FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-speci...
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Gespeichert in:

Autor*in:	Rainer Waadt [verfasserIn] Kenichi Hitomi [verfasserIn] Noriyuki Nishimura [verfasserIn] Chiharu Hitomi [verfasserIn] Stephen R Adams [verfasserIn] Elizabeth D Getzoff [verfasserIn] Julian I Schroeder [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	plant hormone abscisic acid FRET-based reporter

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 3(2014)
Übergeordnetes Werk:	volume:3 ; year:2014

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.01739

Katalog-ID:	DOAJ02214868X

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allfields	10.7554/eLife.01739 doi (DE-627)DOAJ02214868X (DE-599)DOAJ3f45aed78edb4b44a34ff8b9c1cbf309 DE-627 ger DE-627 rakwb eng QH301-705.5 Rainer Waadt verfasserin aut FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. plant hormone abscisic acid FRET-based reporter Medicine R Science Q Biology (General) Kenichi Hitomi verfasserin aut Noriyuki Nishimura verfasserin aut Chiharu Hitomi verfasserin aut Stephen R Adams verfasserin aut Elizabeth D Getzoff verfasserin aut Julian I Schroeder verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.01739 kostenfrei https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 kostenfrei https://elifesciences.org/articles/01739 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
spelling	10.7554/eLife.01739 doi (DE-627)DOAJ02214868X (DE-599)DOAJ3f45aed78edb4b44a34ff8b9c1cbf309 DE-627 ger DE-627 rakwb eng QH301-705.5 Rainer Waadt verfasserin aut FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. plant hormone abscisic acid FRET-based reporter Medicine R Science Q Biology (General) Kenichi Hitomi verfasserin aut Noriyuki Nishimura verfasserin aut Chiharu Hitomi verfasserin aut Stephen R Adams verfasserin aut Elizabeth D Getzoff verfasserin aut Julian I Schroeder verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.01739 kostenfrei https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 kostenfrei https://elifesciences.org/articles/01739 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
allfields_unstemmed	10.7554/eLife.01739 doi (DE-627)DOAJ02214868X (DE-599)DOAJ3f45aed78edb4b44a34ff8b9c1cbf309 DE-627 ger DE-627 rakwb eng QH301-705.5 Rainer Waadt verfasserin aut FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. plant hormone abscisic acid FRET-based reporter Medicine R Science Q Biology (General) Kenichi Hitomi verfasserin aut Noriyuki Nishimura verfasserin aut Chiharu Hitomi verfasserin aut Stephen R Adams verfasserin aut Elizabeth D Getzoff verfasserin aut Julian I Schroeder verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.01739 kostenfrei https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 kostenfrei https://elifesciences.org/articles/01739 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
allfieldsGer	10.7554/eLife.01739 doi (DE-627)DOAJ02214868X (DE-599)DOAJ3f45aed78edb4b44a34ff8b9c1cbf309 DE-627 ger DE-627 rakwb eng QH301-705.5 Rainer Waadt verfasserin aut FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. plant hormone abscisic acid FRET-based reporter Medicine R Science Q Biology (General) Kenichi Hitomi verfasserin aut Noriyuki Nishimura verfasserin aut Chiharu Hitomi verfasserin aut Stephen R Adams verfasserin aut Elizabeth D Getzoff verfasserin aut Julian I Schroeder verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.01739 kostenfrei https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 kostenfrei https://elifesciences.org/articles/01739 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
allfieldsSound	10.7554/eLife.01739 doi (DE-627)DOAJ02214868X (DE-599)DOAJ3f45aed78edb4b44a34ff8b9c1cbf309 DE-627 ger DE-627 rakwb eng QH301-705.5 Rainer Waadt verfasserin aut FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. plant hormone abscisic acid FRET-based reporter Medicine R Science Q Biology (General) Kenichi Hitomi verfasserin aut Noriyuki Nishimura verfasserin aut Chiharu Hitomi verfasserin aut Stephen R Adams verfasserin aut Elizabeth D Getzoff verfasserin aut Julian I Schroeder verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.01739 kostenfrei https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 kostenfrei https://elifesciences.org/articles/01739 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
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spellingShingle	Rainer Waadt misc QH301-705.5 misc plant hormone misc abscisic acid misc FRET-based reporter misc Medicine misc R misc Science misc Q misc Biology (General) FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
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title	FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
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title_full	FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
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title_auth	FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
abstract	Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.
abstractGer	Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.
abstract_unstemmed	Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.
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title_short	FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
url	https://doi.org/10.7554/eLife.01739 https://doaj.org/article/3f45aed78edb4b44a34ff8b9c1cbf309 https://elifesciences.org/articles/01739 https://doaj.org/toc/2050-084X
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