Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on th...
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Autor*in:	César Gómez-Hernández [verfasserIn] Karine Rezende-Oliveira [verfasserIn] Gabriel Antônio Nogueira Nascentes [verfasserIn] Lara Rocha Batista [verfasserIn] Henrique Borges Kappel [verfasserIn] José Alejandro Martinez-Ibarra [verfasserIn] Francisco Trujillo Contreras [verfasserIn] Eliane Lages-Silva [verfasserIn] Luis Eduardo Ramírez [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Trypanosoma cruzi México Caracterização biológica Caracterização genética

Übergeordnetes Werk:	In: Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT), 2004, 44(2011), 6, Seite 684-690
Übergeordnetes Werk:	volume:44 ; year:2011 ; number:6 ; pages:684-690

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/s0037-86822011005000058

Katalog-ID:	DOAJ020874758

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520			\|a INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.
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allfields	10.1590/s0037-86822011005000058 doi (DE-627)DOAJ020874758 (DE-599)DOAJ0f88a21a0b844cb3b30dc0fd57065e2f DE-627 ger DE-627 rakwb eng RC955-962 César Gómez-Hernández verfasserin aut Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine Karine Rezende-Oliveira verfasserin aut Gabriel Antônio Nogueira Nascentes verfasserin aut Lara Rocha Batista verfasserin aut Henrique Borges Kappel verfasserin aut José Alejandro Martinez-Ibarra verfasserin aut Francisco Trujillo Contreras verfasserin aut Eliane Lages-Silva verfasserin aut Luis Eduardo Ramírez verfasserin aut In Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT), 2004 44(2011), 6, Seite 684-690 (DE-627)324614918 (DE-600)2028921-2 16789849 nnns volume:44 year:2011 number:6 pages:684-690 https://doi.org/10.1590/s0037-86822011005000058 kostenfrei https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-9849 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2011 6 684-690
spelling	10.1590/s0037-86822011005000058 doi (DE-627)DOAJ020874758 (DE-599)DOAJ0f88a21a0b844cb3b30dc0fd57065e2f DE-627 ger DE-627 rakwb eng RC955-962 César Gómez-Hernández verfasserin aut Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine Karine Rezende-Oliveira verfasserin aut Gabriel Antônio Nogueira Nascentes verfasserin aut Lara Rocha Batista verfasserin aut Henrique Borges Kappel verfasserin aut José Alejandro Martinez-Ibarra verfasserin aut Francisco Trujillo Contreras verfasserin aut Eliane Lages-Silva verfasserin aut Luis Eduardo Ramírez verfasserin aut In Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT), 2004 44(2011), 6, Seite 684-690 (DE-627)324614918 (DE-600)2028921-2 16789849 nnns volume:44 year:2011 number:6 pages:684-690 https://doi.org/10.1590/s0037-86822011005000058 kostenfrei https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-9849 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2011 6 684-690
allfields_unstemmed	10.1590/s0037-86822011005000058 doi (DE-627)DOAJ020874758 (DE-599)DOAJ0f88a21a0b844cb3b30dc0fd57065e2f DE-627 ger DE-627 rakwb eng RC955-962 César Gómez-Hernández verfasserin aut Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine Karine Rezende-Oliveira verfasserin aut Gabriel Antônio Nogueira Nascentes verfasserin aut Lara Rocha Batista verfasserin aut Henrique Borges Kappel verfasserin aut José Alejandro Martinez-Ibarra verfasserin aut Francisco Trujillo Contreras verfasserin aut Eliane Lages-Silva verfasserin aut Luis Eduardo Ramírez verfasserin aut In Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT), 2004 44(2011), 6, Seite 684-690 (DE-627)324614918 (DE-600)2028921-2 16789849 nnns volume:44 year:2011 number:6 pages:684-690 https://doi.org/10.1590/s0037-86822011005000058 kostenfrei https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-9849 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2011 6 684-690
allfieldsGer	10.1590/s0037-86822011005000058 doi (DE-627)DOAJ020874758 (DE-599)DOAJ0f88a21a0b844cb3b30dc0fd57065e2f DE-627 ger DE-627 rakwb eng RC955-962 César Gómez-Hernández verfasserin aut Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine Karine Rezende-Oliveira verfasserin aut Gabriel Antônio Nogueira Nascentes verfasserin aut Lara Rocha Batista verfasserin aut Henrique Borges Kappel verfasserin aut José Alejandro Martinez-Ibarra verfasserin aut Francisco Trujillo Contreras verfasserin aut Eliane Lages-Silva verfasserin aut Luis Eduardo Ramírez verfasserin aut In Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT), 2004 44(2011), 6, Seite 684-690 (DE-627)324614918 (DE-600)2028921-2 16789849 nnns volume:44 year:2011 number:6 pages:684-690 https://doi.org/10.1590/s0037-86822011005000058 kostenfrei https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-9849 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2011 6 684-690
