Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli
Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellu...
Ausführliche Beschreibung
Autor*in: |
Pradeep Kumar Jain [verfasserIn] Vivek Jain [verfasserIn] Ashish Kumar Singh [verfasserIn] Ankur Chauhan [verfasserIn] Sarika Sinha [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2013 |
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Übergeordnetes Werk: |
In: Advanced Biomedical Research - Wolters Kluwer Medknow Publications, 2013, 2(2013), 1, Seite 75-75 |
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Übergeordnetes Werk: |
volume:2 ; year:2013 ; number:1 ; pages:75-75 |
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Link aufrufen |
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DOI / URN: |
10.4103/2277-9175.115799 |
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Katalog-ID: |
DOAJ023021667 |
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245 | 1 | 0 | |a Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli |
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520 | |a Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. | ||
650 | 4 | |a Acid tolerance | |
650 | 4 | |a dehydrogenases | |
650 | 4 | |a electron transport chain | |
650 | 4 | |a external acidification | |
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653 | 0 | |a Medicine | |
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653 | 0 | |a Biology (General) | |
700 | 0 | |a Vivek Jain |e verfasserin |4 aut | |
700 | 0 | |a Ashish Kumar Singh |e verfasserin |4 aut | |
700 | 0 | |a Ankur Chauhan |e verfasserin |4 aut | |
700 | 0 | |a Sarika Sinha |e verfasserin |4 aut | |
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10.4103/2277-9175.115799 doi (DE-627)DOAJ023021667 (DE-599)DOAJc73efa54ab8e41babffce985143282eb DE-627 ger DE-627 rakwb eng QH301-705.5 Pradeep Kumar Jain verfasserin aut Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH Medicine R Biology (General) Vivek Jain verfasserin aut Ashish Kumar Singh verfasserin aut Ankur Chauhan verfasserin aut Sarika Sinha verfasserin aut In Advanced Biomedical Research Wolters Kluwer Medknow Publications, 2013 2(2013), 1, Seite 75-75 (DE-627)719751977 (DE-600)2672524-1 22779175 nnns volume:2 year:2013 number:1 pages:75-75 https://doi.org/10.4103/2277-9175.115799 kostenfrei https://doaj.org/article/c73efa54ab8e41babffce985143282eb kostenfrei http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=75;epage=75;aulast=Jain kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2013 1 75-75 |
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10.4103/2277-9175.115799 doi (DE-627)DOAJ023021667 (DE-599)DOAJc73efa54ab8e41babffce985143282eb DE-627 ger DE-627 rakwb eng QH301-705.5 Pradeep Kumar Jain verfasserin aut Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH Medicine R Biology (General) Vivek Jain verfasserin aut Ashish Kumar Singh verfasserin aut Ankur Chauhan verfasserin aut Sarika Sinha verfasserin aut In Advanced Biomedical Research Wolters Kluwer Medknow Publications, 2013 2(2013), 1, Seite 75-75 (DE-627)719751977 (DE-600)2672524-1 22779175 nnns volume:2 year:2013 number:1 pages:75-75 https://doi.org/10.4103/2277-9175.115799 kostenfrei https://doaj.org/article/c73efa54ab8e41babffce985143282eb kostenfrei http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=75;epage=75;aulast=Jain kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2013 1 75-75 |
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10.4103/2277-9175.115799 doi (DE-627)DOAJ023021667 (DE-599)DOAJc73efa54ab8e41babffce985143282eb DE-627 ger DE-627 rakwb eng QH301-705.5 Pradeep Kumar Jain verfasserin aut Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH Medicine R Biology (General) Vivek Jain verfasserin aut Ashish Kumar Singh verfasserin aut Ankur Chauhan verfasserin aut Sarika Sinha verfasserin aut In Advanced Biomedical Research Wolters Kluwer Medknow Publications, 2013 2(2013), 1, Seite 75-75 (DE-627)719751977 (DE-600)2672524-1 22779175 nnns volume:2 year:2013 number:1 pages:75-75 https://doi.org/10.4103/2277-9175.115799 kostenfrei https://doaj.org/article/c73efa54ab8e41babffce985143282eb kostenfrei http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=75;epage=75;aulast=Jain kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2013 1 75-75 |
allfieldsGer |
10.4103/2277-9175.115799 doi (DE-627)DOAJ023021667 (DE-599)DOAJc73efa54ab8e41babffce985143282eb DE-627 ger DE-627 rakwb eng QH301-705.5 Pradeep Kumar Jain verfasserin aut Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH Medicine R Biology (General) Vivek Jain verfasserin aut Ashish Kumar Singh verfasserin aut Ankur Chauhan verfasserin aut Sarika Sinha verfasserin aut In Advanced Biomedical Research Wolters Kluwer Medknow Publications, 2013 2(2013), 1, Seite 75-75 (DE-627)719751977 (DE-600)2672524-1 22779175 nnns volume:2 year:2013 number:1 pages:75-75 https://doi.org/10.4103/2277-9175.115799 kostenfrei https://doaj.org/article/c73efa54ab8e41babffce985143282eb kostenfrei http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=75;epage=75;aulast=Jain kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2013 1 75-75 |
