Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation

OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	André Luiz de Souza Grava [verfasserIn] Luiz Fernando Ferrari [verfasserIn] Carlos Amílcar Parada [verfasserIn] Helton L.A. Defino [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2008

Schlagwörter:	Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement

Übergeordnetes Werk:	In: Revista Brasileira de Ortopedia - Thieme Revinter Publicações Ltda., 2007, 43(2008), 4, Seite 116-125
Übergeordnetes Werk:	volume:43 ; year:2008 ; number:4 ; pages:116-125

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1590/S0102-36162008000300003

Katalog-ID:	DOAJ01925007X

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ01925007X
003	DE-627
005	20240413224902.0
007	cr uuu---uuuuu
008	230226s2008 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1590/S0102-36162008000300003 \|2 doi
035			\|a (DE-627)DOAJ01925007X
035			\|a (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng \|a por
050		0	\|a RD701-811
100	0		\|a André Luiz de Souza Grava \|e verfasserin \|4 aut
245	1	0	\|a Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
264		1	\|c 2008
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.
650		4	\|a Coluna vertebral
650		4	\|a Disco intervertebral
650		4	\|a Dor lombar
650		4	\|a Hiperalgesia
650		4	\|a Deslocamento do disco intervertebral
650		4	\|a Ratos Wistar
650		4	\|a Spine
650		4	\|a Intervertebral disk
650		4	\|a Low back pain
650		4	\|a Hyperalgesia
650		4	\|a Intervertebral disk displacement
653		0	\|a Medicine
653		0	\|a R
653		0	\|a Orthopedic surgery
700	0		\|a Luiz Fernando Ferrari \|e verfasserin \|4 aut
700	0		\|a Carlos Amílcar Parada \|e verfasserin \|4 aut
700	0		\|a Helton L.A. Defino \|e verfasserin \|4 aut
773	0	8	\|i In \|t Revista Brasileira de Ortopedia \|d Thieme Revinter Publicações Ltda., 2007 \|g 43(2008), 4, Seite 116-125 \|w (DE-627)559077734 \|w (DE-600)2411301-3 \|x 19824378 \|7 nnns
773	1	8	\|g volume:43 \|g year:2008 \|g number:4 \|g pages:116-125
856	4	0	\|u https://doi.org/10.1590/S0102-36162008000300003 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a \|z kostenfrei
856	4	0	\|u http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/0102-3616 \|y Journal toc \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1982-4378 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 43 \|j 2008 \|e 4 \|h 116-125

