SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns

SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Elena Vazquez-Alejo [verfasserIn] Laura Tarancon-Diez [verfasserIn] Itzíar Carrasco [verfasserIn] Sara Vigil-Vázquez [verfasserIn] Mar Muñoz-Chapuli [verfasserIn] Elena Rincón-López [verfasserIn] Jesús Saavedra-Lozano [verfasserIn] Mar Santos-Sebastián [verfasserIn] David Aguilera-Alonso [verfasserIn] Alicia Hernanz-Lobo [verfasserIn] Begoña Santiago-García [verfasserIn] Juan Antonio de León-Luis [verfasserIn] Patricia Muñoz [verfasserIn] Manuel Sánchez-Luna [verfasserIn] María Luisa Navarro [verfasserIn] Mª Ángeles Muñoz-Fernández [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 13(2022)
Übergeordnetes Werk:	volume:13 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2022.947549

Katalog-ID:	DOAJ020234759

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allfields	10.3389/fimmu.2022.947549 doi (DE-627)DOAJ020234759 (DE-599)DOAJ660b57f9f1c046e58310142aafd64e2f DE-627 ger DE-627 rakwb eng RC581-607 Elena Vazquez-Alejo verfasserin aut SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy Laura Tarancon-Diez verfasserin aut Itzíar Carrasco verfasserin aut Itzíar Carrasco verfasserin aut Sara Vigil-Vázquez verfasserin aut Mar Muñoz-Chapuli verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Juan Antonio de León-Luis verfasserin aut Juan Antonio de León-Luis verfasserin aut Patricia Muñoz verfasserin aut Manuel Sánchez-Luna verfasserin aut Manuel Sánchez-Luna verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut Mª Ángeles Muñoz-Fernández verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 13(2022) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:13 year:2022 https://doi.org/10.3389/fimmu.2022.947549 kostenfrei https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
spelling	10.3389/fimmu.2022.947549 doi (DE-627)DOAJ020234759 (DE-599)DOAJ660b57f9f1c046e58310142aafd64e2f DE-627 ger DE-627 rakwb eng RC581-607 Elena Vazquez-Alejo verfasserin aut SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy Laura Tarancon-Diez verfasserin aut Itzíar Carrasco verfasserin aut Itzíar Carrasco verfasserin aut Sara Vigil-Vázquez verfasserin aut Mar Muñoz-Chapuli verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Juan Antonio de León-Luis verfasserin aut Juan Antonio de León-Luis verfasserin aut Patricia Muñoz verfasserin aut Manuel Sánchez-Luna verfasserin aut Manuel Sánchez-Luna verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut Mª Ángeles Muñoz-Fernández verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 13(2022) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:13 year:2022 https://doi.org/10.3389/fimmu.2022.947549 kostenfrei https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfields_unstemmed	10.3389/fimmu.2022.947549 doi (DE-627)DOAJ020234759 (DE-599)DOAJ660b57f9f1c046e58310142aafd64e2f DE-627 ger DE-627 rakwb eng RC581-607 Elena Vazquez-Alejo verfasserin aut SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy Laura Tarancon-Diez verfasserin aut Itzíar Carrasco verfasserin aut Itzíar Carrasco verfasserin aut Sara Vigil-Vázquez verfasserin aut Mar Muñoz-Chapuli verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Juan Antonio de León-Luis verfasserin aut Juan Antonio de León-Luis verfasserin aut Patricia Muñoz verfasserin aut Manuel Sánchez-Luna verfasserin aut Manuel Sánchez-Luna verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut Mª Ángeles Muñoz-Fernández verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 13(2022) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:13 year:2022 https://doi.org/10.3389/fimmu.2022.947549 kostenfrei https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsGer	10.3389/fimmu.2022.947549 doi (DE-627)DOAJ020234759 (DE-599)DOAJ660b57f9f1c046e58310142aafd64e2f DE-627 ger DE-627 rakwb eng RC581-607 Elena Vazquez-Alejo verfasserin aut SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy Laura Tarancon-Diez verfasserin aut Itzíar Carrasco verfasserin aut Itzíar Carrasco verfasserin aut Sara Vigil-Vázquez verfasserin aut Mar Muñoz-Chapuli verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Juan Antonio de León-Luis verfasserin aut Juan Antonio de León-Luis verfasserin aut Patricia Muñoz verfasserin aut Manuel Sánchez-Luna verfasserin aut Manuel Sánchez-Luna verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut Mª Ángeles Muñoz-Fernández verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 13(2022) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:13 year:2022 https://doi.org/10.3389/fimmu.2022.947549 