Novel transgenic pigs with enhanced growth and reduced environmental impact

In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing t...
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Autor*in:	Xianwei Zhang [verfasserIn] Zicong Li [verfasserIn] Huaqiang Yang [verfasserIn] Dewu Liu [verfasserIn] Gengyuan Cai [verfasserIn] Guoling Li [verfasserIn] Jianxin Mo [verfasserIn] Dehua Wang [verfasserIn] Cuili Zhong [verfasserIn] Haoqiang Wang [verfasserIn] Yue Sun [verfasserIn] Junsong Shi [verfasserIn] Enqin Zheng [verfasserIn] Fanming Meng [verfasserIn] Mao Zhang [verfasserIn] Xiaoyan He [verfasserIn] Rong Zhou [verfasserIn] Jian Zhang [verfasserIn] Miaorong Huang [verfasserIn] Ran Zhang [verfasserIn] Ning Li [verfasserIn] Mingzhe Fan [verfasserIn] Jinzeng Yang [verfasserIn] Zhenfang Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 7(2018)
Übergeordnetes Werk:	volume:7 ; year:2018

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.34286

Katalog-ID:	DOAJ022134565

Internformat


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allfields	10.7554/eLife.34286 doi (DE-627)DOAJ022134565 (DE-599)DOAJc6d4ddbac66a4bf6821d2d76c49a419f DE-627 ger DE-627 rakwb eng QH301-705.5 Xianwei Zhang verfasserin aut Novel transgenic pigs with enhanced growth and reduced environmental impact 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides Medicine R Science Q Biology (General) Zicong Li verfasserin aut Huaqiang Yang verfasserin aut Dewu Liu verfasserin aut Gengyuan Cai verfasserin aut Guoling Li verfasserin aut Jianxin Mo verfasserin aut Dehua Wang verfasserin aut Cuili Zhong verfasserin aut Haoqiang Wang verfasserin aut Yue Sun verfasserin aut Junsong Shi verfasserin aut Enqin Zheng verfasserin aut Fanming Meng verfasserin aut Mao Zhang verfasserin aut Xiaoyan He verfasserin aut Rong Zhou verfasserin aut Jian Zhang verfasserin aut Miaorong Huang verfasserin aut Ran Zhang verfasserin aut Ning Li verfasserin aut Mingzhe Fan verfasserin aut Jinzeng Yang verfasserin aut Zhenfang Wu verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.34286 kostenfrei https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f kostenfrei https://elifesciences.org/articles/34286 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
spelling	10.7554/eLife.34286 doi (DE-627)DOAJ022134565 (DE-599)DOAJc6d4ddbac66a4bf6821d2d76c49a419f DE-627 ger DE-627 rakwb eng QH301-705.5 Xianwei Zhang verfasserin aut Novel transgenic pigs with enhanced growth and reduced environmental impact 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides Medicine R Science Q Biology (General) Zicong Li verfasserin aut Huaqiang Yang verfasserin aut Dewu Liu verfasserin aut Gengyuan Cai verfasserin aut Guoling Li verfasserin aut Jianxin Mo verfasserin aut Dehua Wang verfasserin aut Cuili Zhong verfasserin aut Haoqiang Wang verfasserin aut Yue Sun verfasserin aut Junsong Shi verfasserin aut Enqin Zheng verfasserin aut Fanming Meng verfasserin aut Mao Zhang verfasserin aut Xiaoyan He verfasserin aut Rong Zhou verfasserin aut Jian Zhang verfasserin aut Miaorong Huang verfasserin aut Ran Zhang verfasserin aut Ning Li verfasserin aut Mingzhe Fan verfasserin aut Jinzeng Yang verfasserin aut Zhenfang Wu verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.34286 kostenfrei https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f kostenfrei https://elifesciences.org/articles/34286 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfields_unstemmed	10.7554/eLife.34286 doi (DE-627)DOAJ022134565 (DE-599)DOAJc6d4ddbac66a4bf6821d2d76c49a419f DE-627 ger DE-627 rakwb eng QH301-705.5 Xianwei Zhang verfasserin aut Novel transgenic pigs with enhanced growth and reduced environmental impact 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides Medicine R Science Q Biology (General) Zicong Li verfasserin aut Huaqiang Yang verfasserin aut Dewu Liu verfasserin aut Gengyuan Cai verfasserin aut Guoling Li verfasserin aut Jianxin Mo verfasserin aut Dehua Wang verfasserin aut Cuili Zhong verfasserin aut Haoqiang Wang verfasserin aut Yue Sun verfasserin aut Junsong Shi verfasserin aut Enqin Zheng verfasserin aut Fanming Meng verfasserin aut Mao Zhang verfasserin aut Xiaoyan He verfasserin aut Rong Zhou verfasserin aut Jian Zhang verfasserin aut Miaorong Huang verfasserin aut Ran Zhang verfasserin aut Ning Li verfasserin aut Mingzhe Fan verfasserin aut Jinzeng Yang verfasserin aut Zhenfang Wu verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.34286 kostenfrei https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f kostenfrei https://elifesciences.org/articles/34286 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfieldsGer	10.7554/eLife.34286 doi (DE-627)DOAJ022134565 (DE-599)DOAJc6d4ddbac66a4bf6821d2d76c49a419f DE-627 ger DE-627 rakwb eng QH301-705.5 Xianwei Zhang verfasserin aut Novel transgenic pigs with enhanced growth and reduced environmental impact 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides Medicine R Science Q Biology (General) Zicong Li verfasserin aut Huaqiang Yang verfasserin aut Dewu Liu verfasserin aut Gengyuan Cai verfasserin aut Guoling Li verfasserin aut