Indexfelder

author_variant	a l d s g aldsg l f f lff c a p cap h l d hld
matchkey_str	article:19824378:2008----::oeoxeietlaaetddhnaoicitretbaeprmnamdlotd
hierarchy_sort_str	2008
callnumber-subject-code	RD
publishDate	2008
allfields	10.1590/S0102-36162008000300003 doi (DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a DE-627 ger DE-627 rakwb eng por RD701-811 André Luiz de Souza Grava verfasserin aut Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery Luiz Fernando Ferrari verfasserin aut Carlos Amílcar Parada verfasserin aut Helton L.A. Defino verfasserin aut In Revista Brasileira de Ortopedia Thieme Revinter Publicações Ltda., 2007 43(2008), 4, Seite 116-125 (DE-627)559077734 (DE-600)2411301-3 19824378 nnns volume:43 year:2008 number:4 pages:116-125 https://doi.org/10.1590/S0102-36162008000300003 kostenfrei https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 kostenfrei https://doaj.org/toc/0102-3616 Journal toc kostenfrei https://doaj.org/toc/1982-4378 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2008 4 116-125
spelling	10.1590/S0102-36162008000300003 doi (DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a DE-627 ger DE-627 rakwb eng por RD701-811 André Luiz de Souza Grava verfasserin aut Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery Luiz Fernando Ferrari verfasserin aut Carlos Amílcar Parada verfasserin aut Helton L.A. Defino verfasserin aut In Revista Brasileira de Ortopedia Thieme Revinter Publicações Ltda., 2007 43(2008), 4, Seite 116-125 (DE-627)559077734 (DE-600)2411301-3 19824378 nnns volume:43 year:2008 number:4 pages:116-125 https://doi.org/10.1590/S0102-36162008000300003 kostenfrei https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 kostenfrei https://doaj.org/toc/0102-3616 Journal toc kostenfrei https://doaj.org/toc/1982-4378 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2008 4 116-125
allfields_unstemmed	10.1590/S0102-36162008000300003 doi (DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a DE-627 ger DE-627 rakwb eng por RD701-811 André Luiz de Souza Grava verfasserin aut Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery Luiz Fernando Ferrari verfasserin aut Carlos Amílcar Parada verfasserin aut Helton L.A. Defino verfasserin aut In Revista Brasileira de Ortopedia Thieme Revinter Publicações Ltda., 2007 43(2008), 4, Seite 116-125 (DE-627)559077734 (DE-600)2411301-3 19824378 nnns volume:43 year:2008 number:4 pages:116-125 https://doi.org/10.1590/S0102-36162008000300003 kostenfrei https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 kostenfrei https://doaj.org/toc/0102-3616 Journal toc kostenfrei https://doaj.org/toc/1982-4378 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2008 4 116-125
allfieldsGer	10.1590/S0102-36162008000300003 doi (DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a DE-627 ger DE-627 rakwb eng por RD701-811 André Luiz de Souza Grava verfasserin aut Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery Luiz Fernando Ferrari verfasserin aut Carlos Amílcar Parada verfasserin aut Helton L.A. Defino verfasserin aut In Revista Brasileira de Ortopedia Thieme Revinter Publicações Ltda., 2007 43(2008), 4, Seite 116-125 (DE-627)559077734 (DE-600)2411301-3 19824378 nnns volume:43 year:2008 number:4 pages:116-125 https://doi.org/10.1590/S0102-36162008000300003 kostenfrei https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 kostenfrei https://doaj.org/toc/0102-3616 Journal toc kostenfrei https://doaj.org/toc/1982-4378 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2008 4 116-125
allfieldsSound	10.1590/S0102-36162008000300003 doi (DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a DE-627 ger DE-627 rakwb eng por RD701-811 André Luiz de Souza Grava verfasserin aut Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used. Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery Luiz Fernando Ferrari verfasserin aut Carlos Amílcar Parada verfasserin aut Helton L.A. Defino verfasserin aut In Revista Brasileira de Ortopedia Thieme Revinter Publicações Ltda., 2007 43(2008), 4, Seite 116-125 (DE-627)559077734 (DE-600)2411301-3 19824378 nnns volume:43 year:2008 number:4 pages:116-125 https://doi.org/10.1590/S0102-36162008000300003 kostenfrei https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 kostenfrei https://doaj.org/toc/0102-3616 Journal toc kostenfrei https://doaj.org/toc/1982-4378 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 43 2008 4 116-125
language	English Portuguese
source	In Revista Brasileira de Ortopedia 43(2008), 4, Seite 116-125 volume:43 year:2008 number:4 pages:116-125
sourceStr	In Revista Brasileira de Ortopedia 43(2008), 4, Seite 116-125 volume:43 year:2008 number:4 pages:116-125
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement Medicine R Orthopedic surgery
isfreeaccess_bool	true
container_title	Revista Brasileira de Ortopedia
authorswithroles_txt_mv	André Luiz de Souza Grava @@aut@@ Luiz Fernando Ferrari @@aut@@ Carlos Amílcar Parada @@aut@@ Helton L.A. Defino @@aut@@
publishDateDaySort_date	2008-01-01T00:00:00Z
hierarchy_top_id	559077734
id	DOAJ01925007X