kostenfrei https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsSound	10.3389/fimmu.2022.947549 doi (DE-627)DOAJ020234759 (DE-599)DOAJ660b57f9f1c046e58310142aafd64e2f DE-627 ger DE-627 rakwb eng RC581-607 Elena Vazquez-Alejo verfasserin aut SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months. SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy Laura Tarancon-Diez verfasserin aut Itzíar Carrasco verfasserin aut Itzíar Carrasco verfasserin aut Sara Vigil-Vázquez verfasserin aut Mar Muñoz-Chapuli verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Elena Rincón-López verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Jesús Saavedra-Lozano verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut Mar Santos-Sebastián verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut David Aguilera-Alonso verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Alicia Hernanz-Lobo verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Begoña Santiago-García verfasserin aut Juan Antonio de León-Luis verfasserin aut Juan Antonio de León-Luis verfasserin aut Patricia Muñoz verfasserin aut Manuel Sánchez-Luna verfasserin aut Manuel Sánchez-Luna verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut María Luisa Navarro verfasserin aut Mª Ángeles Muñoz-Fernández verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 13(2022) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:13 year:2022 https://doi.org/10.3389/fimmu.2022.947549 kostenfrei https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
language	English
source	In Frontiers in Immunology 13(2022) volume:13 year:2022
sourceStr	In Frontiers in Immunology 13(2022) volume:13 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis Immunologic diseases. Allergy
isfreeaccess_bool	true
container_title	Frontiers in Immunology
authorswithroles_txt_mv	Elena Vazquez-Alejo @@aut@@ Laura Tarancon-Diez @@aut@@ Itzíar Carrasco @@aut@@ Sara Vigil-Vázquez @@aut@@ Mar Muñoz-Chapuli @@aut@@ Elena Rincón-López @@aut@@ Jesús Saavedra-Lozano @@aut@@ Mar Santos-Sebastián @@aut@@ David Aguilera-Alonso @@aut@@ Alicia Hernanz-Lobo @@aut@@ Begoña Santiago-García @@aut@@ Juan Antonio de León-Luis @@aut@@ Patricia Muñoz @@aut@@ Manuel Sánchez-Luna @@aut@@ María Luisa Navarro @@aut@@ Mª Ángeles Muñoz-Fernández @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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callnumber-first	R - Medicine
author	Elena Vazquez-Alejo
spellingShingle	Elena Vazquez-Alejo misc RC581-607 misc SARS-CoV2 misc pregnancy misc SARS-CoV2 exposed newborns misc immune system misc longitudinal analysis misc Immunologic diseases. Allergy SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns
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topic_title	RC581-607 SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns SARS-CoV2 pregnancy SARS-CoV2 exposed newborns immune system longitudinal analysis
topic	misc RC581-607 misc SARS-CoV2 misc pregnancy misc SARS-CoV2 exposed newborns misc immune system misc longitudinal analysis misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc SARS-CoV2 misc pregnancy misc SARS-CoV2 exposed newborns misc immune system misc longitudinal analysis misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc SARS-CoV2 misc pregnancy misc SARS-CoV2 exposed newborns misc immune system misc longitudinal analysis misc Immunologic diseases. Allergy
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title	SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns
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title_full	SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns
author_sort	Elena Vazquez-Alejo
journal	Frontiers in Immunology
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author_browse	Elena Vazquez-Alejo Laura Tarancon-Diez Itzíar Carrasco Sara Vigil-Vázquez Mar Muñoz-Chapuli Elena Rincón-López Jesús Saavedra-Lozano Mar Santos-Sebastián David Aguilera-Alonso Alicia Hernanz-Lobo Begoña Santiago-García Juan Antonio de León-Luis Patricia Muñoz Manuel Sánchez-Luna María Luisa Navarro Mª Ángeles Muñoz-Fernández
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author-letter	Elena Vazquez-Alejo
doi_str_mv	10.3389/fimmu.2022.947549
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title_sort	sars-cov2 infection during pregnancy causes persistent immune abnormalities in women without affecting the newborns
callnumber	RC581-607
title_auth	SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns
abstract	SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months.
