Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.

The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence as...
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Autor*in:	Xiaoquan Li [verfasserIn] Peter V Lidsky [verfasserIn] Yinghong Xiao [verfasserIn] Chien-Ting Wu [verfasserIn] Miguel Garcia-Knight [verfasserIn] Junjiao Yang [verfasserIn] Tsuguhisa Nakayama [verfasserIn] Jayakar V Nayak [verfasserIn] Peter K Jackson [verfasserIn] Raul Andino [verfasserIn] Xiaokun Shu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Übergeordnetes Werk:	In: PLoS Pathogens - Public Library of Science (PLoS), 2005, 17(2021), 9, p e1009898
Übergeordnetes Werk:	volume:17 ; year:2021 ; number:9, p e1009898

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1371/journal.ppat.1009898

Katalog-ID:	DOAJ020215991

Internformat


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allfields	10.1371/journal.ppat.1009898 doi (DE-627)DOAJ020215991 (DE-599)DOAJ552d796afa0a42e6b7dcd1f4fccebbef DE-627 ger DE-627 rakwb eng RC581-607 QH301-705.5 Xiaoquan Li verfasserin aut Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2. Immunologic diseases. Allergy Biology (General) Peter V Lidsky verfasserin aut Yinghong Xiao verfasserin aut Chien-Ting Wu verfasserin aut Miguel Garcia-Knight verfasserin aut Junjiao Yang verfasserin aut Tsuguhisa Nakayama verfasserin aut Jayakar V Nayak verfasserin aut Peter K Jackson verfasserin aut Raul Andino verfasserin aut Xiaokun Shu verfasserin aut In PLoS Pathogens Public Library of Science (PLoS), 2005 17(2021), 9, p e1009898 (DE-627)501074422 (DE-600)2205412-1 15537374 nnns volume:17 year:2021 number:9, p e1009898 https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef kostenfrei https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/toc/1553-7366 Journal toc kostenfrei https://doaj.org/toc/1553-7374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2021 9, p e1009898
spelling	10.1371/journal.ppat.1009898 doi (DE-627)DOAJ020215991 (DE-599)DOAJ552d796afa0a42e6b7dcd1f4fccebbef DE-627 ger DE-627 rakwb eng RC581-607 QH301-705.5 Xiaoquan Li verfasserin aut Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2. Immunologic diseases. Allergy Biology (General) Peter V Lidsky verfasserin aut Yinghong Xiao verfasserin aut Chien-Ting Wu verfasserin aut Miguel Garcia-Knight verfasserin aut Junjiao Yang verfasserin aut Tsuguhisa Nakayama verfasserin aut Jayakar V Nayak verfasserin aut Peter K Jackson verfasserin aut Raul Andino verfasserin aut Xiaokun Shu verfasserin aut In PLoS Pathogens Public Library of Science (PLoS), 2005 17(2021), 9, p e1009898 (DE-627)501074422 (DE-600)2205412-1 15537374 nnns volume:17 year:2021 number:9, p e1009898 https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef kostenfrei https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/toc/1553-7366 Journal toc kostenfrei https://doaj.org/toc/1553-7374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2021 9, p e1009898
allfields_unstemmed	10.1371/journal.ppat.1009898 doi (DE-627)DOAJ020215991 (DE-599)DOAJ552d796afa0a42e6b7dcd1f4fccebbef DE-627 ger DE-627 rakwb eng RC581-607 QH301-705.5 Xiaoquan Li verfasserin aut Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2. Immunologic diseases. Allergy Biology (General) Peter V Lidsky verfasserin aut Yinghong Xiao verfasserin aut Chien-Ting Wu verfasserin aut Miguel Garcia-Knight verfasserin aut Junjiao Yang verfasserin aut Tsuguhisa Nakayama verfasserin aut Jayakar V Nayak verfasserin aut Peter K Jackson verfasserin aut Raul Andino verfasserin aut Xiaokun Shu verfasserin aut In PLoS Pathogens Public Library of Science (PLoS), 2005 17(2021), 9, p e1009898 (DE-627)501074422 (DE-600)2205412-1 15537374 nnns volume:17 year:2021 number:9, p e1009898 https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef kostenfrei https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/toc/1553-7366 Journal toc kostenfrei https://doaj.org/toc/1553-7374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2021 9, p e1009898
allfieldsGer	10.1371/journal.ppat.1009898 doi (DE-627)DOAJ020215991 (DE-599)DOAJ552d796afa0a42e6b7dcd1f4fccebbef DE-627 ger DE-627 rakwb eng RC581-607 QH301-705.5 Xiaoquan Li verfasserin aut Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2. Immunologic diseases. Allergy Biology (General) Peter V Lidsky verfasserin aut Yinghong Xiao verfasserin aut Chien-Ting Wu verfasserin aut Miguel Garcia-Knight verfasserin aut Junjiao Yang verfasserin aut Tsuguhisa Nakayama verfasserin aut Jayakar V Nayak verfasserin aut Peter K Jackson verfasserin aut Raul Andino verfasserin aut Xiaokun Shu verfasserin aut In PLoS Pathogens Public Library of Science (PLoS), 2005 17(2021), 9, p e1009898 (DE-627)501074422 (DE-600)2205412-1 15537374 nnns volume:17 year:2021 number:9, p e1009898 https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef kostenfrei https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/toc/1553-7366 Journal toc kostenfrei https://doaj.org/toc/1553-7374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2021 9, p e1009898