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10.4103/2277-9175.115799 doi (DE-627)DOAJ023021667 (DE-599)DOAJc73efa54ab8e41babffce985143282eb DE-627 ger DE-627 rakwb eng QH301-705.5 Pradeep Kumar Jain verfasserin aut Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH Medicine R Biology (General) Vivek Jain verfasserin aut Ashish Kumar Singh verfasserin aut Ankur Chauhan verfasserin aut Sarika Sinha verfasserin aut In Advanced Biomedical Research Wolters Kluwer Medknow Publications, 2013 2(2013), 1, Seite 75-75 (DE-627)719751977 (DE-600)2672524-1 22779175 nnns volume:2 year:2013 number:1 pages:75-75 https://doi.org/10.4103/2277-9175.115799 kostenfrei https://doaj.org/article/c73efa54ab8e41babffce985143282eb kostenfrei http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=75;epage=75;aulast=Jain kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei https://doaj.org/toc/2277-9175 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2013 1 75-75 |
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QH301-705.5 Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli Acid tolerance dehydrogenases electron transport chain external acidification internal acidification and intracellular pH |
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evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in escherichia coli |
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Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli |
abstract |
Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. |
abstractGer |
Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. |
abstract_unstemmed |
Background: Escherichia coli have an optimum pH range of 6-7 for growth and survival that′s why, called neutrophiles. The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period. |
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Evaluation on the responses of succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase to acid shock generated acid tolerance in Escherichia coli |
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The ∆pH across the cytoplasmic membrane is linked to cellular bioenergetics and metabolism of the body which is the major supplier of the proton motive force, so homeostasis of cellular pH is essential. When challenged by low pH, protons enter the cytoplasm; as a result, mechanisms are required to alleviate the effects of lowered cytoplasmic pH. Materials and Methods: The activities of Succinate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase in acid shocked cells of E. coli DH5 α and E. coli W3110 subjected to pH 3, 4, and 5 by two types of acidification, like external (using 0.1 N HCl), external along with the monensin (1 μM) and cytoplasmic acidification using the sodium benzoate as an acid permeant (20 mM) which is coupled to the electron transport chain by the reducing power, as yet another system possessed by E. coli as an armor against harsh acidic environments. Result: Results showed that an exposure to acidic environment (pH 3, 4 and 5) for a short period of time increased the activities of these dehydrogenases in all types of acidification except cytoplasmic acidification, which shows that higher recycling of reducing power results in pumping out of protons from the cytoplasm through the electron transport chain complexes, thereby restoring the cytoplasmic pH of the bacteria in the range of 7.4-7.8. Conclusion: Study indicates that acid shocked E. coli for a period of 2 h can survive for a sustained period.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Acid tolerance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">dehydrogenases</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">electron transport chain</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">external acidification</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">internal acidification and intracellular pH</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Vivek Jain</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ashish Kumar Singh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ankur Chauhan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sarika Sinha</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Advanced Biomedical Research</subfield><subfield code="d">Wolters Kluwer Medknow Publications, 2013</subfield><subfield code="g">2(2013), 1, Seite 75-75</subfield><subfield code="w">(DE-627)719751977</subfield><subfield code="w">(DE-600)2672524-1</subfield><subfield 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