Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells

Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its t...
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Gespeichert in:

Autor*in:	Mauricio Serrano-Rubi [verfasserIn] Lidia Jimenez [verfasserIn] Jacqueline Martinez-Rendon [verfasserIn] Marcelino Cereijido [verfasserIn] Arturo Ponce [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	gap junctions ouabain cancer

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2020), 1, p 358
Übergeordnetes Werk:	volume:22 ; year:2020 ; number:1, p 358

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms22010358

Katalog-ID:	DOAJ023451793

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allfields	10.3390/ijms22010358 doi (DE-627)DOAJ023451793 (DE-599)DOAJe36ba374123446fe945870dcb69d5e6a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mauricio Serrano-Rubi verfasserin aut Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer. gap junctions ouabain cancer Biology (General) Chemistry Lidia Jimenez verfasserin aut Jacqueline Martinez-Rendon verfasserin aut Marcelino Cereijido verfasserin aut Arturo Ponce verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2020), 1, p 358 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2020 number:1, p 358 https://doi.org/10.3390/ijms22010358 kostenfrei https://doaj.org/article/e36ba374123446fe945870dcb69d5e6a kostenfrei https://www.mdpi.com/1422-0067/22/1/358 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1, p 358
spelling	10.3390/ijms22010358 doi (DE-627)DOAJ023451793 (DE-599)DOAJe36ba374123446fe945870dcb69d5e6a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mauricio Serrano-Rubi verfasserin aut Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer. gap junctions ouabain cancer Biology (General) Chemistry Lidia Jimenez verfasserin aut Jacqueline Martinez-Rendon verfasserin aut Marcelino Cereijido verfasserin aut Arturo Ponce verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2020), 1, p 358 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2020 number:1, p 358 https://doi.org/10.3390/ijms22010358 kostenfrei https://doaj.org/article/e36ba374123446fe945870dcb69d5e6a kostenfrei https://www.mdpi.com/1422-0067/22/1/358 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1, p 358
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allfieldsGer	10.3390/ijms22010358 doi (DE-627)DOAJ023451793 (DE-599)DOAJe36ba374123446fe945870dcb69d5e6a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mauricio Serrano-Rubi verfasserin aut Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer. gap junctions ouabain cancer Biology (General) Chemistry Lidia Jimenez verfasserin aut Jacqueline Martinez-Rendon verfasserin aut Marcelino Cereijido verfasserin aut Arturo Ponce verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2020), 1, p 358 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2020 number:1, p 358 https://doi.org/10.3390/ijms22010358 kostenfrei https://doaj.org/article/e36ba374123446fe945870dcb69d5e6a kostenfrei https://www.mdpi.com/1422-0067/22/1/358 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1, p 358
allfieldsSound	10.3390/ijms22010358 doi (DE-627)DOAJ023451793 (DE-599)DOAJe36ba374123446fe945870dcb69d5e6a DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mauricio Serrano-Rubi verfasserin aut Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer. gap junctions ouabain cancer Biology (General) Chemistry Lidia Jimenez verfasserin aut Jacqueline Martinez-Rendon verfasserin aut Marcelino Cereijido verfasserin aut Arturo Ponce verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2020), 1, p 358 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2020 number:1, p 358 https://doi.org/10.3390/ijms22010358 kostenfrei https://doaj.org/article/e36ba374123446fe945870dcb69d5e6a kostenfrei https://www.mdpi.com/1422-0067/22/1/358 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1, p 358
language	English
source	In International Journal of Molecular Sciences 22(2020), 1, p 358 volume:22 year:2020 number:1, p 358
sourceStr	In International Journal of Molecular Sciences 22(2020), 1, p 358 volume:22 year:2020 number:1, p 358
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authorswithroles_txt_mv	Mauricio Serrano-Rubi @@aut@@ Lidia Jimenez @@aut@@ Jacqueline Martinez-Rendon @@aut@@ Marcelino Cereijido @@aut@@ Arturo Ponce @@aut@@
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callnumber-first	Q - Science
author	Mauricio Serrano-Rubi
spellingShingle	Mauricio Serrano-Rubi misc QH301-705.5 misc QD1-999 misc gap junctions misc ouabain misc cancer misc Biology (General) misc Chemistry Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells
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topic	misc QH301-705.5 misc QD1-999 misc gap junctions misc ouabain misc cancer misc Biology (General) misc Chemistry
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title	Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells
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title_sort	ouabain promotes gap junctional intercellular communication in cancer cells
callnumber	QH301-705.5
title_auth	Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells
abstract	Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer.
abstractGer	Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer.
abstract_unstemmed	Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer.
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title_short	Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells
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up_date	2024-07-03T17:41:03.827Z
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