Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation

Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as...
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Autor*in:	Cicia Firakania [verfasserIn] Indra G. Mansur [verfasserIn] Sri W.A. Jusman [verfasserIn] Mohamad Sadikin [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	avidin biotin cell proliferation purine de novo synthesis

Übergeordnetes Werk:	In: Medical Journal of Indonesia - Faculty of Medicine Universitas Indonesia, 2013, 25(2016), 1
Übergeordnetes Werk:	volume:25 ; year:2016 ; number:1

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.13181/mji.v25i1.1264

Katalog-ID:	DOAJ022990771

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245	1	0	\|a Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
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520			\|a Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.
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allfields	10.13181/mji.v25i1.1264 doi (DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d DE-627 ger DE-627 rakwb eng R5-920 Cicia Firakania verfasserin aut Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation. avidin biotin cell proliferation purine de novo synthesis Medicine (General) Indra G. Mansur verfasserin aut Sri W.A. Jusman verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 25(2016), 1 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:25 year:2016 number:1 https://doi.org/10.13181/mji.v25i1.1264 kostenfrei https://doaj.org/article/55cc6734c83a4e1591581c707663494d kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2016 1
spelling	10.13181/mji.v25i1.1264 doi (DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d DE-627 ger DE-627 rakwb eng R5-920 Cicia Firakania verfasserin aut Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation. avidin biotin cell proliferation purine de novo synthesis Medicine (General) Indra G. Mansur verfasserin aut Sri W.A. Jusman verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 25(2016), 1 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:25 year:2016 number:1 https://doi.org/10.13181/mji.v25i1.1264 kostenfrei https://doaj.org/article/55cc6734c83a4e1591581c707663494d kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2016 1
allfields_unstemmed	10.13181/mji.v25i1.1264 doi (DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d DE-627 ger DE-627 rakwb eng R5-920 Cicia Firakania verfasserin aut Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation. avidin biotin cell proliferation purine de novo synthesis Medicine (General) Indra G. Mansur verfasserin aut Sri W.A. Jusman verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 25(2016), 1 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:25 year:2016 number:1 https://doi.org/10.13181/mji.v25i1.1264 kostenfrei https://doaj.org/article/55cc6734c83a4e1591581c707663494d kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2016 1
allfieldsGer	10.13181/mji.v25i1.1264 doi (DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d DE-627 ger DE-627 rakwb eng R5-920 Cicia Firakania verfasserin aut Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation. avidin biotin cell proliferation purine de novo synthesis Medicine (General) Indra G. Mansur verfasserin aut Sri W.A. Jusman verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 25(2016), 1 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:25 year:2016 number:1 https://doi.org/10.13181/mji.v25i1.1264 kostenfrei https://doaj.org/article/55cc6734c83a4e1591581c707663494d kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2016 1
allfieldsSound	10.13181/mji.v25i1.1264 doi (DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d DE-627 ger DE-627 rakwb eng R5-920 Cicia Firakania verfasserin aut Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation. avidin biotin cell proliferation purine de novo synthesis Medicine (General) Indra G. Mansur verfasserin aut Sri W.A. Jusman verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 25(2016), 1 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:25 year:2016 number:1 https://doi.org/10.13181/mji.v25i1.1264 kostenfrei https://doaj.org/article/55cc6734c83a4e1591581c707663494d kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2016 1
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topic_facet	avidin biotin cell proliferation purine de novo synthesis Medicine (General)
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authorswithroles_txt_mv	Cicia Firakania @@aut@@ Indra G. Mansur @@aut@@ Sri W.A. Jusman @@aut@@ Mohamad Sadikin @@aut@@
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callnumber-first	R - Medicine
author	Cicia Firakania
spellingShingle	Cicia Firakania misc R5-920 misc avidin misc biotin misc cell proliferation misc purine de novo synthesis misc Medicine (General) Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
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topic_title	R5-920 Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation avidin biotin cell proliferation purine de novo synthesis
topic	misc R5-920 misc avidin misc biotin misc cell proliferation misc purine de novo synthesis misc Medicine (General)
topic_unstemmed	misc R5-920 misc avidin misc biotin misc cell proliferation misc purine de novo synthesis misc Medicine (General)
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title	Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
ctrlnum	(DE-627)DOAJ022990771 (DE-599)DOAJ55cc6734c83a4e1591581c707663494d
title_full	Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
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journal	Medical Journal of Indonesia
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author_browse	Cicia Firakania Indra G. Mansur Sri W.A. Jusman Mohamad Sadikin
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title_sort	avidin inhibits pha-induced human peripheral blood mononuclear cell proliferation
callnumber	R5-920
title_auth	Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
abstract	Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.
abstractGer	Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.
abstract_unstemmed	Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase. Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.
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title_short	Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
url	https://doi.org/10.13181/mji.v25i1.1264 https://doaj.org/article/55cc6734c83a4e1591581c707663494d http://mji.ui.ac.id/journal/index.php/mji/article/view/1264 https://doaj.org/toc/0853-1773 https://doaj.org/toc/2252-8083
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up_date	2024-07-03T15:08:15.643Z
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Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation

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