Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite

The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-un...
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Autor*in:	Christian Deininger [verfasserIn] Andrea Wagner [verfasserIn] Patrick Heimel [verfasserIn] Elias Salzer [verfasserIn] Xavier Monforte Vila [verfasserIn] Nadja Weißenbacher [verfasserIn] Johannes Grillari [verfasserIn] Heinz Redl [verfasserIn] Florian Wichlas [verfasserIn] Thomas Freude [verfasserIn] Herbert Tempfer [verfasserIn] Andreas Herbert Teuschl-Woller [verfasserIn] Andreas Traweger [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	silk scaffold pseudarthrosis nonunion critical sized defect bone regeneration

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2021), 1, p 283
Übergeordnetes Werk:	volume:23 ; year:2021 ; number:1, p 283

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23010283

Katalog-ID:	DOAJ023379189

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allfieldsSound	10.3390/ijms23010283 doi (DE-627)DOAJ023379189 (DE-599)DOAJ7466703d3cd84848b191e169ca3516d5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Christian Deininger verfasserin aut Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing. silk scaffold pseudarthrosis nonunion critical sized defect bone regeneration Biology (General) Chemistry Andrea Wagner verfasserin aut Patrick Heimel verfasserin aut Elias Salzer verfasserin aut Xavier Monforte Vila verfasserin aut Nadja Weißenbacher verfasserin aut Johannes Grillari verfasserin aut Heinz Redl verfasserin aut Florian Wichlas verfasserin aut Thomas Freude verfasserin aut Herbert Tempfer verfasserin aut Andreas Herbert Teuschl-Woller verfasserin aut Andreas Traweger verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2021), 1, p 283 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2021 number:1, p 283 https://doi.org/10.3390/ijms23010283 kostenfrei https://doaj.org/article/7466703d3cd84848b191e169ca3516d5 kostenfrei https://www.mdpi.com/1422-0067/23/1/283 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2021 1, p 283
language	English
source	In International Journal of Molecular Sciences 23(2021), 1, p 283 volume:23 year:2021 number:1, p 283
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callnumber-first	Q - Science
author	Christian Deininger
spellingShingle	Christian Deininger misc QH301-705.5 misc QD1-999 misc silk scaffold misc pseudarthrosis misc nonunion misc critical sized defect misc bone regeneration misc Biology (General) misc Chemistry Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite
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topic_title	QH301-705.5 QD1-999 Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite silk scaffold pseudarthrosis nonunion critical sized defect bone regeneration
topic	misc QH301-705.5 misc QD1-999 misc silk scaffold misc pseudarthrosis misc nonunion misc critical sized defect misc bone regeneration misc Biology (General) misc Chemistry
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title_auth	Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite
abstract	The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.
abstractGer	The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.
abstract_unstemmed	The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.
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container_issue	1, p 283
title_short	Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite
url	https://doi.org/10.3390/ijms23010283 https://doaj.org/article/7466703d3cd84848b191e169ca3516d5 https://www.mdpi.com/1422-0067/23/1/283 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Andrea Wagner Patrick Heimel Elias Salzer Xavier Monforte Vila Nadja Weißenbacher Johannes Grillari Heinz Redl Florian Wichlas Thomas Freude Herbert Tempfer Andreas Herbert Teuschl-Woller Andreas Traweger
author2Str	Andrea Wagner Patrick Heimel Elias Salzer Xavier Monforte Vila Nadja Weißenbacher Johannes Grillari Heinz Redl Florian Wichlas Thomas Freude Herbert Tempfer Andreas Herbert Teuschl-Woller Andreas Traweger
ppnlink	316340715
callnumber-subject	QH - Natural History and Biology
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doi_str	10.3390/ijms23010283
callnumber-a	QH301-705.5
up_date	2024-07-03T17:16:30.268Z
_version_	1803579021348306945
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