Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan.
Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effec...
Ausführliche Beschreibung
Autor*in: |
Shajeela Iram [verfasserIn] Tabinda Khawaja [verfasserIn] Mehmood ul Hassan [verfasserIn] Muhammad Jawad Khan [verfasserIn] Tanveer Abbas [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2021 |
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Schlagwörter: |
Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. |
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Übergeordnetes Werk: |
In: International Journal of Endorsing Health Science Research - Advance Educational Institute & Research Centre, 2020, 9(2021), 4 |
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Übergeordnetes Werk: |
volume:9 ; year:2021 ; number:4 |
Links: |
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Katalog-ID: |
DOAJ018336124 |
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520 | |a Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. | ||
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(DE-627)DOAJ018336124 (DE-599)DOAJ6b05c8984fda4e7f9150d4883f46bc3e DE-627 ger DE-627 rakwb eng Shajeela Iram verfasserin aut Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. Medicine R Tabinda Khawaja verfasserin aut Mehmood ul Hassan verfasserin aut Muhammad Jawad Khan verfasserin aut Tanveer Abbas verfasserin aut In International Journal of Endorsing Health Science Research Advance Educational Institute & Research Centre, 2020 9(2021), 4 (DE-627)1737949660 23103841 nnns volume:9 year:2021 number:4 https://doaj.org/article/6b05c8984fda4e7f9150d4883f46bc3e kostenfrei http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/587 kostenfrei https://doaj.org/toc/2307-3748 Journal toc kostenfrei https://doaj.org/toc/2310-3841 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4 |
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(DE-627)DOAJ018336124 (DE-599)DOAJ6b05c8984fda4e7f9150d4883f46bc3e DE-627 ger DE-627 rakwb eng Shajeela Iram verfasserin aut Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. Medicine R Tabinda Khawaja verfasserin aut Mehmood ul Hassan verfasserin aut Muhammad Jawad Khan verfasserin aut Tanveer Abbas verfasserin aut In International Journal of Endorsing Health Science Research Advance Educational Institute & Research Centre, 2020 9(2021), 4 (DE-627)1737949660 23103841 nnns volume:9 year:2021 number:4 https://doaj.org/article/6b05c8984fda4e7f9150d4883f46bc3e kostenfrei http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/587 kostenfrei https://doaj.org/toc/2307-3748 Journal toc kostenfrei https://doaj.org/toc/2310-3841 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4 |
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(DE-627)DOAJ018336124 (DE-599)DOAJ6b05c8984fda4e7f9150d4883f46bc3e DE-627 ger DE-627 rakwb eng Shajeela Iram verfasserin aut Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. Medicine R Tabinda Khawaja verfasserin aut Mehmood ul Hassan verfasserin aut Muhammad Jawad Khan verfasserin aut Tanveer Abbas verfasserin aut In International Journal of Endorsing Health Science Research Advance Educational Institute & Research Centre, 2020 9(2021), 4 (DE-627)1737949660 23103841 nnns volume:9 year:2021 number:4 https://doaj.org/article/6b05c8984fda4e7f9150d4883f46bc3e kostenfrei http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/587 kostenfrei https://doaj.org/toc/2307-3748 Journal toc kostenfrei https://doaj.org/toc/2310-3841 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4 |
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(DE-627)DOAJ018336124 (DE-599)DOAJ6b05c8984fda4e7f9150d4883f46bc3e DE-627 ger DE-627 rakwb eng Shajeela Iram verfasserin aut Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. Medicine R Tabinda Khawaja verfasserin aut Mehmood ul Hassan verfasserin aut Muhammad Jawad Khan verfasserin aut Tanveer Abbas verfasserin aut In International Journal of Endorsing Health Science Research Advance Educational Institute & Research Centre, 2020 9(2021), 4 (DE-627)1737949660 23103841 nnns volume:9 year:2021 number:4 https://doaj.org/article/6b05c8984fda4e7f9150d4883f46bc3e kostenfrei http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/587 kostenfrei https://doaj.org/toc/2307-3748 Journal toc kostenfrei https://doaj.org/toc/2310-3841 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4 |
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(DE-627)DOAJ018336124 (DE-599)DOAJ6b05c8984fda4e7f9150d4883f46bc3e DE-627 ger DE-627 rakwb eng Shajeela Iram verfasserin aut Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation. Medicine R Tabinda Khawaja verfasserin aut Mehmood ul Hassan verfasserin aut Muhammad Jawad Khan verfasserin aut Tanveer Abbas verfasserin aut In International Journal of Endorsing Health Science Research Advance Educational Institute & Research Centre, 2020 9(2021), 4 (DE-627)1737949660 23103841 nnns volume:9 year:2021 number:4 https://doaj.org/article/6b05c8984fda4e7f9150d4883f46bc3e kostenfrei http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/587 kostenfrei https://doaj.org/toc/2307-3748 Journal toc kostenfrei https://doaj.org/toc/2310-3841 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4 |
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Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan Food-Borne Illness, Bacterial Contamination, Total Viable Count, Seasonal Variation |
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bacterial load determination of poultry feed with seasonality effect in karachi, pakistan |
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Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. |
abstract |
Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. |
abstractGer |
Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. |
abstract_unstemmed |
Background: The escalating rate of food-borne diseases via poultry feeds necessitates the characterization of the pathogens to reduce the health risk factor for humans and animals. The poultry feed microbial analysis help to control food-borne illness. In addition, the knowledge about seasonal effects on microbial growth helps the manufacturers to take precautionary measures in alarming months to maintain the standard quality of poultry feed. Methodology: This study was designed to identify and enumerate bacteria and their seasonal variation. Total feed samples (n=204) were evaluated through traditional culture techniques, microscopic inspection, and biochemical properties. However, bacterial load was determined by using the total viable count. Results: As a result, five genera, including Salmonella enterica (39.05%), Escherichia coli (22.48%), Bacillus subtilis (18.34%), Staphylococcus aureus (11.24%), and Streptococcus sp (8.87%), were isolated. The proportion of occurrence of the bacterial load was lowest in December-February (64.4%) and highest in June-August (96.2%). Whereas in September-November was (87.6%) and March-May (77.5%). On the whole, the total percentage of positive samples was 82.8%. Statistical analysis revealed that (9.9×10-8 cfu/g) was the highest viable bacterial count recorded from June to August. The presence of food-borne pathogens, especially S.enterica and E.coli, is bothersome. Moreover, June to August is considered the most troubling month due to the elevated level of contamination. Conclusion: To evade microbial contamination, the microbiological security rules must be followed throughout the process of formulation and storage period, especially in sensitive hot and humid months of June to August. Also, standard inspection should be taken to control the dissemination of food-borne illness. |
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Bacterial load determination of poultry feed with seasonality effect in Karachi, Pakistan. |
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