Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes

Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP...
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Autor*in:	Daichi Kobayashi [verfasserIn] Yuki Sugiura [verfasserIn] Eiji Umemoto [verfasserIn] Akira Takeda [verfasserIn] Hisashi Ueta [verfasserIn] Haruko Hayasaka [verfasserIn] Shinsuke Matsuzaki [verfasserIn] Tomoya Katakai [verfasserIn] Makoto Suematsu [verfasserIn] Itaru Hamachi [verfasserIn] Gennady G. Yegutkin [verfasserIn] Marko Salmi [verfasserIn] Sirpa Jalkanen [verfasserIn] Masayuki Miyasaka [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs)

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2021.786595

Katalog-ID:	DOAJ019597207

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author_variant	d k dk d k dk y s ys e u eu a t at h u hu h h hh s m sm s m sm t k tk m s ms i h ih g g y ggy m s ms m s ms s j sj m m mm m m mm m m mm
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allfields	10.3389/fimmu.2021.786595 doi (DE-627)DOAJ019597207 (DE-599)DOAJ97740f20ca8d49139c8d35c2edaa2c0f DE-627 ger DE-627 rakwb eng RC581-607 Daichi Kobayashi verfasserin aut Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs. adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy Daichi Kobayashi verfasserin aut Yuki Sugiura verfasserin aut Eiji Umemoto verfasserin aut Akira Takeda verfasserin aut Hisashi Ueta verfasserin aut Haruko Hayasaka verfasserin aut Shinsuke Matsuzaki verfasserin aut Shinsuke Matsuzaki verfasserin aut Tomoya Katakai verfasserin aut Makoto Suematsu verfasserin aut Itaru Hamachi verfasserin aut Gennady G. Yegutkin verfasserin aut Marko Salmi verfasserin aut Marko Salmi verfasserin aut Sirpa Jalkanen verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.786595 kostenfrei https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
spelling	10.3389/fimmu.2021.786595 doi (DE-627)DOAJ019597207 (DE-599)DOAJ97740f20ca8d49139c8d35c2edaa2c0f DE-627 ger DE-627 rakwb eng RC581-607 Daichi Kobayashi verfasserin aut Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs. adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy Daichi Kobayashi verfasserin aut Yuki Sugiura verfasserin aut Eiji Umemoto verfasserin aut Akira Takeda verfasserin aut Hisashi Ueta verfasserin aut Haruko Hayasaka verfasserin aut Shinsuke Matsuzaki verfasserin aut Shinsuke Matsuzaki verfasserin aut Tomoya Katakai verfasserin aut Makoto Suematsu verfasserin aut Itaru Hamachi verfasserin aut Gennady G. Yegutkin verfasserin aut Marko Salmi verfasserin aut Marko Salmi verfasserin aut Sirpa Jalkanen verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.786595 kostenfrei https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfields_unstemmed	10.3389/fimmu.2021.786595 doi (DE-627)DOAJ019597207 (DE-599)DOAJ97740f20ca8d49139c8d35c2edaa2c0f DE-627 ger DE-627 rakwb eng RC581-607 Daichi Kobayashi verfasserin aut Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs. adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy Daichi Kobayashi verfasserin aut Yuki Sugiura verfasserin aut Eiji Umemoto verfasserin aut Akira Takeda verfasserin aut Hisashi Ueta verfasserin aut Haruko Hayasaka verfasserin aut Shinsuke Matsuzaki verfasserin aut Shinsuke Matsuzaki verfasserin aut Tomoya Katakai verfasserin aut Makoto Suematsu verfasserin aut Itaru Hamachi verfasserin aut Gennady G. Yegutkin verfasserin aut Marko Salmi verfasserin aut Marko Salmi verfasserin aut Sirpa Jalkanen verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.786595 kostenfrei https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsGer	10.3389/fimmu.2021.786595 doi (DE-627)DOAJ019597207 (DE-599)DOAJ97740f20ca8d49139c8d35c2edaa2c0f DE-627 ger DE-627 rakwb eng RC581-607 Daichi Kobayashi verfasserin aut Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs. adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy Daichi Kobayashi verfasserin aut Yuki Sugiura verfasserin aut Eiji Umemoto verfasserin aut Akira Takeda verfasserin aut Hisashi Ueta verfasserin aut Haruko Hayasaka verfasserin aut Shinsuke Matsuzaki verfasserin aut Shinsuke Matsuzaki verfasserin aut Tomoya Katakai verfasserin aut Makoto Suematsu verfasserin aut Itaru Hamachi verfasserin aut Gennady G. Yegutkin verfasserin aut Marko Salmi verfasserin aut Marko Salmi verfasserin aut Sirpa Jalkanen verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.786595 kostenfrei https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsSound	10.3389/fimmu.2021.786595 doi (DE-627)DOAJ019597207 (DE-599)DOAJ97740f20ca8d49139c8d35c2edaa2c0f DE-627 ger DE-627 rakwb eng RC581-607 Daichi Kobayashi verfasserin