Jianxin Mo verfasserin aut Dehua Wang verfasserin aut Cuili Zhong verfasserin aut Haoqiang Wang verfasserin aut Yue Sun verfasserin aut Junsong Shi verfasserin aut Enqin Zheng verfasserin aut Fanming Meng verfasserin aut Mao Zhang verfasserin aut Xiaoyan He verfasserin aut Rong Zhou verfasserin aut Jian Zhang verfasserin aut Miaorong Huang verfasserin aut Ran Zhang verfasserin aut Ning Li verfasserin aut Mingzhe Fan verfasserin aut Jinzeng Yang verfasserin aut Zhenfang Wu verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.34286 kostenfrei https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f kostenfrei https://elifesciences.org/articles/34286 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfieldsSound	10.7554/eLife.34286 doi (DE-627)DOAJ022134565 (DE-599)DOAJc6d4ddbac66a4bf6821d2d76c49a419f DE-627 ger DE-627 rakwb eng QH301-705.5 Xianwei Zhang verfasserin aut Novel transgenic pigs with enhanced growth and reduced environmental impact 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides Medicine R Science Q Biology (General) Zicong Li verfasserin aut Huaqiang Yang verfasserin aut Dewu Liu verfasserin aut Gengyuan Cai verfasserin aut Guoling Li verfasserin aut Jianxin Mo verfasserin aut Dehua Wang verfasserin aut Cuili Zhong verfasserin aut Haoqiang Wang verfasserin aut Yue Sun verfasserin aut Junsong Shi verfasserin aut Enqin Zheng verfasserin aut Fanming Meng verfasserin aut Mao Zhang verfasserin aut Xiaoyan He verfasserin aut Rong Zhou verfasserin aut Jian Zhang verfasserin aut Miaorong Huang verfasserin aut Ran Zhang verfasserin aut Ning Li verfasserin aut Mingzhe Fan verfasserin aut Jinzeng Yang verfasserin aut Zhenfang Wu verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.34286 kostenfrei https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f kostenfrei https://elifesciences.org/articles/34286 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
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authorswithroles_txt_mv	Xianwei Zhang @@aut@@ Zicong Li @@aut@@ Huaqiang Yang @@aut@@ Dewu Liu @@aut@@ Gengyuan Cai @@aut@@ Guoling Li @@aut@@ Jianxin Mo @@aut@@ Dehua Wang @@aut@@ Cuili Zhong @@aut@@ Haoqiang Wang @@aut@@ Yue Sun @@aut@@ Junsong Shi @@aut@@ Enqin Zheng @@aut@@ Fanming Meng @@aut@@ Mao Zhang @@aut@@ Xiaoyan He @@aut@@ Rong Zhou @@aut@@ Jian Zhang @@aut@@ Miaorong Huang @@aut@@ Ran Zhang @@aut@@ Ning Li @@aut@@ Mingzhe Fan @@aut@@ Jinzeng Yang @@aut@@ Zhenfang Wu @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
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language_de	englisch
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callnumber-first	Q - Science
author	Xianwei Zhang
spellingShingle	Xianwei Zhang misc QH301-705.5 misc Transgenic pigs misc Salivary glands misc Nitrogen misc Phosphorus misc Nonstarch polysaccharides misc Medicine misc R misc Science misc Q misc Biology (General) Novel transgenic pigs with enhanced growth and reduced environmental impact
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topic_title	QH301-705.5 Novel transgenic pigs with enhanced growth and reduced environmental impact Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides
topic	misc QH301-705.5 misc Transgenic pigs misc Salivary glands misc Nitrogen misc Phosphorus misc Nonstarch polysaccharides misc Medicine misc R misc Science misc Q misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc Transgenic pigs misc Salivary glands misc Nitrogen misc Phosphorus misc Nonstarch polysaccharides misc Medicine misc R misc Science misc Q misc Biology (General)
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title	Novel transgenic pigs with enhanced growth and reduced environmental impact
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title_full	Novel transgenic pigs with enhanced growth and reduced environmental impact
author_sort	Xianwei Zhang
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author_browse	Xianwei Zhang Zicong Li Huaqiang Yang Dewu Liu Gengyuan Cai Guoling Li Jianxin Mo Dehua Wang Cuili Zhong Haoqiang Wang Yue Sun Junsong Shi Enqin Zheng Fanming Meng Mao Zhang Xiaoyan He Rong Zhou Jian Zhang Miaorong Huang Ran Zhang Ning Li Mingzhe Fan Jinzeng Yang Zhenfang Wu
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title_sort	novel transgenic pigs with enhanced growth and reduced environmental impact
callnumber	QH301-705.5
title_auth	Novel transgenic pigs with enhanced growth and reduced environmental impact
abstract	In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact.
abstractGer	In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact.
abstract_unstemmed	In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact.
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title_short	Novel transgenic pigs with enhanced growth and reduced environmental impact
url	https://doi.org/10.7554/eLife.34286 https://doaj.org/article/c6d4ddbac66a4bf6821d2d76c49a419f https://elifesciences.org/articles/34286 https://doaj.org/toc/2050-084X
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up_date	2024-07-04T00:20:32.134Z
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