Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133

Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some anti...
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Autor*in:	Josephine S. Gnanandarajah [verfasserIn] Peter A. Gillis [verfasserIn] Nelmary Hernandez-Alvarado [verfasserIn] LeeAnn Higgins [verfasserIn] Todd W. Markowski [verfasserIn] Heungsup Sung [verfasserIn] Sheila Lumley [verfasserIn] Mark R. Schleiss [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 6(2014), 2, Seite 727-751
Übergeordnetes Werk:	volume:6 ; year:2014 ; number:2 ; pages:727-751

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v6020727

Katalog-ID:	DOAJ018738435

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allfields	10.3390/v6020727 doi (DE-627)DOAJ018738435 (DE-599)DOAJ136a9d8ed58a433dbd36574c62a94a9e DE-627 ger DE-627 rakwb eng QR1-502 Josephine S. Gnanandarajah verfasserin aut Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model. guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus Microbiology Peter A. Gillis verfasserin aut Nelmary Hernandez-Alvarado verfasserin aut LeeAnn Higgins verfasserin aut Todd W. Markowski verfasserin aut Heungsup Sung verfasserin aut Sheila Lumley verfasserin aut Mark R. Schleiss verfasserin aut In Viruses MDPI AG, 2009 6(2014), 2, Seite 727-751 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:6 year:2014 number:2 pages:727-751 https://doi.org/10.3390/v6020727 kostenfrei https://doaj.org/article/136a9d8ed58a433dbd36574c62a94a9e kostenfrei http://www.mdpi.com/1999-4915/6/2/727 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2014 2 727-751
spelling	10.3390/v6020727 doi (DE-627)DOAJ018738435 (DE-599)DOAJ136a9d8ed58a433dbd36574c62a94a9e DE-627 ger DE-627 rakwb eng QR1-502 Josephine S. Gnanandarajah verfasserin aut Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model. guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus Microbiology Peter A. Gillis verfasserin aut Nelmary Hernandez-Alvarado verfasserin aut LeeAnn Higgins verfasserin aut Todd W. Markowski verfasserin aut Heungsup Sung verfasserin aut Sheila Lumley verfasserin aut Mark R. Schleiss verfasserin aut In Viruses MDPI AG, 2009 6(2014), 2, Seite 727-751 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:6 year:2014 number:2 pages:727-751 https://doi.org/10.3390/v6020727 kostenfrei https://doaj.org/article/136a9d8ed58a433dbd36574c62a94a9e kostenfrei http://www.mdpi.com/1999-4915/6/2/727 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2014 2 727-751
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allfieldsGer	10.3390/v6020727 doi (DE-627)DOAJ018738435 (DE-599)DOAJ136a9d8ed58a433dbd36574c62a94a9e DE-627 ger DE-627 rakwb eng QR1-502 Josephine S. Gnanandarajah verfasserin aut Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model. guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus Microbiology Peter A. Gillis verfasserin aut Nelmary Hernandez-Alvarado verfasserin aut LeeAnn Higgins verfasserin aut Todd W. Markowski verfasserin aut Heungsup Sung verfasserin aut Sheila Lumley verfasserin aut Mark R. Schleiss verfasserin aut In Viruses MDPI AG, 2009 6(2014), 2, Seite 727-751 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:6 year:2014 number:2 pages:727-751 https://doi.org/10.3390/v6020727 kostenfrei https://doaj.org/article/136a9d8ed58a433dbd36574c62a94a9e kostenfrei http://www.mdpi.com/1999-4915/6/2/727 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2014 2 727-751
allfieldsSound	10.3390/v6020727 doi (DE-627)DOAJ018738435 (DE-599)DOAJ136a9d8ed58a433dbd36574c62a94a9e DE-627 ger DE-627 rakwb eng QR1-502 Josephine S. Gnanandarajah verfasserin aut Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model. guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus Microbiology Peter A. Gillis verfasserin aut Nelmary Hernandez-Alvarado verfasserin aut LeeAnn Higgins verfasserin aut Todd W. Markowski verfasserin aut Heungsup Sung verfasserin aut Sheila Lumley verfasserin aut Mark R. Schleiss verfasserin aut In Viruses MDPI AG, 2009 6(2014), 2, Seite 727-751 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:6 year:2014 number:2 pages:727-751 https://doi.org/10.3390/v6020727 kostenfrei https://doaj.org/article/136a9d8ed58a433dbd36574c62a94a9e kostenfrei http://www.mdpi.com/1999-4915/6/2/727 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2014 2 727-751
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source	In Viruses 6(2014), 2, Seite 727-751 volume:6 year:2014 number:2 pages:727-751
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authorswithroles_txt_mv	Josephine S. Gnanandarajah @@aut@@ Peter A. Gillis @@aut@@ Nelmary Hernandez-Alvarado @@aut@@ LeeAnn Higgins @@aut@@ Todd W. Markowski @@aut@@ Heungsup Sung @@aut@@ Sheila Lumley @@aut@@ Mark R. Schleiss @@aut@@
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callnumber-first	Q - Science
author	Josephine S. Gnanandarajah
spellingShingle	Josephine S. Gnanandarajah misc QR1-502 misc guinea pig cytomegalovirus misc cytomegalovirus vaccine misc pentameric complex misc congenital cytomegalovirus infection misc baculovirus misc Microbiology Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133
authorStr	Josephine S. Gnanandarajah
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topic_title	QR1-502 Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133 guinea pig cytomegalovirus cytomegalovirus vaccine pentameric complex congenital cytomegalovirus infection baculovirus
topic	misc QR1-502 misc guinea pig cytomegalovirus misc cytomegalovirus vaccine misc pentameric complex misc congenital cytomegalovirus infection misc baculovirus misc Microbiology
topic_unstemmed	misc QR1-502 misc guinea pig cytomegalovirus misc cytomegalovirus vaccine misc pentameric complex misc congenital cytomegalovirus infection misc baculovirus misc Microbiology
topic_browse	misc QR1-502 misc guinea pig cytomegalovirus misc cytomegalovirus vaccine misc pentameric complex misc congenital cytomegalovirus infection misc baculovirus misc Microbiology
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title	Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133
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title_full	Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133
author_sort	Josephine S. Gnanandarajah
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author_browse	Josephine S. Gnanandarajah Peter A. Gillis Nelmary Hernandez-Alvarado LeeAnn Higgins Todd W. Markowski Heungsup Sung Sheila Lumley Mark R. Schleiss
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author-letter	Josephine S. Gnanandarajah
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title_sort	identification by mass spectrometry and immune response analysis of guinea pig cytomegalovirus (gpcmv) pentameric complex proteins gp129, 131 and 133
callnumber	QR1-502
title_auth	Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133
abstract	Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model.
abstractGer	Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model.
abstract_unstemmed	Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model.
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title_short	Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133
url	https://doi.org/10.3390/v6020727 https://doaj.org/article/136a9d8ed58a433dbd36574c62a94a9e http://www.mdpi.com/1999-4915/6/2/727 https://doaj.org/toc/1999-4915
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author2	Peter A. Gillis Nelmary Hernandez-Alvarado LeeAnn Higgins Todd W. Markowski Heungsup Sung Sheila Lumley Mark R. Schleiss
author2Str	Peter A. Gillis Nelmary Hernandez-Alvarado LeeAnn Higgins Todd W. Markowski Heungsup Sung Sheila Lumley Mark R. Schleiss
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up_date	2024-07-03T19:39:30.995Z
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Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133

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