Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility

MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes...
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Autor*in:	Qiang Ding [verfasserIn] Xiuhu Ding [verfasserIn] Shuwen Xia [verfasserIn] Fang Zhao [verfasserIn] Kunlin Chen [verfasserIn] Yong Qian [verfasserIn] Shaoxian Cao [verfasserIn] Zhiping Lin [verfasserIn] Yundong Gao [verfasserIn] Huili Wang [verfasserIn] Jifeng Zhong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	sperm motility bta-miR-6531 calcium signaling pathway SNP

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 13(2022), 10, p 1788
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:10, p 1788

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes13101788

Katalog-ID:	DOAJ020937016

Internformat


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520			\|a MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa.
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allfields	10.3390/genes13101788 doi (DE-627)DOAJ020937016 (DE-599)DOAJ061a63c06f8e4f5baa36a30e1314e126 DE-627 ger DE-627 rakwb eng QH426-470 Qiang Ding verfasserin aut Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa. sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics Xiuhu Ding verfasserin aut Shuwen Xia verfasserin aut Fang Zhao verfasserin aut Kunlin Chen verfasserin aut Yong Qian verfasserin aut Shaoxian Cao verfasserin aut Zhiping Lin verfasserin aut Yundong Gao verfasserin aut Huili Wang verfasserin aut Jifeng Zhong verfasserin aut In Genes MDPI AG, 2010 13(2022), 10, p 1788 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:10, p 1788 https://doi.org/10.3390/genes13101788 kostenfrei https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 kostenfrei https://www.mdpi.com/2073-4425/13/10/1788 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 10, p 1788
spelling	10.3390/genes13101788 doi (DE-627)DOAJ020937016 (DE-599)DOAJ061a63c06f8e4f5baa36a30e1314e126 DE-627 ger DE-627 rakwb eng QH426-470 Qiang Ding verfasserin aut Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa. sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics Xiuhu Ding verfasserin aut Shuwen Xia verfasserin aut Fang Zhao verfasserin aut Kunlin Chen verfasserin aut Yong Qian verfasserin aut Shaoxian Cao verfasserin aut Zhiping Lin verfasserin aut Yundong Gao verfasserin aut Huili Wang verfasserin aut Jifeng Zhong verfasserin aut In Genes MDPI AG, 2010 13(2022), 10, p 1788 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:10, p 1788 https://doi.org/10.3390/genes13101788 kostenfrei https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 kostenfrei https://www.mdpi.com/2073-4425/13/10/1788 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 10, p 1788
allfields_unstemmed	10.3390/genes13101788 doi (DE-627)DOAJ020937016 (DE-599)DOAJ061a63c06f8e4f5baa36a30e1314e126 DE-627 ger DE-627 rakwb eng QH426-470 Qiang Ding verfasserin aut Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa. sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics Xiuhu Ding verfasserin aut Shuwen Xia verfasserin aut Fang Zhao verfasserin aut Kunlin Chen verfasserin aut Yong Qian verfasserin aut Shaoxian Cao verfasserin aut Zhiping Lin verfasserin aut Yundong Gao verfasserin aut Huili Wang verfasserin aut Jifeng Zhong verfasserin aut In Genes MDPI AG, 2010 13(2022), 10, p 1788 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:10, p 1788 https://doi.org/10.3390/genes13101788 kostenfrei https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 kostenfrei https://www.mdpi.com/2073-4425/13/10/1788 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 10, p 1788
allfieldsGer	10.3390/genes13101788 doi (DE-627)DOAJ020937016 (DE-599)DOAJ061a63c06f8e4f5baa36a30e1314e126 DE-627 ger DE-627 rakwb eng QH426-470 Qiang Ding verfasserin aut Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa. sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics Xiuhu Ding verfasserin aut Shuwen Xia verfasserin aut Fang Zhao verfasserin aut Kunlin Chen verfasserin aut Yong Qian verfasserin aut Shaoxian Cao verfasserin aut Zhiping Lin verfasserin aut Yundong Gao verfasserin aut Huili Wang verfasserin aut Jifeng Zhong verfasserin aut In Genes MDPI AG, 2010 13(2022), 10, p 1788 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:10, p 1788 https://doi.org/10.3390/genes13101788 kostenfrei https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 kostenfrei https://www.mdpi.com/2073-4425/13/10/1788 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 10, p 1788
