Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat

The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequen...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Kiyotaka eNagaki [verfasserIn] Keisuke eTanaka [verfasserIn] Naoki eYamaji [verfasserIn] Hisato eKobayashi [verfasserIn] Minoru eMurata [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 6(2015)
Übergeordnetes Werk:	volume:6 ; year:2015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2015.00912

Katalog-ID:	DOAJ022792104

Internformat


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520			\|a The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.
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Indexfelder

author_variant	k e ke k e ke n e ne h e he m e me
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allfields	10.3389/fpls.2015.00912 doi (DE-627)DOAJ022792104 (DE-599)DOAJf149850594cf443eab647daa0d84ade3 DE-627 ger DE-627 rakwb eng SB1-1110 Kiyotaka eNagaki verfasserin aut Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic. Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture Keisuke eTanaka verfasserin aut Naoki eYamaji verfasserin aut Hisato eKobayashi verfasserin aut Minoru eMurata verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2015) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2015 https://doi.org/10.3389/fpls.2015.00912 kostenfrei https://doaj.org/article/f149850594cf443eab647daa0d84ade3 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
spelling	10.3389/fpls.2015.00912 doi (DE-627)DOAJ022792104 (DE-599)DOAJf149850594cf443eab647daa0d84ade3 DE-627 ger DE-627 rakwb eng SB1-1110 Kiyotaka eNagaki verfasserin aut Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic. Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture Keisuke eTanaka verfasserin aut Naoki eYamaji verfasserin aut Hisato eKobayashi verfasserin aut Minoru eMurata verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2015) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2015 https://doi.org/10.3389/fpls.2015.00912 kostenfrei https://doaj.org/article/f149850594cf443eab647daa0d84ade3 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfields_unstemmed	10.3389/fpls.2015.00912 doi (DE-627)DOAJ022792104 (DE-599)DOAJf149850594cf443eab647daa0d84ade3 DE-627 ger DE-627 rakwb eng SB1-1110 Kiyotaka eNagaki verfasserin aut Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic. Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture Keisuke eTanaka verfasserin aut Naoki eYamaji verfasserin aut Hisato eKobayashi verfasserin aut Minoru eMurata verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2015) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2015 https://doi.org/10.3389/fpls.2015.00912 kostenfrei https://doaj.org/article/f149850594cf443eab647daa0d84ade3 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsGer	10.3389/fpls.2015.00912 doi (DE-627)DOAJ022792104 (DE-599)DOAJf149850594cf443eab647daa0d84ade3 DE-627 ger DE-627 rakwb eng SB1-1110 Kiyotaka eNagaki verfasserin aut Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic. Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture Keisuke eTanaka verfasserin aut Naoki eYamaji verfasserin aut Hisato eKobayashi verfasserin aut Minoru eMurata verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2015) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2015 https://doi.org/10.3389/fpls.2015.00912 kostenfrei https://doaj.org/article/f149850594cf443eab647daa0d84ade3 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsSound	10.3389/fpls.2015.00912 doi (DE-627)DOAJ022792104 (DE-599)DOAJf149850594cf443eab647daa0d84ade3 DE-627 ger DE-627 rakwb eng SB1-1110 Kiyotaka eNagaki verfasserin aut Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic. Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture Keisuke eTanaka verfasserin aut Naoki eYamaji verfasserin aut Hisato eKobayashi verfasserin aut Minoru eMurata verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2015) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2015 https://doi.org/10.3389/fpls.2015.00912 kostenfrei https://doaj.org/article/f149850594cf443eab647daa0d84ade3 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
language	English
source	In Frontiers in Plant Science 6(2015) volume:6 year:2015
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topic_facet	Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA Plant culture
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container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Kiyotaka eNagaki @@aut@@ Keisuke eTanaka @@aut@@ Naoki eYamaji @@aut@@ Hisato eKobayashi @@aut@@ Minoru eMurata @@aut@@
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callnumber-first	S - Agriculture
author	Kiyotaka eNagaki
spellingShingle	Kiyotaka eNagaki misc SB1-1110 misc Centromere misc ChIP-seq misc Sunflower (Helianthus annuus) misc centromeric histone H3 misc centromeric DNA misc Plant culture Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat
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topic_title	SB1-1110 Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat Centromere ChIP-seq Sunflower (Helianthus annuus) centromeric histone H3 centromeric DNA
topic	misc SB1-1110 misc Centromere misc ChIP-seq misc Sunflower (Helianthus annuus) misc centromeric histone H3 misc centromeric DNA misc Plant culture
topic_unstemmed	misc SB1-1110 misc Centromere misc ChIP-seq misc Sunflower (Helianthus annuus) misc centromeric histone H3 misc centromeric DNA misc Plant culture
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title	Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat
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title_full	Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat
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title_sort	sunflower centromeres consist of a centromere-specific line and a chromosome-specific tandem repeat
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title_auth	Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat
abstract	The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.
abstractGer	The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.
abstract_unstemmed	The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.
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title_short	Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat
url	https://doi.org/10.3389/fpls.2015.00912 https://doaj.org/article/f149850594cf443eab647daa0d84ade3 http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00912/full https://doaj.org/toc/1664-462X
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up_date	2024-07-03T14:02:33.387Z
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Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat

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