allfieldsSound	10.1590/s0037-86822011005000058 doi (DE-627)DOAJ020874758 (DE-599)DOAJ0f88a21a0b844cb3b30dc0fd57065e2f DE-627 ger DE-627 rakwb eng RC955-962 César Gómez-Hernández verfasserin aut Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine Karine Rezende-Oliveira verfasserin aut Gabriel Antônio Nogueira Nascentes verfasserin aut Lara Rocha Batista verfasserin aut Henrique Borges Kappel verfasserin aut José Alejandro Martinez-Ibarra verfasserin aut Francisco Trujillo Contreras verfasserin aut Eliane Lages-Silva verfasserin aut Luis Eduardo Ramírez verfasserin aut In Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT), 2004 44(2011), 6, Seite 684-690 (DE-627)324614918 (DE-600)2028921-2 16789849 nnns volume:44 year:2011 number:6 pages:684-690 https://doi.org/10.1590/s0037-86822011005000058 kostenfrei https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-9849 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2011 6 684-690
language	English
source	In Revista da Sociedade Brasileira de Medicina Tropical 44(2011), 6, Seite 684-690 volume:44 year:2011 number:6 pages:684-690
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topic_facet	Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine
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publishDateDaySort_date	2011-01-01T00:00:00Z
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spellingShingle	César Gómez-Hernández misc RC955-962 misc Trypanosoma cruzi misc México misc Caracterização biológica misc Caracterização genética misc Arctic medicine. Tropical medicine Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
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issn	16789849
topic_title	RC955-962 Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Trypanosoma cruzi México Caracterização biológica Caracterização genética
topic	misc RC955-962 misc Trypanosoma cruzi misc México misc Caracterização biológica misc Caracterização genética misc Arctic medicine. Tropical medicine
topic_unstemmed	misc RC955-962 misc Trypanosoma cruzi misc México misc Caracterização biológica misc Caracterização genética misc Arctic medicine. Tropical medicine
topic_browse	misc RC955-962 misc Trypanosoma cruzi misc México misc Caracterização biológica misc Caracterização genética misc Arctic medicine. Tropical medicine
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title	Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
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title_full	Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
author_sort	César Gómez-Hernández
journal	Revista da Sociedade Brasileira de Medicina Tropical
journalStr	Revista da Sociedade Brasileira de Medicina Tropical
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author_browse	César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez
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class	RC955-962
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author-letter	César Gómez-Hernández
doi_str_mv	10.1590/s0037-86822011005000058
author2-role	verfasserin
title_sort	molecular characterization of trypanosoma cruzi mexican strains and their behavior in the mouse experimental model
callnumber	RC955-962
title_auth	Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
abstract	INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.
abstractGer	INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.
abstract_unstemmed	INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.
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container_issue	6
title_short	Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
url	https://doi.org/10.1590/s0037-86822011005000058 https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en https://doaj.org/toc/1678-9849
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author2	Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez
author2Str	Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez
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doi_str	10.1590/s0037-86822011005000058
callnumber-a	RC955-962
up_date	2024-07-03T17:31:15.419Z
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