language_de	englisch portugiesisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ01925007X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240413224902.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2008 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1590/S0102-36162008000300003</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ01925007X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield><subfield code="a">por</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RD701-811</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">André Luiz de Souza Grava</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Coluna vertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Disco intervertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dor lombar</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hiperalgesia</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Deslocamento do disco intervertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ratos Wistar</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intervertebral disk</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Low back pain</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hyperalgesia</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intervertebral disk displacement</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Orthopedic surgery</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Luiz Fernando Ferrari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carlos Amílcar Parada</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Helton L.A. Defino</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Revista Brasileira de Ortopedia</subfield><subfield code="d">Thieme Revinter Publicações Ltda., 2007</subfield><subfield code="g">43(2008), 4, Seite 116-125</subfield><subfield code="w">(DE-627)559077734</subfield><subfield code="w">(DE-600)2411301-3</subfield><subfield code="x">19824378</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:43</subfield><subfield code="g">year:2008</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:116-125</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1590/S0102-36162008000300003</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0102-3616</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1982-4378</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">43</subfield><subfield code="j">2008</subfield><subfield code="e">4</subfield><subfield code="h">116-125</subfield></datafield></record></collection>
callnumber-first	R - Medicine
author	André Luiz de Souza Grava
spellingShingle	André Luiz de Souza Grava misc RD701-811 misc Coluna vertebral misc Disco intervertebral misc Dor lombar misc Hiperalgesia misc Deslocamento do disco intervertebral misc Ratos Wistar misc Spine misc Intervertebral disk misc Low back pain misc Hyperalgesia misc Intervertebral disk displacement misc Medicine misc R misc Orthopedic surgery Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
authorStr	André Luiz de Souza Grava
ppnlink_with_tag_str_mv	@@773@@(DE-627)559077734
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	RD701-811
illustrated	Not Illustrated
issn	19824378
topic_title	RD701-811 Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation Coluna vertebral Disco intervertebral Dor lombar Hiperalgesia Deslocamento do disco intervertebral Ratos Wistar Spine Intervertebral disk Low back pain Hyperalgesia Intervertebral disk displacement
topic	misc RD701-811 misc Coluna vertebral misc Disco intervertebral misc Dor lombar misc Hiperalgesia misc Deslocamento do disco intervertebral misc Ratos Wistar misc Spine misc Intervertebral disk misc Low back pain misc Hyperalgesia misc Intervertebral disk displacement misc Medicine misc R misc Orthopedic surgery
topic_unstemmed	misc RD701-811 misc Coluna vertebral misc Disco intervertebral misc Dor lombar misc Hiperalgesia misc Deslocamento do disco intervertebral misc Ratos Wistar misc Spine misc Intervertebral disk misc Low back pain misc Hyperalgesia misc Intervertebral disk displacement misc Medicine misc R misc Orthopedic surgery
topic_browse	misc RD701-811 misc Coluna vertebral misc Disco intervertebral misc Dor lombar misc Hiperalgesia misc Deslocamento do disco intervertebral misc Ratos Wistar misc Spine misc Intervertebral disk misc Low back pain misc Hyperalgesia misc Intervertebral disk displacement misc Medicine misc R misc Orthopedic surgery
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Revista Brasileira de Ortopedia
hierarchy_parent_id	559077734
hierarchy_top_title	Revista Brasileira de Ortopedia
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)559077734 (DE-600)2411301-3
title	Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
ctrlnum	(DE-627)DOAJ01925007X (DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a
title_full	Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
author_sort	André Luiz de Souza Grava
journal	Revista Brasileira de Ortopedia
journalStr	Revista Brasileira de Ortopedia
callnumber-first-code	R
lang_code	eng por
isOA_bool	true
recordtype	marc
publishDateSort	2008
contenttype_str_mv	txt
container_start_page	116
author_browse	André Luiz de Souza Grava Luiz Fernando Ferrari Carlos Amílcar Parada Helton L.A. Defino
container_volume	43
class	RD701-811
format_se	Elektronische Aufsätze
author-letter	André Luiz de Souza Grava
doi_str_mv	10.1590/S0102-36162008000300003
author2-role	verfasserin
title_sort	modelo experimental para o estudo da hérnia do disco intervertebral experimental model to study intervertebral disc herniation
callnumber	RD701-811
title_auth	Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
abstract	OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.
abstractGer	OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.
abstract_unstemmed	OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4
title_short	Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation
url	https://doi.org/10.1590/S0102-36162008000300003 https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003 https://doaj.org/toc/0102-3616 https://doaj.org/toc/1982-4378
remote_bool	true
author2	Luiz Fernando Ferrari Carlos Amílcar Parada Helton L.A. Defino
author2Str	Luiz Fernando Ferrari Carlos Amílcar Parada Helton L.A. Defino
ppnlink	559077734
callnumber-subject	RD - Surgery
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1590/S0102-36162008000300003
callnumber-a	RD701-811
up_date	2024-07-03T22:36:20.954Z
_version_	1803599144242118656
