Assessment of α-tocopherol content in cow and goat milk from the Serbian market

The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set...
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Autor*in:	Sunarić Slavica [verfasserIn] Živković Jelena [verfasserIn] Pavlović Radmila [verfasserIn] Kocić Gordana [verfasserIn] Trutić Nataša [verfasserIn] Živanović Slavoljub [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; srp

Erschienen:	2012

Schlagwörter:	α-tocopherol cow milk goat milk HPLC analysis

Übergeordnetes Werk:	In: Hemijska Industrija - Association of Chemical Engineers of Serbia, 2010, 66(2012), 4, Seite 559-566
Übergeordnetes Werk:	volume:66 ; year:2012 ; number:4 ; pages:559-566

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.2298/HEMIND111116006S

Katalog-ID:	DOAJ022076611

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650		4	\|a α-tocopherol
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allfields	10.2298/HEMIND111116006S doi (DE-627)DOAJ022076611 (DE-599)DOAJa69087703abc4e1282ef4aaeb8f9d51f DE-627 ger DE-627 rakwb eng srp TP1-1185 Sunarić Slavica verfasserin aut Assessment of α-tocopherol content in cow and goat milk from the Serbian market 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content. α-tocopherol cow milk goat milk HPLC analysis Chemical technology Živković Jelena verfasserin aut Pavlović Radmila verfasserin aut Kocić Gordana verfasserin aut Trutić Nataša verfasserin aut Živanović Slavoljub verfasserin aut In Hemijska Industrija Association of Chemical Engineers of Serbia, 2010 66(2012), 4, Seite 559-566 (DE-627)1019885629 22177426 nnns volume:66 year:2012 number:4 pages:559-566 https://doi.org/10.2298/HEMIND111116006S kostenfrei https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f kostenfrei http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf kostenfrei https://doaj.org/toc/0367-598X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 66 2012 4 559-566
spelling	10.2298/HEMIND111116006S doi (DE-627)DOAJ022076611 (DE-599)DOAJa69087703abc4e1282ef4aaeb8f9d51f DE-627 ger DE-627 rakwb eng srp TP1-1185 Sunarić Slavica verfasserin aut Assessment of α-tocopherol content in cow and goat milk from the Serbian market 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content. α-tocopherol cow milk goat milk HPLC analysis Chemical technology Živković Jelena verfasserin aut Pavlović Radmila verfasserin aut Kocić Gordana verfasserin aut Trutić Nataša verfasserin aut Živanović Slavoljub verfasserin aut In Hemijska Industrija Association of Chemical Engineers of Serbia, 2010 66(2012), 4, Seite 559-566 (DE-627)1019885629 22177426 nnns volume:66 year:2012 number:4 pages:559-566 https://doi.org/10.2298/HEMIND111116006S kostenfrei https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f kostenfrei http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf kostenfrei https://doaj.org/toc/0367-598X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 66 2012 4 559-566
allfields_unstemmed	10.2298/HEMIND111116006S doi (DE-627)DOAJ022076611 (DE-599)DOAJa69087703abc4e1282ef4aaeb8f9d51f DE-627 ger DE-627 rakwb eng srp TP1-1185 Sunarić Slavica verfasserin aut Assessment of α-tocopherol content in cow and goat milk from the Serbian market 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content. α-tocopherol cow milk goat milk HPLC analysis Chemical technology Živković Jelena verfasserin aut Pavlović Radmila verfasserin aut Kocić Gordana verfasserin aut Trutić Nataša verfasserin aut Živanović Slavoljub verfasserin aut In Hemijska Industrija Association of Chemical Engineers of Serbia, 2010 66(2012), 4, Seite 559-566 (DE-627)1019885629 22177426 nnns volume:66 year:2012 number:4 pages:559-566 https://doi.org/10.2298/HEMIND111116006S kostenfrei https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f kostenfrei http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf kostenfrei https://doaj.org/toc/0367-598X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 66 2012 4 559-566
allfieldsGer	10.2298/HEMIND111116006S doi (DE-627)DOAJ022076611 (DE-599)DOAJa69087703abc4e1282ef4aaeb8f9d51f DE-627 ger DE-627 rakwb eng srp TP1-1185 Sunarić Slavica verfasserin aut Assessment of α-tocopherol content in cow and goat milk from the Serbian market 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content. α-tocopherol cow milk goat milk HPLC analysis Chemical technology Živković Jelena verfasserin aut Pavlović Radmila verfasserin aut Kocić Gordana verfasserin aut Trutić Nataša verfasserin aut Živanović Slavoljub verfasserin aut In Hemijska Industrija Association of Chemical Engineers of Serbia, 2010 66(2012), 4, Seite 559-566 (DE-627)1019885629 22177426 nnns volume:66 year:2012 number:4 pages:559-566 https://doi.org/10.2298/HEMIND111116006S kostenfrei https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f kostenfrei http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf kostenfrei https://doaj.org/toc/0367-598X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 66 2012 4 559-566
