Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA

<p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Arora Pankaj [verfasserIn] Sharma Ashutosh [verfasserIn] Mehta Richa [verfasserIn] Shenoy Belle [verfasserIn] Srivastava Alok [verfasserIn] Singh Vijay [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm

Übergeordnetes Werk:	In: Microbial Cell Factories - BMC, 2003, 11(2012), 1, p 150
Übergeordnetes Werk:	volume:11 ; year:2012 ; number:1, p 150

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1475-2859-11-150

Katalog-ID:	DOAJ021592241

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allfields	10.1186/1475-2859-11-150 doi (DE-627)DOAJ021592241 (DE-599)DOAJd24c133271384827a683cc1b1c7c6875 DE-627 ger DE-627 rakwb eng QR1-502 Arora Pankaj verfasserin aut Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology Sharma Ashutosh verfasserin aut Mehta Richa verfasserin aut Shenoy Belle verfasserin aut Srivastava Alok verfasserin aut Singh Vijay verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 150 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 150 https://doi.org/10.1186/1475-2859-11-150 kostenfrei https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 kostenfrei http://www.microbialcellfactories.com/content/11/1/150 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 150
spelling	10.1186/1475-2859-11-150 doi (DE-627)DOAJ021592241 (DE-599)DOAJd24c133271384827a683cc1b1c7c6875 DE-627 ger DE-627 rakwb eng QR1-502 Arora Pankaj verfasserin aut Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology Sharma Ashutosh verfasserin aut Mehta Richa verfasserin aut Shenoy Belle verfasserin aut Srivastava Alok verfasserin aut Singh Vijay verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 150 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 150 https://doi.org/10.1186/1475-2859-11-150 kostenfrei https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 kostenfrei http://www.microbialcellfactories.com/content/11/1/150 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 150
allfields_unstemmed	10.1186/1475-2859-11-150 doi (DE-627)DOAJ021592241 (DE-599)DOAJd24c133271384827a683cc1b1c7c6875 DE-627 ger DE-627 rakwb eng QR1-502 Arora Pankaj verfasserin aut Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology Sharma Ashutosh verfasserin aut Mehta Richa verfasserin aut Shenoy Belle verfasserin aut Srivastava Alok verfasserin aut Singh Vijay verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 150 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 150 https://doi.org/10.1186/1475-2859-11-150 kostenfrei https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 kostenfrei http://www.microbialcellfactories.com/content/11/1/150 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 150
allfieldsGer	10.1186/1475-2859-11-150 doi (DE-627)DOAJ021592241 (DE-599)DOAJd24c133271384827a683cc1b1c7c6875 DE-627 ger DE-627 rakwb eng QR1-502 Arora Pankaj verfasserin aut Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology Sharma Ashutosh verfasserin aut Mehta Richa verfasserin aut Shenoy Belle verfasserin aut Srivastava Alok verfasserin aut Singh Vijay verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 150 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 150 https://doi.org/10.1186/1475-2859-11-150 kostenfrei https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 kostenfrei http://www.microbialcellfactories.com/content/11/1/150 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 150
allfieldsSound	10.1186/1475-2859-11-150 doi (DE-627)DOAJ021592241 (DE-599)DOAJd24c133271384827a683cc1b1c7c6875 DE-627 ger DE-627 rakwb eng QR1-502 Arora Pankaj verfasserin aut Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p< 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology Sharma Ashutosh verfasserin aut Mehta Richa verfasserin aut Shenoy Belle verfasserin aut Srivastava Alok verfasserin aut Singh Vijay verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 150 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 150 https://doi.org/10.1186/1475-2859-11-150 kostenfrei https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 kostenfrei http://www.microbialcellfactories.com/content/11/1/150 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 150
language	English
source	In Microbial Cell Factories 11(2012), 1, p 150 volume:11 year:2012 number:1, p 150
sourceStr	In Microbial Cell Factories 11(2012), 1, p 150 volume:11 year:2012 number:1, p 150
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm Microbiology
isfreeaccess_bool	true
container_title	Microbial Cell Factories
authorswithroles_txt_mv	Arora Pankaj @@aut@@ Sharma Ashutosh @@aut@@ Mehta Richa @@aut@@ Shenoy Belle @@aut@@ Srivastava Alok @@aut@@ Singh Vijay @@aut@@
publishDateDaySort_date	2012-01-01T00:00:00Z
hierarchy_top_id	355987651
id	DOAJ021592241
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ021592241</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230307050932.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2012 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1475-2859-11-150</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ021592241</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJd24c133271384827a683cc1b1c7c6875</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QR1-502</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Arora Pankaj</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. 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callnumber-first	Q - Science
author	Arora Pankaj
spellingShingle	Arora Pankaj misc QR1-502 misc 4-Chloro-2-nitrophenol misc 4-Chloro-2-aminophenol misc 2-aminophenol misc Biodegradation misc Bioremediation misc Soil microcosm misc Microbiology Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA
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topic_title	QR1-502 Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA 4-Chloro-2-nitrophenol 4-Chloro-2-aminophenol 2-aminophenol Biodegradation Bioremediation Soil microcosm
topic	misc QR1-502 misc 4-Chloro-2-nitrophenol misc 4-Chloro-2-aminophenol misc 2-aminophenol misc Biodegradation misc Bioremediation misc Soil microcosm misc Microbiology
topic_unstemmed	misc QR1-502 misc 4-Chloro-2-nitrophenol misc 4-Chloro-2-aminophenol misc 2-aminophenol misc Biodegradation misc Bioremediation misc Soil microcosm misc Microbiology
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title	Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA
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title_full	Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA
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author_browse	Arora Pankaj Sharma Ashutosh Mehta Richa Shenoy Belle Srivastava Alok Singh Vijay
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doi_str_mv	10.1186/1475-2859-11-150
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title_sort	metabolism of 4-chloro-2-nitrophenol in a gram-positive bacterium, <it<exiguobacterium</it< sp. pma
callnumber	QR1-502
title_auth	Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA
abstract	<p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.</p< <p<Results</p< <p<A 4C2NP-degrading bacterium, <it<Exiguobacterium</it< sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. <it<Exiguobacterium</it< sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of <it<Exiguobacterium</it< sp. PMA. The bioremediation of 4C2NP by <it<Exiguobacterium</it< sp. PMA was faster in non-sterilized soil than sterilized soil.</p< <p<Conclusions</p< <p<Our studies indicate that <it<Exiguobacterium</it< sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.</p<
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title_short	Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA
url	https://doi.org/10.1186/1475-2859-11-150 https://doaj.org/article/d24c133271384827a683cc1b1c7c6875 http://www.microbialcellfactories.com/content/11/1/150 https://doaj.org/toc/1475-2859
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Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, <it<Exiguobacterium</it< sp. PMA

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