allfieldsSound	10.3390/genes13101788 doi (DE-627)DOAJ020937016 (DE-599)DOAJ061a63c06f8e4f5baa36a30e1314e126 DE-627 ger DE-627 rakwb eng QH426-470 Qiang Ding verfasserin aut Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa. sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics Xiuhu Ding verfasserin aut Shuwen Xia verfasserin aut Fang Zhao verfasserin aut Kunlin Chen verfasserin aut Yong Qian verfasserin aut Shaoxian Cao verfasserin aut Zhiping Lin verfasserin aut Yundong Gao verfasserin aut Huili Wang verfasserin aut Jifeng Zhong verfasserin aut In Genes MDPI AG, 2010 13(2022), 10, p 1788 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:10, p 1788 https://doi.org/10.3390/genes13101788 kostenfrei https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 kostenfrei https://www.mdpi.com/2073-4425/13/10/1788 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 10, p 1788
language	English
source	In Genes 13(2022), 10, p 1788 volume:13 year:2022 number:10, p 1788
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topic_facet	sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP Genetics
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authorswithroles_txt_mv	Qiang Ding @@aut@@ Xiuhu Ding @@aut@@ Shuwen Xia @@aut@@ Fang Zhao @@aut@@ Kunlin Chen @@aut@@ Yong Qian @@aut@@ Shaoxian Cao @@aut@@ Zhiping Lin @@aut@@ Yundong Gao @@aut@@ Huili Wang @@aut@@ Jifeng Zhong @@aut@@
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callnumber-first	Q - Science
author	Qiang Ding
spellingShingle	Qiang Ding misc QH426-470 misc sperm motility misc bta-miR-6531 misc <i<ATP2A2</i< misc calcium signaling pathway misc SNP misc Genetics Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility
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topic_title	QH426-470 Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility sperm motility bta-miR-6531 <i<ATP2A2</i< calcium signaling pathway SNP
topic	misc QH426-470 misc sperm motility misc bta-miR-6531 misc <i<ATP2A2</i< misc calcium signaling pathway misc SNP misc Genetics
topic_unstemmed	misc QH426-470 misc sperm motility misc bta-miR-6531 misc <i<ATP2A2</i< misc calcium signaling pathway misc SNP misc Genetics
topic_browse	misc QH426-470 misc sperm motility misc bta-miR-6531 misc <i<ATP2A2</i< misc calcium signaling pathway misc SNP misc Genetics
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title	Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility
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title_full	Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility
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author_browse	Qiang Ding Xiuhu Ding Shuwen Xia Fang Zhao Kunlin Chen Yong Qian Shaoxian Cao Zhiping Lin Yundong Gao Huili Wang Jifeng Zhong
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title_sort	bta-mir-6531 regulates calcium influx in bovine leydig cells and is associated with sperm motility
callnumber	QH426-470
title_auth	Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility
abstract	MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa.
abstractGer	MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa.
abstract_unstemmed	MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting <i<ATP2A2</i< of the calcium signaling pathway in bovine spermatozoa.
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container_issue	10, p 1788
title_short	Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility
url	https://doi.org/10.3390/genes13101788 https://doaj.org/article/061a63c06f8e4f5baa36a30e1314e126 https://www.mdpi.com/2073-4425/13/10/1788 https://doaj.org/toc/2073-4425
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Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted <i<ATP2A2</i< mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining <i<ATP2A2</i< expression. Moreover, we observed high calcium concentrations and high <i<ATP2A2</i< protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. 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Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility

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