Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library

Oxidized phospholipids (OxPLs) are widely held to be associated with various diseases, such as arteriosclerosis, diabetes, and cancer. To characterize the structure-specific behavior of OxPLs and their physiological relevance, we developed a comprehensive analytical method by establishing a measured...
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Autor*in:	Ryohei Aoyagi [verfasserIn] Kazutaka Ikeda [verfasserIn] Yosuke Isobe [verfasserIn] Makoto Arita [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	lipidomics lipoxygenase mass spectrometry oxidized lipids untargeted lipidomics broad-targeted lipidomics

Übergeordnetes Werk:	In: Journal of Lipid Research - Elsevier, 2021, 58(2017), 11, Seite 2229-2237
Übergeordnetes Werk:	volume:58 ; year:2017 ; number:11 ; pages:2229-2237

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1194/jlr.D077123

Katalog-ID:	DOAJ019907273

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520			\|a Oxidized phospholipids (OxPLs) are widely held to be associated with various diseases, such as arteriosclerosis, diabetes, and cancer. To characterize the structure-specific behavior of OxPLs and their physiological relevance, we developed a comprehensive analytical method by establishing a measured MS/MS spectra library of OxPLs. Biogenic OxPLs were prepared by the addition of specific oxidized fatty acids to cultured cells, where they were incorporated into cellular phospholipids, and untargeted lipidomics by LC-quadrupole/TOF-MS was applied to collect MS/MS spectra for the OxPLs. Based on the measured MS/MS spectra for about 400 molecular species of the biogenic OxPLs, we developed a broad-targeted lipidomics system using triple quadrupole MS. Separation precision of structural isomers was optimized by multiple reaction monitoring analysis and this system enabled us to detect OxPLs at levels as low as 10 fmol. When applied to biological samples, i.e., mouse peritoneal macrophages, this system enabled us to monitor a series of OxPLs endogenously produced in a 12/15-lipoxygenase-dependent manner. This advanced analytical method will be useful to elucidate the structure-specific behavior of OxPLs and their physiological relevance in vivo.
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language	English
source	In Journal of Lipid Research 58(2017), 11, Seite 2229-2237 volume:58 year:2017 number:11 pages:2229-2237
sourceStr	In Journal of Lipid Research 58(2017), 11, Seite 2229-2237 volume:58 year:2017 number:11 pages:2229-2237
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	lipidomics lipoxygenase mass spectrometry oxidized lipids untargeted lipidomics broad-targeted lipidomics Biochemistry
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container_title	Journal of Lipid Research
authorswithroles_txt_mv	Ryohei Aoyagi @@aut@@ Kazutaka Ikeda @@aut@@ Yosuke Isobe @@aut@@ Makoto Arita @@aut@@
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callnumber-first	Q - Science
author	Ryohei Aoyagi
spellingShingle	Ryohei Aoyagi misc QD415-436 misc lipidomics misc lipoxygenase misc mass spectrometry misc oxidized lipids misc untargeted lipidomics misc broad-targeted lipidomics misc Biochemistry Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library
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topic_title	QD415-436 Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library lipidomics lipoxygenase mass spectrometry oxidized lipids untargeted lipidomics broad-targeted lipidomics
topic	misc QD415-436 misc lipidomics misc lipoxygenase misc mass spectrometry misc oxidized lipids misc untargeted lipidomics misc broad-targeted lipidomics misc Biochemistry
topic_unstemmed	misc QD415-436 misc lipidomics misc lipoxygenase misc mass spectrometry misc oxidized lipids misc untargeted lipidomics misc broad-targeted lipidomics misc Biochemistry
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title	Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library
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title_full	Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library
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title_sort	comprehensive analyses of oxidized phospholipids using a measured ms/ms spectra library
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title_auth	Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library
abstract	Oxidized phospholipids (OxPLs) are widely held to be associated with various diseases, such as arteriosclerosis, diabetes, and cancer. To characterize the structure-specific behavior of OxPLs and their physiological relevance, we developed a comprehensive analytical method by establishing a measured MS/MS spectra library of OxPLs. Biogenic OxPLs were prepared by the addition of specific oxidized fatty acids to cultured cells, where they were incorporated into cellular phospholipids, and untargeted lipidomics by LC-quadrupole/TOF-MS was applied to collect MS/MS spectra for the OxPLs. Based on the measured MS/MS spectra for about 400 molecular species of the biogenic OxPLs, we developed a broad-targeted lipidomics system using triple quadrupole MS. Separation precision of structural isomers was optimized by multiple reaction monitoring analysis and this system enabled us to detect OxPLs at levels as low as 10 fmol. When applied to biological samples, i.e., mouse peritoneal macrophages, this system enabled us to monitor a series of OxPLs endogenously produced in a 12/15-lipoxygenase-dependent manner. This advanced analytical method will be useful to elucidate the structure-specific behavior of OxPLs and their physiological relevance in vivo.
abstractGer	Oxidized phospholipids (OxPLs) are widely held to be associated with various diseases, such as arteriosclerosis, diabetes, and cancer. To characterize the structure-specific behavior of OxPLs and their physiological relevance, we developed a comprehensive analytical method by establishing a measured MS/MS spectra library of OxPLs. Biogenic OxPLs were prepared by the addition of specific oxidized fatty acids to cultured cells, where they were incorporated into cellular phospholipids, and untargeted lipidomics by LC-quadrupole/TOF-MS was applied to collect MS/MS spectra for the OxPLs. Based on the measured MS/MS spectra for about 400 molecular species of the biogenic OxPLs, we developed a broad-targeted lipidomics system using triple quadrupole MS. Separation precision of structural isomers was optimized by multiple reaction monitoring analysis and this system enabled us to detect OxPLs at levels as low as 10 fmol. When applied to biological samples, i.e., mouse peritoneal macrophages, this system enabled us to monitor a series of OxPLs endogenously produced in a 12/15-lipoxygenase-dependent manner. This advanced analytical method will be useful to elucidate the structure-specific behavior of OxPLs and their physiological relevance in vivo.
abstract_unstemmed	Oxidized phospholipids (OxPLs) are widely held to be associated with various diseases, such as arteriosclerosis, diabetes, and cancer. To characterize the structure-specific behavior of OxPLs and their physiological relevance, we developed a comprehensive analytical method by establishing a measured MS/MS spectra library of OxPLs. Biogenic OxPLs were prepared by the addition of specific oxidized fatty acids to cultured cells, where they were incorporated into cellular phospholipids, and untargeted lipidomics by LC-quadrupole/TOF-MS was applied to collect MS/MS spectra for the OxPLs. Based on the measured MS/MS spectra for about 400 molecular species of the biogenic OxPLs, we developed a broad-targeted lipidomics system using triple quadrupole MS. Separation precision of structural isomers was optimized by multiple reaction monitoring analysis and this system enabled us to detect OxPLs at levels as low as 10 fmol. When applied to biological samples, i.e., mouse peritoneal macrophages, this system enabled us to monitor a series of OxPLs endogenously produced in a 12/15-lipoxygenase-dependent manner. This advanced analytical method will be useful to elucidate the structure-specific behavior of OxPLs and their physiological relevance in vivo.
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title_short	Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library
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Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library

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