allfieldsSound	10.1371/journal.ppat.1009898 doi (DE-627)DOAJ020215991 (DE-599)DOAJ552d796afa0a42e6b7dcd1f4fccebbef DE-627 ger DE-627 rakwb eng RC581-607 QH301-705.5 Xiaoquan Li verfasserin aut Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2. Immunologic diseases. Allergy Biology (General) Peter V Lidsky verfasserin aut Yinghong Xiao verfasserin aut Chien-Ting Wu verfasserin aut Miguel Garcia-Knight verfasserin aut Junjiao Yang verfasserin aut Tsuguhisa Nakayama verfasserin aut Jayakar V Nayak verfasserin aut Peter K Jackson verfasserin aut Raul Andino verfasserin aut Xiaokun Shu verfasserin aut In PLoS Pathogens Public Library of Science (PLoS), 2005 17(2021), 9, p e1009898 (DE-627)501074422 (DE-600)2205412-1 15537374 nnns volume:17 year:2021 number:9, p e1009898 https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef kostenfrei https://doi.org/10.1371/journal.ppat.1009898 kostenfrei https://doaj.org/toc/1553-7366 Journal toc kostenfrei https://doaj.org/toc/1553-7374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2021 9, p e1009898
language	English
source	In PLoS Pathogens 17(2021), 9, p e1009898 volume:17 year:2021 number:9, p e1009898
sourceStr	In PLoS Pathogens 17(2021), 9, p e1009898 volume:17 year:2021 number:9, p e1009898
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Immunologic diseases. Allergy Biology (General)
isfreeaccess_bool	true
container_title	PLoS Pathogens
authorswithroles_txt_mv	Xiaoquan Li @@aut@@ Peter V Lidsky @@aut@@ Yinghong Xiao @@aut@@ Chien-Ting Wu @@aut@@ Miguel Garcia-Knight @@aut@@ Junjiao Yang @@aut@@ Tsuguhisa Nakayama @@aut@@ Jayakar V Nayak @@aut@@ Peter K Jackson @@aut@@ Raul Andino @@aut@@ Xiaokun Shu @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	501074422
id	DOAJ020215991
language_de	englisch
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callnumber-first	R - Medicine
author	Xiaoquan Li
spellingShingle	Xiaoquan Li misc RC581-607 misc QH301-705.5 misc Immunologic diseases. Allergy misc Biology (General) Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.
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topic_title	RC581-607 QH301-705.5 Ethacridine inhibits SARS-CoV-2 by inactivating viral particles
topic	misc RC581-607 misc QH301-705.5 misc Immunologic diseases. Allergy misc Biology (General)
topic_unstemmed	misc RC581-607 misc QH301-705.5 misc Immunologic diseases. Allergy misc Biology (General)
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title	Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.
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title_full	Ethacridine inhibits SARS-CoV-2 by inactivating viral particles
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title_sort	ethacridine inhibits sars-cov-2 by inactivating viral particles
callnumber	RC581-607
title_auth	Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.
abstract	The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2.
abstractGer	The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2.
abstract_unstemmed	The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report the discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening of an FDA-approved drug library in living cells using a fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages of viral infection demonstrate that the main mode of action of ethacridine is through inactivation of viral particles, preventing their binding to the host cells. Consistently, ethacridine is effective in various cell types, including primary human nasal epithelial cells that are cultured in an air-liquid interface. Taken together, our work identifies a promising, potent, and new use of the old drug via a distinct mode of action for inhibiting SARS-CoV-2.
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container_issue	9, p e1009898
title_short	Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.
url	https://doi.org/10.1371/journal.ppat.1009898 https://doaj.org/article/552d796afa0a42e6b7dcd1f4fccebbef https://doaj.org/toc/1553-7366 https://doaj.org/toc/1553-7374
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doi_str	10.1371/journal.ppat.1009898
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up_date	2024-07-03T13:39:52.522Z
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Ethacridine inhibits SARS-CoV-2 by inactivating viral particles.

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