Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also...
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Autor*in:	Kubicz Ewelina [verfasserIn] Jasińska Bożena [verfasserIn] Zgardzińska Bożena [verfasserIn] Bednarski Tomasz [verfasserIn] Białas Piotr [verfasserIn] Czerwiński Eryk [verfasserIn] Gajos Aleksander [verfasserIn] Gorgol Marek [verfasserIn] Kamińska Daria [verfasserIn] Kapłon Łukasz [verfasserIn] Kochanowski Andrzej [verfasserIn] Korcyl Grzegorz [verfasserIn] Kowalski Paweł [verfasserIn] Kozik Tomasz [verfasserIn] Krzemień Wojciech [verfasserIn] Niedźwiecki Szymon [verfasserIn] Pałka Marek [verfasserIn] Raczyński Lech [verfasserIn] Rajfur Zenon [verfasserIn] Rudy Zbigniew [verfasserIn] Rundel Oleksandr [verfasserIn] Sharma Neha Gupta [verfasserIn] Silarski Michał [verfasserIn] Słomski Artur [verfasserIn] Strzelecki Adam [verfasserIn] Wieczorek Anna [verfasserIn] Wiślicki Wojciech [verfasserIn] Zieliński Marcin [verfasserIn] Moskal Paweł [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts

Übergeordnetes Werk:	In: Nukleonika - Sciendo, 2015, 60(2015), 4, Seite 749-753
Übergeordnetes Werk:	volume:60 ; year:2015 ; number:4 ; pages:749-753

