f25951438e9c20ed8613cc268f DE-627 ger DE-627 rakwb eng QH301-705.5 A. Guerasimova verfasserin aut New Tools for Oligonucleotide Fingerprinting 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive. Biology (General) L. Nyarsik verfasserin aut I. Girnus verfasserin aut M. Steinfath verfasserin aut W. Wruck verfasserin aut H. Griffiths verfasserin aut R. Herwig verfasserin aut C. Wierling verfasserin aut J. O’Brien verfasserin aut H. Eickhoff verfasserin aut H. Lehrach verfasserin aut U. Radelof verfasserin aut In BioTechniques Future Science Ltd, 2019 31(2001), 3, Seite 490-495 (DE-627)306320746 (DE-600)1496354-1 19409818 nnns volume:31 year:2001 number:3 pages:490-495 https://doi.org/10.2144/01313st01 kostenfrei https://doaj.org/article/2b56c7f25951438e9c20ed8613cc268f kostenfrei https://www.future-science.com/doi/10.2144/01313st01 kostenfrei https://doaj.org/toc/0736-6205 Journal toc kostenfrei https://doaj.org/toc/1940-9818 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2001 3 490-495
language	English
source	In BioTechniques 31(2001), 3, Seite 490-495 volume:31 year:2001 number:3 pages:490-495
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title	New Tools for Oligonucleotide Fingerprinting
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title_full	New Tools for Oligonucleotide Fingerprinting
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title_sort	new tools for oligonucleotide fingerprinting
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title_auth	New Tools for Oligonucleotide Fingerprinting
abstract	Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive.
abstractGer	Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive.
abstract_unstemmed	Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries. This method currently relies on the sequential hybridizations of short, radioactively labeled DNA oligonucleotides to clone arrays. Here, we describe a new environment that substantially improves this technology. Fluorescently labeled peptide nucleic acid (PNA) oligonucleotides are used as hybridization probes. Hybridization results are recorded with a large-field, high-resolution laser scanner developed for this purpose. Automated image analysis allows easy handling of large numbers of hybridization images. Signal interference effects, which limit the gridding density in the radioactive approach, are strongly reduced. The sensitivity of the fluorescence detection demonstrated permits the convenient use of nylon membranes. Hybridization data quality is improved, and its generation is substantially accelerated, simplified, and less expensive.
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title_short	New Tools for Oligonucleotide Fingerprinting
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