Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials

Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject...
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Gespeichert in:

Autor*in:	Kamila Oglęcka [verfasserIn] Padmini Rangamani [verfasserIn] Bo Liedberg [verfasserIn] Rachel S Kraut [verfasserIn] Atul N Parikh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 3(2014)
Übergeordnetes Werk:	volume:3 ; year:2014

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.03695

Katalog-ID:	DOAJ022156224

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allfields	10.7554/eLife.03695 doi (DE-627)DOAJ022156224 (DE-599)DOAJ338ff7058ff74578a322f56dc0840f8f DE-627 ger DE-627 rakwb eng QH301-705.5 Kamila Oglęcka verfasserin aut Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment. giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization Medicine R Science Q Biology (General) Padmini Rangamani verfasserin aut Bo Liedberg verfasserin aut Rachel S Kraut verfasserin aut Atul N Parikh verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.03695 kostenfrei https://doaj.org/article/338ff7058ff74578a322f56dc0840f8f kostenfrei https://elifesciences.org/articles/03695 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
spelling	10.7554/eLife.03695 doi (DE-627)DOAJ022156224 (DE-599)DOAJ338ff7058ff74578a322f56dc0840f8f DE-627 ger DE-627 rakwb eng QH301-705.5 Kamila Oglęcka verfasserin aut Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment. giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization Medicine R Science Q Biology (General) Padmini Rangamani verfasserin aut Bo Liedberg verfasserin aut Rachel S Kraut verfasserin aut Atul N Parikh verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.03695 kostenfrei https://doaj.org/article/338ff7058ff74578a322f56dc0840f8f kostenfrei https://elifesciences.org/articles/03695 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
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allfieldsGer	10.7554/eLife.03695 doi (DE-627)DOAJ022156224 (DE-599)DOAJ338ff7058ff74578a322f56dc0840f8f DE-627 ger DE-627 rakwb eng QH301-705.5 Kamila Oglęcka verfasserin aut Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment. giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization Medicine R Science Q Biology (General) Padmini Rangamani verfasserin aut Bo Liedberg verfasserin aut Rachel S Kraut verfasserin aut Atul N Parikh verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.03695 kostenfrei https://doaj.org/article/338ff7058ff74578a322f56dc0840f8f kostenfrei https://elifesciences.org/articles/03695 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
allfieldsSound	10.7554/eLife.03695 doi (DE-627)DOAJ022156224 (DE-599)DOAJ338ff7058ff74578a322f56dc0840f8f DE-627 ger DE-627 rakwb eng QH301-705.5 Kamila Oglęcka verfasserin aut Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment. giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization Medicine R Science Q Biology (General) Padmini Rangamani verfasserin aut Bo Liedberg verfasserin aut Rachel S Kraut verfasserin aut Atul N Parikh verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 3(2014) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:3 year:2014 https://doi.org/10.7554/eLife.03695 kostenfrei https://doaj.org/article/338ff7058ff74578a322f56dc0840f8f kostenfrei https://elifesciences.org/articles/03695 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014
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container_title	eLife
authorswithroles_txt_mv	Kamila Oglęcka @@aut@@ Padmini Rangamani @@aut@@ Bo Liedberg @@aut@@ Rachel S Kraut @@aut@@ Atul N Parikh @@aut@@
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callnumber-first	Q - Science
author	Kamila Oglęcka
spellingShingle	Kamila Oglęcka misc QH301-705.5 misc giant phospholipid vesicle misc lipid raft misc phase separation misc primitive osmoregulation misc compartmentalization misc Medicine misc R misc Science misc Q misc Biology (General) Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
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topic_title	QH301-705.5 Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization
topic	misc QH301-705.5 misc giant phospholipid vesicle misc lipid raft misc phase separation misc primitive osmoregulation misc compartmentalization misc Medicine misc R misc Science misc Q misc Biology (General)
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title	Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
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title_full	Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
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title_sort	oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
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title_auth	Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
abstract	Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment.
abstractGer	Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment.
abstract_unstemmed	Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment.
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title_short	Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
url	https://doi.org/10.7554/eLife.03695 https://doaj.org/article/338ff7058ff74578a322f56dc0840f8f https://elifesciences.org/articles/03695 https://doaj.org/toc/2050-084X
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Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials

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