allfieldsSound	10.2298/HEMIND111116006S doi (DE-627)DOAJ022076611 (DE-599)DOAJa69087703abc4e1282ef4aaeb8f9d51f DE-627 ger DE-627 rakwb eng srp TP1-1185 Sunarić Slavica verfasserin aut Assessment of α-tocopherol content in cow and goat milk from the Serbian market 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content. α-tocopherol cow milk goat milk HPLC analysis Chemical technology Živković Jelena verfasserin aut Pavlović Radmila verfasserin aut Kocić Gordana verfasserin aut Trutić Nataša verfasserin aut Živanović Slavoljub verfasserin aut In Hemijska Industrija Association of Chemical Engineers of Serbia, 2010 66(2012), 4, Seite 559-566 (DE-627)1019885629 22177426 nnns volume:66 year:2012 number:4 pages:559-566 https://doi.org/10.2298/HEMIND111116006S kostenfrei https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f kostenfrei http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf kostenfrei https://doaj.org/toc/0367-598X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 66 2012 4 559-566
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authorswithroles_txt_mv	Sunarić Slavica @@aut@@ Živković Jelena @@aut@@ Pavlović Radmila @@aut@@ Kocić Gordana @@aut@@ Trutić Nataša @@aut@@ Živanović Slavoljub @@aut@@
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callnumber-first	T - Technology
author	Sunarić Slavica
spellingShingle	Sunarić Slavica misc TP1-1185 misc α-tocopherol misc cow milk misc goat milk misc HPLC analysis misc Chemical technology Assessment of α-tocopherol content in cow and goat milk from the Serbian market
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topic_title	TP1-1185 Assessment of α-tocopherol content in cow and goat milk from the Serbian market α-tocopherol cow milk goat milk HPLC analysis
topic	misc TP1-1185 misc α-tocopherol misc cow milk misc goat milk misc HPLC analysis misc Chemical technology
topic_unstemmed	misc TP1-1185 misc α-tocopherol misc cow milk misc goat milk misc HPLC analysis misc Chemical technology
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title	Assessment of α-tocopherol content in cow and goat milk from the Serbian market
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title_full	Assessment of α-tocopherol content in cow and goat milk from the Serbian market
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author_browse	Sunarić Slavica Živković Jelena Pavlović Radmila Kocić Gordana Trutić Nataša Živanović Slavoljub
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title_sort	assessment of α-tocopherol content in cow and goat milk from the serbian market
callnumber	TP1-1185
title_auth	Assessment of α-tocopherol content in cow and goat milk from the Serbian market
abstract	The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content.
abstractGer	The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content.
abstract_unstemmed	The present paper reports determination of α-tocopherol in commercial cow, raw cow and goat milk, as well as estimation of its nutritive value based on α-tocopherol content. Quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 nm and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid-liquid extraction with diethyl-ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower α-tocopherol levels than milk with higher fat content. α-tocopherol concentration below 0.30 μg/ml in low-fat cow milk and 0.83-0.86 μg/ml in whole milk were detected. Raw goat milk had much more α-tocopherol (1.25 μg/ml) compared with commercial and raw cow milk with similar fat content.
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title_short	Assessment of α-tocopherol content in cow and goat milk from the Serbian market
url	https://doi.org/10.2298/HEMIND111116006S https://doaj.org/article/a69087703abc4e1282ef4aaeb8f9d51f http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200006S.pdf https://doaj.org/toc/0367-598X
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Assessment of α-tocopherol content in cow and goat milk from the Serbian market

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