Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study

Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Setu Mathur [verfasserIn] Shaila Veerappa Kothiwale [verfasserIn] Buddhi Prakash Nag [verfasserIn] Tanu Mathur [verfasserIn] Ashoka Bhansali [verfasserIn] Rohit Kumar Khatri [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia

Übergeordnetes Werk:	In: Journal of Indian Society of Periodontology - Wolters Kluwer Medknow Publications, 2009, 20(2016), 4, Seite 369-373
Übergeordnetes Werk:	volume:20 ; year:2016 ; number:4 ; pages:369-373

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4103/0972-124X.186935

Katalog-ID:	DOAJ023167092

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520			\|a Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future.
650		4	\|a Fusobacterium nucleatum
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allfields	10.4103/0972-124X.186935 doi (DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9 DE-627 ger DE-627 rakwb eng RK1-715 Setu Mathur verfasserin aut Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry Shaila Veerappa Kothiwale verfasserin aut Buddhi Prakash Nag verfasserin aut Tanu Mathur verfasserin aut Ashoka Bhansali verfasserin aut Rohit Kumar Khatri verfasserin aut In Journal of Indian Society of Periodontology Wolters Kluwer Medknow Publications, 2009 20(2016), 4, Seite 369-373 (DE-627)622513702 (DE-600)2545458-4 09751580 nnns volume:20 year:2016 number:4 pages:369-373 https://doi.org/10.4103/0972-124X.186935 kostenfrei https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 kostenfrei http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur kostenfrei https://doaj.org/toc/0972-124X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2016 4 369-373
spelling	10.4103/0972-124X.186935 doi (DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9 DE-627 ger DE-627 rakwb eng RK1-715 Setu Mathur verfasserin aut Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry Shaila Veerappa Kothiwale verfasserin aut Buddhi Prakash Nag verfasserin aut Tanu Mathur verfasserin aut Ashoka Bhansali verfasserin aut Rohit Kumar Khatri verfasserin aut In Journal of Indian Society of Periodontology Wolters Kluwer Medknow Publications, 2009 20(2016), 4, Seite 369-373 (DE-627)622513702 (DE-600)2545458-4 09751580 nnns volume:20 year:2016 number:4 pages:369-373 https://doi.org/10.4103/0972-124X.186935 kostenfrei https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 kostenfrei http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur kostenfrei https://doaj.org/toc/0972-124X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2016 4 369-373
allfields_unstemmed	10.4103/0972-124X.186935 doi (DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9 DE-627 ger DE-627 rakwb eng RK1-715 Setu Mathur verfasserin aut Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry Shaila Veerappa Kothiwale verfasserin aut Buddhi Prakash Nag verfasserin aut Tanu Mathur verfasserin aut Ashoka Bhansali verfasserin aut Rohit Kumar Khatri verfasserin aut In Journal of Indian Society of Periodontology Wolters Kluwer Medknow Publications, 2009 20(2016), 4, Seite 369-373 (DE-627)622513702 (DE-600)2545458-4 09751580 nnns volume:20 year:2016 number:4 pages:369-373 https://doi.org/10.4103/0972-124X.186935 kostenfrei https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 kostenfrei http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur kostenfrei https://doaj.org/toc/0972-124X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2016 4 369-373
allfieldsGer	10.4103/0972-124X.186935 doi (DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9 DE-627 ger DE-627 rakwb eng RK1-715 Setu Mathur verfasserin aut Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry Shaila Veerappa Kothiwale verfasserin aut Buddhi Prakash Nag verfasserin aut Tanu Mathur verfasserin aut Ashoka Bhansali verfasserin aut Rohit Kumar Khatri verfasserin aut In Journal of Indian Society of Periodontology Wolters Kluwer Medknow Publications, 2009 20(2016), 4, Seite 369-373 (DE-627)622513702 (DE-600)2545458-4 09751580 nnns volume:20 year:2016 number:4 pages:369-373 https://doi.org/10.4103/0972-124X.186935 kostenfrei https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 kostenfrei http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur kostenfrei https://doaj.org/toc/0972-124X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2016 4 369-373
