Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)

The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< gen...
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Autor*in:	Jinqiu Li [verfasserIn] Yu Duan [verfasserIn] Zhaolan Han [verfasserIn] Xiaowen Shang [verfasserIn] Kexin Zhang [verfasserIn] Zhongwei Zou [verfasserIn] Yuanchun Ma [verfasserIn] Fang Li [verfasserIn] Wanping Fang [verfasserIn] Xujun Zhu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Pb treatment gene expression

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 10(2021), 6, p 1055
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:6, p 1055

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants10061055

Katalog-ID:	DOAJ019433727

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520			\|a The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants.
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allfields	10.3390/plants10061055 doi (DE-627)DOAJ019433727 (DE-599)DOAJ146ab61128a64314b661c34dc0a06a6b DE-627 ger DE-627 rakwb eng QK1-989 Jinqiu Li verfasserin aut Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants. <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression Botany Yu Duan verfasserin aut Zhaolan Han verfasserin aut Xiaowen Shang verfasserin aut Kexin Zhang verfasserin aut Zhongwei Zou verfasserin aut Yuanchun Ma verfasserin aut Fang Li verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Plants MDPI AG, 2013 10(2021), 6, p 1055 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:10 year:2021 number:6, p 1055 https://doi.org/10.3390/plants10061055 kostenfrei https://doaj.org/article/146ab61128a64314b661c34dc0a06a6b kostenfrei https://www.mdpi.com/2223-7747/10/6/1055 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1055
spelling	10.3390/plants10061055 doi (DE-627)DOAJ019433727 (DE-599)DOAJ146ab61128a64314b661c34dc0a06a6b DE-627 ger DE-627 rakwb eng QK1-989 Jinqiu Li verfasserin aut Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants. <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression Botany Yu Duan verfasserin aut Zhaolan Han verfasserin aut Xiaowen Shang verfasserin aut Kexin Zhang verfasserin aut Zhongwei Zou verfasserin aut Yuanchun Ma verfasserin aut Fang Li verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Plants MDPI AG, 2013 10(2021), 6, p 1055 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:10 year:2021 number:6, p 1055 https://doi.org/10.3390/plants10061055 kostenfrei https://doaj.org/article/146ab61128a64314b661c34dc0a06a6b kostenfrei https://www.mdpi.com/2223-7747/10/6/1055 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1055
allfields_unstemmed	10.3390/plants10061055 doi (DE-627)DOAJ019433727 (DE-599)DOAJ146ab61128a64314b661c34dc0a06a6b DE-627 ger DE-627 rakwb eng QK1-989 Jinqiu Li verfasserin aut Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants. <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression Botany Yu Duan verfasserin aut Zhaolan Han verfasserin aut Xiaowen Shang verfasserin aut Kexin Zhang verfasserin aut Zhongwei Zou verfasserin aut Yuanchun Ma verfasserin aut Fang Li verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Plants MDPI AG, 2013 10(2021), 6, p 1055 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:10 year:2021 number:6, p 1055 https://doi.org/10.3390/plants10061055 kostenfrei https://doaj.org/article/146ab61128a64314b661c34dc0a06a6b kostenfrei https://www.mdpi.com/2223-7747/10/6/1055 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1055
allfieldsGer	10.3390/plants10061055 doi (DE-627)DOAJ019433727 (DE-599)DOAJ146ab61128a64314b661c34dc0a06a6b DE-627 ger DE-627 rakwb eng QK1-989 Jinqiu Li verfasserin aut Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants. <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression Botany Yu Duan verfasserin aut Zhaolan Han verfasserin aut Xiaowen Shang verfasserin aut Kexin Zhang verfasserin aut Zhongwei Zou verfasserin aut Yuanchun Ma verfasserin aut Fang Li verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Plants MDPI AG, 2013 10(2021), 6, p 1055 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:10 year:2021 number:6, p 1055 https://doi.org/10.3390/plants10061055 kostenfrei https://doaj.org/article/146ab61128a64314b661c34dc0a06a6b kostenfrei https://www.mdpi.com/2223-7747/10/6/1055 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1055
allfieldsSound	10.3390/plants10061055 doi (DE-627)DOAJ019433727 (DE-599)DOAJ146ab61128a64314b661c34dc0a06a6b DE-627 ger DE-627 rakwb eng QK1-989 Jinqiu Li verfasserin aut Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants. <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression Botany Yu Duan verfasserin aut Zhaolan Han verfasserin aut Xiaowen Shang verfasserin aut Kexin Zhang verfasserin aut Zhongwei Zou verfasserin aut Yuanchun Ma verfasserin aut Fang Li verfasserin aut Wanping Fang verfasserin aut Xujun Zhu verfasserin aut In Plants MDPI AG, 2013 10(2021), 6, p 1055 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:10 year:2021 number:6, p 1055 https://doi.org/10.3390/plants10061055 kostenfrei https://doaj.org/article/146ab61128a64314b661c34dc0a06a6b kostenfrei https://www.mdpi.com/2223-7747/10/6/1055 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6, p 1055
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authorswithroles_txt_mv	Jinqiu Li @@aut@@ Yu Duan @@aut@@ Zhaolan Han @@aut@@ Xiaowen Shang @@aut@@ Kexin Zhang @@aut@@ Zhongwei Zou @@aut@@ Yuanchun Ma @@aut@@ Fang Li @@aut@@ Wanping Fang @@aut@@ Xujun Zhu @@aut@@
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callnumber-first	Q - Science
author	Jinqiu Li
spellingShingle	Jinqiu Li misc QK1-989 misc <i<Camellia sinensis</i< misc <i<NRAMP</i< misc Pb treatment misc gene expression misc Botany Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)
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topic_title	QK1-989 Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<) <i<Camellia sinensis</i< <i<NRAMP</i< Pb treatment gene expression
topic	misc QK1-989 misc <i<Camellia sinensis</i< misc <i<NRAMP</i< misc Pb treatment misc gene expression misc Botany
topic_unstemmed	misc QK1-989 misc <i<Camellia sinensis</i< misc <i<NRAMP</i< misc Pb treatment misc gene expression misc Botany
topic_browse	misc QK1-989 misc <i<Camellia sinensis</i< misc <i<NRAMP</i< misc Pb treatment misc gene expression misc Botany
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title	Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)
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title_full	Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)
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title_sort	genome-wide identification and expression analysis of the <i<nramp</i< family genes in tea plant (<i<camellia sinensis</i<)
callnumber	QK1-989
title_auth	Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)
abstract	The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants.
abstractGer	The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants.
abstract_unstemmed	The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the <i<CsNRAMP</i< family could provide primary information for further studies on the functional roles of <i<CsNRAMP</i<<i<s</i< in divalent metal transportation in tea plants.
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title_short	Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)
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Little is known about the NRAMP family in <i<Camellia sinensis</i<. In this study, 11 <i<NRAMP</i< genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 <i<CsNRAMP</i< genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, <i<CsNRAMP3</i<, <i<CsNRAMP4</i<, and <i<CsNRAMP5</i<, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of <i<CsNRAMPs</i< in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that <i<CsNRAMPs</i< were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. 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Genome-Wide Identification and Expression Analysis of the <i<NRAMP</i< Family Genes in Tea Plant (<i<Camellia sinensis</i<)

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