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ01925007X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240413224902.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2008 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1590/S0102-36162008000300003</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ01925007X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ624c36de4355472e9feb15e6ebd9032a</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield><subfield code="a">por</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RD701-811</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">André Luiz de Souza Grava</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">OBJETIVO: Apresentar um modelo experimental de hérnia de disco e sua validação para estudo da hiperalgesia mecânica e térmica produzidas pelo contato do núcleo pulposo (NP) com as estruturas nervosas envolvidas nessa afecção. MÉTODOS: Foram utilizados ratos Wistar, sendo o NP autólogo retirado da região sacrococcígea e depositado sobre a dura-máter, raiz nervosa ou gânglios das raízes dorsais L4, L5 ou L6. Os experimentos foram divididos em quatro etapas: 1ª) determinação da estrutura nervosa mais sensível ao contato com o NP; 2ª) identificação do melhor nível lombar para a indução da hiperalgesia; 3ª) determinação da ausência de lesão motora; e 4ª) determinação da influência do procedimento cirúrgico no desenvolvimento do processo inflamatório. A hiperalgesia foi avaliada nos testes de von Frey eletrônico e de Hargreaves e a função motora, pelo teste de rota-rod. RESULTADOS: O NP induziu hiperalgesia de maior intensidade na pata quando em contato com o gânglio da raiz dorsal (GRD) do que em contato com a dura-máter ou a raiz nervosa. Quando em contato com o GRD-L5, o NP induziu hiperalgesia ainda maior que a induzida pelo contato com os GRDs L4 e L6. Não foram observadas lesão motora e influência do processo inflamatório cirúrgico sobre a hiperalgesia. CONCLUSÃO: O GRD é a estrutura mais sensível aos componentes do NP para a produção da hiperalgesia, sendo o quinto nível lombar o que apresentou maior alteração nas sensibilidades mecânica e térmica avaliadas na pata dos animais, de acordo com os métodos utilizados.<br<OBJECTIVE:The purpose of this study is to present an experimental model of disc herniation and to validate such model to study mechanic and thermal hyperalgesia produced by the contact of the nucleus pulposus (NP) with nerve structures involved in this condition. METHODS: The authors used Wistar rats, the autologous NP being removed from the sacrococcygeal region and deposited on the dura mater, nerve root, or L4, L5, or L6 dorsal root ganglia. The experiments were divided into four steps: 1) determining the nerve structure that is the most sensitive to the contact with NP; 2) identifying the best lumbar level to induce hyperalgesia; 3) determining absence of a motor lesion; and 4) determining the impact of the surgical procedure upon the inflammatory process. Hyperalgesia was evaluated by the von Frey electronic test and the Hargreaves test, and the motor function was evaluated by the rota-rod test. RESULTS: NP induced higher intensity hyperalgesia in the paw when it was in contact with the dorsal root ganglion (GRD) than when it was in contact with the dura mater or the nerve root. Contact with GRD-L5, led NP to induce even higher hyperalgesia than that induced in the contact with L4 and L6 GRDs. No motor lesion and impact of the surgical inflammatory process on hyperalgesia were observed. CONCLUSION: GRD is the structure that is most sensitive to NP components to produce hyperalgesia, the fifth lumbar level being that showed the greatest change in the mechanic and thermal sensitivities evaluated in the paws of the animals, under the methods used.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Coluna vertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Disco intervertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dor lombar</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hiperalgesia</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Deslocamento do disco intervertebral</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ratos Wistar</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intervertebral disk</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Low back pain</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hyperalgesia</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intervertebral disk displacement</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Orthopedic surgery</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Luiz Fernando Ferrari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carlos Amílcar Parada</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Helton L.A. Defino</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Revista Brasileira de Ortopedia</subfield><subfield code="d">Thieme Revinter Publicações Ltda., 2007</subfield><subfield code="g">43(2008), 4, Seite 116-125</subfield><subfield code="w">(DE-627)559077734</subfield><subfield code="w">(DE-600)2411301-3</subfield><subfield code="x">19824378</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:43</subfield><subfield code="g">year:2008</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:116-125</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1590/S0102-36162008000300003</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/624c36de4355472e9feb15e6ebd9032a</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162008000300003</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0102-3616</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1982-4378</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">43</subfield><subfield code="j">2008</subfield><subfield code="e">4</subfield><subfield code="h">116-125</subfield></datafield></record></collection>
score	7.4023256

Nicht das Richtige dabei?

Schreiben Sie uns!

Modelo experimental para o estudo da hérnia do disco intervertebral Experimental model to study intervertebral disc herniation

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?