allfieldsSound	10.4103/0972-124X.186935 doi (DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9 DE-627 ger DE-627 rakwb eng RK1-715 Setu Mathur verfasserin aut Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future. Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry Shaila Veerappa Kothiwale verfasserin aut Buddhi Prakash Nag verfasserin aut Tanu Mathur verfasserin aut Ashoka Bhansali verfasserin aut Rohit Kumar Khatri verfasserin aut In Journal of Indian Society of Periodontology Wolters Kluwer Medknow Publications, 2009 20(2016), 4, Seite 369-373 (DE-627)622513702 (DE-600)2545458-4 09751580 nnns volume:20 year:2016 number:4 pages:369-373 https://doi.org/10.4103/0972-124X.186935 kostenfrei https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 kostenfrei http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur kostenfrei https://doaj.org/toc/0972-124X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2016 4 369-373
language	English
source	In Journal of Indian Society of Periodontology 20(2016), 4, Seite 369-373 volume:20 year:2016 number:4 pages:369-373
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topic_facet	Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia Dentistry
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authorswithroles_txt_mv	Setu Mathur @@aut@@ Shaila Veerappa Kothiwale @@aut@@ Buddhi Prakash Nag @@aut@@ Tanu Mathur @@aut@@ Ashoka Bhansali @@aut@@ Rohit Kumar Khatri @@aut@@
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author	Setu Mathur
spellingShingle	Setu Mathur misc RK1-715 misc Fusobacterium nucleatum misc periodontitis misc photodynamic therapy misc Porphyromonas gingivalis misc Prevotella intermedia misc Dentistry Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study
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issn	09751580
topic_title	RK1-715 Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study Fusobacterium nucleatum periodontitis photodynamic therapy Porphyromonas gingivalis Prevotella intermedia
topic	misc RK1-715 misc Fusobacterium nucleatum misc periodontitis misc photodynamic therapy misc Porphyromonas gingivalis misc Prevotella intermedia misc Dentistry
topic_unstemmed	misc RK1-715 misc Fusobacterium nucleatum misc periodontitis misc photodynamic therapy misc Porphyromonas gingivalis misc Prevotella intermedia misc Dentistry
topic_browse	misc RK1-715 misc Fusobacterium nucleatum misc periodontitis misc photodynamic therapy misc Porphyromonas gingivalis misc Prevotella intermedia misc Dentistry
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title	Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study
ctrlnum	(DE-627)DOAJ023167092 (DE-599)DOAJd3eb7dc2d0cd4bc9b980a5a6ccac4fd9
title_full	Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study
author_sort	Setu Mathur
journal	Journal of Indian Society of Periodontology
journalStr	Journal of Indian Society of Periodontology
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author_browse	Setu Mathur Shaila Veerappa Kothiwale Buddhi Prakash Nag Tanu Mathur Ashoka Bhansali Rohit Kumar Khatri
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author-letter	Setu Mathur
doi_str_mv	10.4103/0972-124X.186935
author2-role	verfasserin
title_sort	effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: an in vitro study
callnumber	RK1-715
title_auth	Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study
abstract	Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future.
abstractGer	Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future.
abstract_unstemmed	Background: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser. Aim: This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Settings: The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum. Design: In vitro study design. Materials and Methods: Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed. Statistical Analysis: Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann–Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group. Results: The results revealed substantial reduction in the viable bacterial count. F. nucleatum was found to be most sensitive to killing by laser irradiation followed by P. intermedia and then P. gingivalis. Further, the TBO dye per se did not have any significant bactericidal effect. Conclusion: Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future.
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container_issue	4
title_short	Effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: An in vitro study
url	https://doi.org/10.4103/0972-124X.186935 https://doaj.org/article/d3eb7dc2d0cd4bc9b980a5a6ccac4fd9 http://www.jisponline.com/article.asp?issn=0972-124X;year=2016;volume=20;issue=4;spage=369;epage=373;aulast=Mathur https://doaj.org/toc/0972-124X
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author2	Shaila Veerappa Kothiwale Buddhi Prakash Nag Tanu Mathur Ashoka Bhansali Rohit Kumar Khatri
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up_date	2024-07-03T16:06:32.920Z
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