aut Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs. adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy Daichi Kobayashi verfasserin aut Yuki Sugiura verfasserin aut Eiji Umemoto verfasserin aut Akira Takeda verfasserin aut Hisashi Ueta verfasserin aut Haruko Hayasaka verfasserin aut Shinsuke Matsuzaki verfasserin aut Shinsuke Matsuzaki verfasserin aut Tomoya Katakai verfasserin aut Makoto Suematsu verfasserin aut Itaru Hamachi verfasserin aut Gennady G. Yegutkin verfasserin aut Marko Salmi verfasserin aut Marko Salmi verfasserin aut Sirpa Jalkanen verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut Masayuki Miyasaka verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.786595 kostenfrei https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
language	English
source	In Frontiers in Immunology 12(2021) volume:12 year:2021
sourceStr	In Frontiers in Immunology 12(2021) volume:12 year:2021
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs) Immunologic diseases. Allergy
isfreeaccess_bool	true
container_title	Frontiers in Immunology
authorswithroles_txt_mv	Daichi Kobayashi @@aut@@ Yuki Sugiura @@aut@@ Eiji Umemoto @@aut@@ Akira Takeda @@aut@@ Hisashi Ueta @@aut@@ Haruko Hayasaka @@aut@@ Shinsuke Matsuzaki @@aut@@ Tomoya Katakai @@aut@@ Makoto Suematsu @@aut@@ Itaru Hamachi @@aut@@ Gennady G. Yegutkin @@aut@@ Marko Salmi @@aut@@ Sirpa Jalkanen @@aut@@ Masayuki Miyasaka @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	657998354
id	DOAJ019597207
language_de	englisch
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callnumber-first	R - Medicine
author	Daichi Kobayashi
spellingShingle	Daichi Kobayashi misc RC581-607 misc adenosine 5’-triphosphate (ATP) misc T cells misc chemokines misc cell migration misc lymph nodes (LNs) misc Immunologic diseases. Allergy Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes
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topic_title	RC581-607 Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes adenosine 5’-triphosphate (ATP) T cells chemokines cell migration lymph nodes (LNs)
topic	misc RC581-607 misc adenosine 5’-triphosphate (ATP) misc T cells misc chemokines misc cell migration misc lymph nodes (LNs) misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc adenosine 5’-triphosphate (ATP) misc T cells misc chemokines misc cell migration misc lymph nodes (LNs) misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc adenosine 5’-triphosphate (ATP) misc T cells misc chemokines misc cell migration misc lymph nodes (LNs) misc Immunologic diseases. Allergy
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title	Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes
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title_full	Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes
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author_browse	Daichi Kobayashi Yuki Sugiura Eiji Umemoto Akira Takeda Hisashi Ueta Haruko Hayasaka Shinsuke Matsuzaki Tomoya Katakai Makoto Suematsu Itaru Hamachi Gennady G. Yegutkin Marko Salmi Sirpa Jalkanen Masayuki Miyasaka
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doi_str_mv	10.3389/fimmu.2021.786595
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title_sort	extracellular atp limits homeostatic t cell migration within lymph nodes
callnumber	RC581-607
title_auth	Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes
abstract	Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs.
abstractGer	Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs.
abstract_unstemmed	Whereas adenosine 5’-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs.
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title_short	Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes
url	https://doi.org/10.3389/fimmu.2021.786595 https://doaj.org/article/97740f20ca8d49139c8d35c2edaa2c0f https://www.frontiersin.org/articles/10.3389/fimmu.2021.786595/full https://doaj.org/toc/1664-3224
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author2	Daichi Kobayashi Yuki Sugiura Eiji Umemoto Akira Takeda Hisashi Ueta Haruko Hayasaka Shinsuke Matsuzaki Tomoya Katakai Makoto Suematsu Itaru Hamachi Gennady G. Yegutkin Marko Salmi Sirpa Jalkanen Masayuki Miyasaka
author2Str	Daichi Kobayashi Yuki Sugiura Eiji Umemoto Akira Takeda Hisashi Ueta Haruko Hayasaka Shinsuke Matsuzaki Tomoya Katakai Makoto Suematsu Itaru Hamachi Gennady G. Yegutkin Marko Salmi Sirpa Jalkanen Masayuki Miyasaka
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up_date	2024-07-04T00:12:03.924Z
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Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes

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