Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection

The lack of authentic standards limits the quantitative analysis of herbal drugs in biological samples. This present work demonstrated a practicable assay of herbs and their metabolites independent of the availability of authentic standards. A liquid chromatography–electrospray ionization–mass spect...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Youping Liu [verfasserIn] Xin Di [verfasserIn] Yin Wang [verfasserIn] Qier Mu [verfasserIn] Rui Yan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	metabolite determination liquid chromatography tandem mass spectrometry Evodiae fructus Zuojinwan preparation

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 16(2011), 7, Seite 5822-5832
Übergeordnetes Werk:	volume:16 ; year:2011 ; number:7 ; pages:5822-5832

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules16075822

Katalog-ID:	DOAJ023167580

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language	English
source	In Molecules 16(2011), 7, Seite 5822-5832 volume:16 year:2011 number:7 pages:5822-5832
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topic_facet	metabolite determination liquid chromatography tandem mass spectrometry Evodiae fructus Zuojinwan preparation Organic chemistry
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container_title	Molecules
authorswithroles_txt_mv	Youping Liu @@aut@@ Xin Di @@aut@@ Yin Wang @@aut@@ Qier Mu @@aut@@ Rui Yan @@aut@@
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callnumber-first	Q - Science
author	Youping Liu
spellingShingle	Youping Liu misc QD241-441 misc metabolite misc determination misc liquid chromatography tandem mass spectrometry misc Evodiae fructus misc Zuojinwan preparation misc Organic chemistry Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection
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topic_title	QD241-441 Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection metabolite determination liquid chromatography tandem mass spectrometry Evodiae fructus Zuojinwan preparation
topic	misc QD241-441 misc metabolite misc determination misc liquid chromatography tandem mass spectrometry misc Evodiae fructus misc Zuojinwan preparation misc Organic chemistry
topic_unstemmed	misc QD241-441 misc metabolite misc determination misc liquid chromatography tandem mass spectrometry misc Evodiae fructus misc Zuojinwan preparation misc Organic chemistry
topic_browse	misc QD241-441 misc metabolite misc determination misc liquid chromatography tandem mass spectrometry misc Evodiae fructus misc Zuojinwan preparation misc Organic chemistry
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title	Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection
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title_full	Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection
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title_sort	characterization and determination of the alkaloid metabolites of evodiae fructus in rat urine by liquid chromatography-tandem mass spectrometry detection
callnumber	QD241-441
title_auth	Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection
abstract	The lack of authentic standards limits the quantitative analysis of herbal drugs in biological samples. This present work demonstrated a practicable assay of herbs and their metabolites independent of the availability of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites after oral administration of Evodiae fructus and Zuojinwan preparation in rat urine has been developed. Urine samples extracted with a protein precipitation procedure were separated on a C18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. The detection was performed by MS with electrospray ionization interface in positive selected reaction monitoring (SRM) mode. One urine sample after administration was selected as 'standard'. The method validation was carried out according to a conventional method that was calibrated by authentic standards. The fully validated method was applied to the pharmacokinetic study of the metabolites in rat urine. The results could provide evidence to explain the combination of Coptidis rhizoma and Evodiae fructus in terms of elimination.
abstractGer	The lack of authentic standards limits the quantitative analysis of herbal drugs in biological samples. This present work demonstrated a practicable assay of herbs and their metabolites independent of the availability of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites after oral administration of Evodiae fructus and Zuojinwan preparation in rat urine has been developed. Urine samples extracted with a protein precipitation procedure were separated on a C18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. The detection was performed by MS with electrospray ionization interface in positive selected reaction monitoring (SRM) mode. One urine sample after administration was selected as 'standard'. The method validation was carried out according to a conventional method that was calibrated by authentic standards. The fully validated method was applied to the pharmacokinetic study of the metabolites in rat urine. The results could provide evidence to explain the combination of Coptidis rhizoma and Evodiae fructus in terms of elimination.
abstract_unstemmed	The lack of authentic standards limits the quantitative analysis of herbal drugs in biological samples. This present work demonstrated a practicable assay of herbs and their metabolites independent of the availability of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites after oral administration of Evodiae fructus and Zuojinwan preparation in rat urine has been developed. Urine samples extracted with a protein precipitation procedure were separated on a C18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. The detection was performed by MS with electrospray ionization interface in positive selected reaction monitoring (SRM) mode. One urine sample after administration was selected as 'standard'. The method validation was carried out according to a conventional method that was calibrated by authentic standards. The fully validated method was applied to the pharmacokinetic study of the metabolites in rat urine. The results could provide evidence to explain the combination of Coptidis rhizoma and Evodiae fructus in terms of elimination.
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title_short	Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection
url	https://doi.org/10.3390/molecules16075822 https://doaj.org/article/644f938ddf6c4d248eb11f548ae15ff7 http://www.mdpi.com/1420-3049/16/7/5822/ https://doaj.org/toc/1420-3049
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Characterization and Determination of the Alkaloid Metabolites of Evodiae fructus in Rat Urine by Liquid Chromatography-Tandem Mass Spectrometry Detection

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