A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications

Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for se...
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Autor*in:	Huifang Liu [verfasserIn] Geun Su Noh [verfasserIn] Yange Luan [verfasserIn] Zhen Qiao [verfasserIn] Bonhan Koo [verfasserIn] Yoon Ok Jang [verfasserIn] Yong Shin [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	sample preparation nanocomposite pathogenic enrichment nucleic acid isolation

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 24(2019), 7, p 1321
Übergeordnetes Werk:	volume:24 ; year:2019 ; number:7, p 1321

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules24071321

Katalog-ID:	DOAJ019273622

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650		4	\|a sample preparation
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allfields	10.3390/molecules24071321 doi (DE-627)DOAJ019273622 (DE-599)DOAJd390b6fea86b45c4913d57a1b99af187 DE-627 ger DE-627 rakwb eng QD241-441 Huifang Liu verfasserin aut A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems. sample preparation nanocomposite pathogenic enrichment nucleic acid isolation Organic chemistry Geun Su Noh verfasserin aut Yange Luan verfasserin aut Zhen Qiao verfasserin aut Bonhan Koo verfasserin aut Yoon Ok Jang verfasserin aut Yong Shin verfasserin aut In Molecules MDPI AG, 2003 24(2019), 7, p 1321 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:24 year:2019 number:7, p 1321 https://doi.org/10.3390/molecules24071321 kostenfrei https://doaj.org/article/d390b6fea86b45c4913d57a1b99af187 kostenfrei https://www.mdpi.com/1420-3049/24/7/1321 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2019 7, p 1321
spelling	10.3390/molecules24071321 doi (DE-627)DOAJ019273622 (DE-599)DOAJd390b6fea86b45c4913d57a1b99af187 DE-627 ger DE-627 rakwb eng QD241-441 Huifang Liu verfasserin aut A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems. sample preparation nanocomposite pathogenic enrichment nucleic acid isolation Organic chemistry Geun Su Noh verfasserin aut Yange Luan verfasserin aut Zhen Qiao verfasserin aut Bonhan Koo verfasserin aut Yoon Ok Jang verfasserin aut Yong Shin verfasserin aut In Molecules MDPI AG, 2003 24(2019), 7, p 1321 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:24 year:2019 number:7, p 1321 https://doi.org/10.3390/molecules24071321 kostenfrei https://doaj.org/article/d390b6fea86b45c4913d57a1b99af187 kostenfrei https://www.mdpi.com/1420-3049/24/7/1321 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2019 7, p 1321
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allfieldsSound	10.3390/molecules24071321 doi (DE-627)DOAJ019273622 (DE-599)DOAJd390b6fea86b45c4913d57a1b99af187 DE-627 ger DE-627 rakwb eng QD241-441 Huifang Liu verfasserin aut A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems. sample preparation nanocomposite pathogenic enrichment nucleic acid isolation Organic chemistry Geun Su Noh verfasserin aut Yange Luan verfasserin aut Zhen Qiao verfasserin aut Bonhan Koo verfasserin aut Yoon Ok Jang verfasserin aut Yong Shin verfasserin aut In Molecules MDPI AG, 2003 24(2019), 7, p 1321 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:24 year:2019 number:7, p 1321 https://doi.org/10.3390/molecules24071321 kostenfrei https://doaj.org/article/d390b6fea86b45c4913d57a1b99af187 kostenfrei https://www.mdpi.com/1420-3049/24/7/1321 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2019 7, p 1321
language	English
source	In Molecules 24(2019), 7, p 1321 volume:24 year:2019 number:7, p 1321
sourceStr	In Molecules 24(2019), 7, p 1321 volume:24 year:2019 number:7, p 1321
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	sample preparation nanocomposite pathogenic enrichment nucleic acid isolation Organic chemistry
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authorswithroles_txt_mv	Huifang Liu @@aut@@ Geun Su Noh @@aut@@ Yange Luan @@aut@@ Zhen Qiao @@aut@@ Bonhan Koo @@aut@@ Yoon Ok Jang @@aut@@ Yong Shin @@aut@@
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callnumber-first	Q - Science
author	Huifang Liu
spellingShingle	Huifang Liu misc QD241-441 misc sample preparation misc nanocomposite misc pathogenic misc enrichment misc nucleic acid isolation misc Organic chemistry A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications
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topic_title	QD241-441 A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications sample preparation nanocomposite pathogenic enrichment nucleic acid isolation
topic	misc QD241-441 misc sample preparation misc nanocomposite misc pathogenic misc enrichment misc nucleic acid isolation misc Organic chemistry
topic_unstemmed	misc QD241-441 misc sample preparation misc nanocomposite misc pathogenic misc enrichment misc nucleic acid isolation misc Organic chemistry
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title	A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications
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title_sort	sample preparation technique using biocompatible composites for biomedical applications
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title_auth	A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications
abstract	Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.
abstractGer	Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.
abstract_unstemmed	Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an <i<Escherichia coli</i< concentration of 10<sup<6</sup< CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.
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title_short	A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications
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