abstractGer	SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months.
abstract_unstemmed	SARS-CoV2 infection in pregnancy and exposed newborns is poorly known. We performed a longitudinal analysis of immune system and determined soluble cytokine levels in pregnant women infected with SARS-CoV2 and in their newborns. Women with confirmed SARS-CoV2 infection and their exposed uninfected newborns were recruited from Hospital General Universitario Gregorio Marañón. Peripheral blood mononuclear cells (PBMCs), cord cells and plasma were collected at birth and 6 months later. Immunophenotyping of natural killer (NK), monocytes and CD4/CD8 T-cells were studied in cryopreserved PBMCs and cord cells by multiparametric flow cytometry. Up to 4 soluble pro/anti-inflammatory cytokines were assessed in plasma/cord plasma by ELISA assay. SARS-CoV2-infected mothers and their newborns were compared to matched healthy non-SARS-CoV2-infected mothers and their newborns. The TNFα and IL-10 levels of infected mothers were higher at baseline than those of healthy controls. Infected mothers showed increased NK cells activation and reduced expression of maturation markers that reverted after 6 months. They also had high levels of Central Memory and low Effector Memory CD4-T cell subsets. Additionally, the increased CD4- and CD8-T cell activation (CD154 and CD38) and exhaustion (TIM3/TIGIT) levels at baseline compared to controls remained elevated after 6 months. Regarding Treg cells, the levels were lower at infected mothers at baseline but reverted after 6 months. No newborn was infected at birth. The lower levels of monocytes, NK and CD4-T cells observed at SARS-CoV2-exposed newborns compared to unexposed controls significantly increased 6 months later. In conclusion, SARS-CoV2 infection during pregnancy shows differences in immunological components that could lead newborns to future clinical implications after birth. However, SARS-CoV2 exposed 6-months-old newborns showed no immune misbalance, whereas the infected mothers maintain increased activation and exhaustion levels in T-cells after 6 months.
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title_short	SARS-CoV2 Infection During Pregnancy Causes Persistent Immune Abnormalities in Women Without Affecting the Newborns
url	https://doi.org/10.3389/fimmu.2022.947549 https://doaj.org/article/660b57f9f1c046e58310142aafd64e2f https://www.frontiersin.org/articles/10.3389/fimmu.2022.947549/full https://doaj.org/toc/1664-3224
remote_bool	true
author2	Laura Tarancon-Diez Itzíar Carrasco Sara Vigil-Vázquez Mar Muñoz-Chapuli Elena Rincón-López Jesús Saavedra-Lozano Mar Santos-Sebastián David Aguilera-Alonso Alicia Hernanz-Lobo Begoña Santiago-García Juan Antonio de León-Luis Patricia Muñoz Manuel Sánchez-Luna María Luisa Navarro Mª Ángeles Muñoz-Fernández
author2Str	Laura Tarancon-Diez Itzíar Carrasco Sara Vigil-Vázquez Mar Muñoz-Chapuli Elena Rincón-López Jesús Saavedra-Lozano Mar Santos-Sebastián David Aguilera-Alonso Alicia Hernanz-Lobo Begoña Santiago-García Juan Antonio de León-Luis Patricia Muñoz Manuel Sánchez-Luna María Luisa Navarro Mª Ángeles Muñoz-Fernández
ppnlink	657998354
callnumber-subject	RC - Internal Medicine
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3389/fimmu.2022.947549
callnumber-a	RC581-607
up_date	2024-07-03T13:46:23.779Z
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