Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China

Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) col...
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Autor*in:	Qianqian Wu [verfasserIn] Xin Xu [verfasserIn] Qinxi Chen [verfasserIn] Jun Ji [verfasserIn] Yunchao Kan [verfasserIn] Lunguang Yao [verfasserIn] Qingmei Xie [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites

Übergeordnetes Werk:	In: Pathogens - MDPI AG, 2012, 8(2019), 4, p 185
Übergeordnetes Werk:	volume:8 ; year:2019 ; number:4, p 185

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pathogens8040185

Katalog-ID:	DOAJ022819460

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520			\|a Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.
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allfields	10.3390/pathogens8040185 doi (DE-627)DOAJ022819460 (DE-599)DOAJfb0ca7397b16433383a1de73e59066b2 DE-627 ger DE-627 rakwb eng Qianqian Wu verfasserin aut Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern. avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites Medicine R Xin Xu verfasserin aut Qinxi Chen verfasserin aut Jun Ji verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Qingmei Xie verfasserin aut In Pathogens MDPI AG, 2012 8(2019), 4, p 185 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:8 year:2019 number:4, p 185 https://doi.org/10.3390/pathogens8040185 kostenfrei https://doaj.org/article/fb0ca7397b16433383a1de73e59066b2 kostenfrei https://www.mdpi.com/2076-0817/8/4/185 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 4, p 185
spelling	10.3390/pathogens8040185 doi (DE-627)DOAJ022819460 (DE-599)DOAJfb0ca7397b16433383a1de73e59066b2 DE-627 ger DE-627 rakwb eng Qianqian Wu verfasserin aut Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern. avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites Medicine R Xin Xu verfasserin aut Qinxi Chen verfasserin aut Jun Ji verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Qingmei Xie verfasserin aut In Pathogens MDPI AG, 2012 8(2019), 4, p 185 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:8 year:2019 number:4, p 185 https://doi.org/10.3390/pathogens8040185 kostenfrei https://doaj.org/article/fb0ca7397b16433383a1de73e59066b2 kostenfrei https://www.mdpi.com/2076-0817/8/4/185 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 4, p 185
allfields_unstemmed	10.3390/pathogens8040185 doi (DE-627)DOAJ022819460 (DE-599)DOAJfb0ca7397b16433383a1de73e59066b2 DE-627 ger DE-627 rakwb eng Qianqian Wu verfasserin aut Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern. avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites Medicine R Xin Xu verfasserin aut Qinxi Chen verfasserin aut Jun Ji verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Qingmei Xie verfasserin aut In Pathogens MDPI AG, 2012 8(2019), 4, p 185 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:8 year:2019 number:4, p 185 https://doi.org/10.3390/pathogens8040185 kostenfrei https://doaj.org/article/fb0ca7397b16433383a1de73e59066b2 kostenfrei https://www.mdpi.com/2076-0817/8/4/185 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 4, p 185
allfieldsGer	10.3390/pathogens8040185 doi (DE-627)DOAJ022819460 (DE-599)DOAJfb0ca7397b16433383a1de73e59066b2 DE-627 ger DE-627 rakwb eng Qianqian Wu verfasserin aut Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern. avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites Medicine R Xin Xu verfasserin aut Qinxi Chen verfasserin aut Jun Ji verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Qingmei Xie verfasserin aut In Pathogens MDPI AG, 2012 8(2019), 4, p 185 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:8 year:2019 number:4, p 185 https://doi.org/10.3390/pathogens8040185 kostenfrei https://doaj.org/article/fb0ca7397b16433383a1de73e59066b2 kostenfrei https://www.mdpi.com/2076-0817/8/4/185 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 4, p 185
allfieldsSound	10.3390/pathogens8040185 doi (DE-627)DOAJ022819460 (DE-599)DOAJfb0ca7397b16433383a1de73e59066b2 DE-627 ger DE-627 rakwb eng Qianqian Wu verfasserin aut Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern. avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites Medicine R Xin Xu verfasserin aut Qinxi Chen verfasserin aut Jun Ji verfasserin aut Yunchao Kan verfasserin aut Lunguang Yao verfasserin aut Qingmei Xie verfasserin aut In Pathogens MDPI AG, 2012 8(2019), 4, p 185 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:8 year:2019 number:4, p 185 https://doi.org/10.3390/pathogens8040185 kostenfrei https://doaj.org/article/fb0ca7397b16433383a1de73e59066b2 kostenfrei https://www.mdpi.com/2076-0817/8/4/185 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 4, p 185
language	English
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author	Qianqian Wu
spellingShingle	Qianqian Wu misc avian gyrovirus 2 misc variant-related gyrovirus misc sequence analysis misc phylogenetic analysis misc amino acid sites misc Medicine misc R Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China
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topic_title	Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China avian gyrovirus 2 variant-related gyrovirus sequence analysis phylogenetic analysis amino acid sites
topic	misc avian gyrovirus 2 misc variant-related gyrovirus misc sequence analysis misc phylogenetic analysis misc amino acid sites misc Medicine misc R
topic_unstemmed	misc avian gyrovirus 2 misc variant-related gyrovirus misc sequence analysis misc phylogenetic analysis misc amino acid sites misc Medicine misc R
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title	Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China
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title_full	Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China
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title_sort	genetic analysis of avian gyrovirus 2 variant-related <i<gyrovirus</i< detected in farmed king ratsnake (<i<elaphe carinata</i<): the first report from china
title_auth	Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China
abstract	Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.
abstractGer	Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.
abstract_unstemmed	Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus <i<Gyrovirus</i<. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.
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doi_str	10.3390/pathogens8040185
up_date	2024-07-03T14:11:48.076Z
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AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (<i<Elaphe carinata</i<) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. 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Genetic Analysis of Avian Gyrovirus 2 Variant-Related <i<Gyrovirus</i< Detected in Farmed King Ratsnake (<i<Elaphe carinata</i<): The First Report from China

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