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1515/nuka-2015-0135

Katalog-ID:	DOAJ018633277

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520			\|a Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
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allfields	10.1515/nuka-2015-0135 doi (DE-627)DOAJ018633277 (DE-599)DOAJ631b261945604d4d93fa1d87f667afd8 DE-627 ger DE-627 rakwb eng Kubicz Ewelina verfasserin aut Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q Jasińska Bożena verfasserin aut Zgardzińska Bożena verfasserin aut Bednarski Tomasz verfasserin aut Białas Piotr verfasserin aut Czerwiński Eryk verfasserin aut Gajos Aleksander verfasserin aut Gorgol Marek verfasserin aut Kamińska Daria verfasserin aut Kapłon Łukasz verfasserin aut Kochanowski Andrzej verfasserin aut Korcyl Grzegorz verfasserin aut Kowalski Paweł verfasserin aut Kozik Tomasz verfasserin aut Krzemień Wojciech verfasserin aut Niedźwiecki Szymon verfasserin aut Pałka Marek verfasserin aut Raczyński Lech verfasserin aut Rajfur Zenon verfasserin aut Rudy Zbigniew verfasserin aut Rundel Oleksandr verfasserin aut Sharma Neha Gupta verfasserin aut Silarski Michał verfasserin aut Słomski Artur verfasserin aut Strzelecki Adam verfasserin aut Wieczorek Anna verfasserin aut Wiślicki Wojciech verfasserin aut Zieliński Marcin verfasserin aut Moskal Paweł verfasserin aut In Nukleonika Sciendo, 2015 60(2015), 4, Seite 749-753 (DE-627)518634310 (DE-600)2253628-0 15085791 nnns volume:60 year:2015 number:4 pages:749-753 https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 kostenfrei https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/toc/0029-5922 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 60 2015 4 749-753
spelling	10.1515/nuka-2015-0135 doi (DE-627)DOAJ018633277 (DE-599)DOAJ631b261945604d4d93fa1d87f667afd8 DE-627 ger DE-627 rakwb eng Kubicz Ewelina verfasserin aut Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q Jasińska Bożena verfasserin aut Zgardzińska Bożena verfasserin aut Bednarski Tomasz verfasserin aut Białas Piotr verfasserin aut Czerwiński Eryk verfasserin aut Gajos Aleksander verfasserin aut Gorgol Marek verfasserin aut Kamińska Daria verfasserin aut Kapłon Łukasz verfasserin aut Kochanowski Andrzej verfasserin aut Korcyl Grzegorz verfasserin aut Kowalski Paweł verfasserin aut Kozik Tomasz verfasserin aut Krzemień Wojciech verfasserin aut Niedźwiecki Szymon verfasserin aut Pałka Marek verfasserin aut Raczyński Lech verfasserin aut Rajfur Zenon verfasserin aut Rudy Zbigniew verfasserin aut Rundel Oleksandr verfasserin aut Sharma Neha Gupta verfasserin aut Silarski Michał verfasserin aut Słomski Artur verfasserin aut Strzelecki Adam verfasserin aut Wieczorek Anna verfasserin aut Wiślicki Wojciech verfasserin aut Zieliński Marcin verfasserin aut Moskal Paweł verfasserin aut In Nukleonika Sciendo, 2015 60(2015), 4, Seite 749-753 (DE-627)518634310 (DE-600)2253628-0 15085791 nnns volume:60 year:2015 number:4 pages:749-753 https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 kostenfrei https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/toc/0029-5922 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 60 2015 4 749-753
allfields_unstemmed	10.1515/nuka-2015-0135 doi (DE-627)DOAJ018633277 (DE-599)DOAJ631b261945604d4d93fa1d87f667afd8 DE-627 ger DE-627 rakwb eng Kubicz Ewelina verfasserin aut Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q Jasińska Bożena verfasserin aut Zgardzińska Bożena verfasserin aut Bednarski Tomasz verfasserin aut Białas Piotr verfasserin aut Czerwiński Eryk verfasserin aut Gajos Aleksander verfasserin aut Gorgol Marek verfasserin aut Kamińska Daria verfasserin aut Kapłon Łukasz verfasserin aut Kochanowski Andrzej verfasserin aut Korcyl Grzegorz verfasserin aut Kowalski Paweł verfasserin aut Kozik Tomasz verfasserin aut Krzemień Wojciech verfasserin aut Niedźwiecki Szymon verfasserin aut Pałka Marek verfasserin aut Raczyński Lech verfasserin aut Rajfur Zenon verfasserin aut Rudy Zbigniew verfasserin aut Rundel Oleksandr verfasserin aut Sharma Neha Gupta verfasserin aut Silarski Michał verfasserin aut Słomski Artur verfasserin aut Strzelecki Adam verfasserin aut Wieczorek Anna verfasserin aut Wiślicki Wojciech verfasserin aut Zieliński Marcin verfasserin aut Moskal Paweł verfasserin aut In Nukleonika Sciendo, 2015 60(2015), 4, Seite 749-753 (DE-627)518634310 (DE-600)2253628-0 15085791 nnns volume:60 year:2015 number:4 pages:749-753 https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 kostenfrei https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/toc/0029-5922 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 60 2015 4 749-753
allfieldsGer	10.1515/nuka-2015-0135 doi (DE-627)DOAJ018633277 (DE-599)DOAJ631b261945604d4d93fa1d87f667afd8 DE-627 ger DE-627 rakwb eng Kubicz Ewelina verfasserin aut Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q Jasińska Bożena verfasserin aut Zgardzińska Bożena verfasserin aut Bednarski Tomasz verfasserin aut Białas Piotr verfasserin aut Czerwiński Eryk verfasserin aut Gajos Aleksander verfasserin aut Gorgol Marek verfasserin aut Kamińska Daria verfasserin aut Kapłon Łukasz verfasserin aut Kochanowski Andrzej verfasserin aut Korcyl Grzegorz verfasserin aut Kowalski Paweł verfasserin aut Kozik Tomasz verfasserin aut Krzemień Wojciech verfasserin aut Niedźwiecki Szymon verfasserin aut Pałka Marek verfasserin aut Raczyński Lech verfasserin aut Rajfur Zenon verfasserin aut Rudy Zbigniew verfasserin aut Rundel Oleksandr verfasserin aut Sharma Neha Gupta verfasserin aut Silarski Michał verfasserin aut Słomski Artur verfasserin aut Strzelecki Adam verfasserin aut Wieczorek Anna verfasserin aut Wiślicki Wojciech verfasserin aut Zieliński Marcin verfasserin aut Moskal Paweł verfasserin aut In Nukleonika Sciendo, 2015 60(2015), 4, Seite 749-753 (DE-627)518634310 (DE-600)2253628-0 15085791 nnns volume:60 year:2015 number:4 pages:749-753 https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 kostenfrei https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/toc/0029-5922 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 60 2015 4 749-753
allfieldsSound	10.1515/nuka-2015-0135 doi (DE-627)DOAJ018633277 (DE-599)DOAJ631b261945604d4d93fa1d87f667afd8 DE-627 ger DE-627 rakwb eng Kubicz Ewelina verfasserin aut Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images. environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q Jasińska Bożena verfasserin aut Zgardzińska Bożena verfasserin aut Bednarski Tomasz verfasserin aut Białas Piotr verfasserin aut Czerwiński Eryk verfasserin aut Gajos Aleksander verfasserin aut Gorgol Marek verfasserin aut Kamińska Daria verfasserin aut Kapłon Łukasz verfasserin aut Kochanowski Andrzej verfasserin aut Korcyl Grzegorz verfasserin aut Kowalski Paweł verfasserin aut Kozik Tomasz verfasserin aut Krzemień Wojciech verfasserin aut Niedźwiecki Szymon verfasserin aut Pałka Marek verfasserin aut Raczyński Lech verfasserin aut Rajfur Zenon verfasserin aut Rudy Zbigniew verfasserin aut Rundel Oleksandr verfasserin aut Sharma Neha Gupta verfasserin aut Silarski Michał verfasserin aut Słomski Artur verfasserin aut Strzelecki Adam verfasserin aut Wieczorek Anna verfasserin aut Wiślicki Wojciech verfasserin aut Zieliński Marcin verfasserin aut Moskal Paweł verfasserin aut In Nukleonika Sciendo, 2015 60(2015), 4, Seite 749-753 (DE-627)518634310 (DE-600)2253628-0 15085791 nnns volume:60 year:2015 number:4 pages:749-753 https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 kostenfrei https://doi.org/10.1515/nuka-2015-0135 kostenfrei https://doaj.org/toc/0029-5922 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 60 2015 4 749-753
language	English
source	In Nukleonika 60(2015), 4, Seite 749-753 volume:60 year:2015 number:4 pages:749-753
sourceStr	In Nukleonika 60(2015), 4, Seite 749-753 volume:60 year:2015 number:4 pages:749-753
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	environmental scanning electron microscopy (esem) free volume inverted microscopy positron annihilation positronium yeasts Science Q
isfreeaccess_bool	true
container_title	Nukleonika
authorswithroles_txt_mv	Kubicz Ewelina @@aut@@ Jasińska Bożena @@aut@@ Zgardzińska Bożena @@aut@@ Bednarski Tomasz @@aut@@ Białas Piotr @@aut@@ Czerwiński Eryk @@aut@@ Gajos Aleksander @@aut@@ Gorgol Marek @@aut@@ Kamińska Daria @@aut@@ Kapłon Łukasz @@aut@@ Kochanowski Andrzej @@aut@@ Korcyl Grzegorz @@aut@@ Kowalski Paweł @@aut@@ Kozik Tomasz @@aut@@ Krzemień Wojciech @@aut@@ Niedźwiecki Szymon @@aut@@ Pałka Marek @@aut@@ Raczyński Lech @@aut@@ Rajfur Zenon @@aut@@ Rudy Zbigniew @@aut@@ Rundel Oleksandr @@aut@@ Sharma Neha Gupta @@aut@@ Silarski Michał @@aut@@ Słomski Artur @@aut@@ Strzelecki Adam @@aut@@ Wieczorek Anna @@aut@@ Wiślicki Wojciech @@aut@@ Zieliński Marcin @@aut@@ Moskal Paweł @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	518634310
id	DOAJ018633277
language_de	englisch
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author	Kubicz Ewelina
spellingShingle	Kubicz Ewelina misc environmental scanning electron microscopy (esem) misc free volume misc inverted microscopy misc positron annihilation misc positronium misc yeasts misc Science misc Q Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
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title	Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
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title_full	Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
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title_sort	studies of unicellular microorganisms saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
title_auth	Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
abstract	Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
abstractGer	Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
abstract_unstemmed	Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
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title_short	Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
url	https://doi.org/10.1515/nuka-2015-0135 https://doaj.org/article/631b261945604d4d93fa1d87f667afd8 https://doaj.org/toc/0029-5922
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Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

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