Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers

Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was i...
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Autor*in:	Göksel ÖZER [verfasserIn] Muhammad SAMEEULLAH [verfasserIn] Harun BAYRAKTAR [verfasserIn] Mehmet GÖRE [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	iPBS

Übergeordnetes Werk:	In: Phytopathologia Mediterranea - Firenze University Press, 2017, 56(2017), 2
Übergeordnetes Werk:	volume:56 ; year:2017 ; number:2

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.14601/Phytopathol_Mediterr-20379

Katalog-ID:	DOAJ023724145

Internformat


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912			\|a GBV_ILN_4346
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allfields	10.14601/Phytopathol_Mediterr-20379 doi (DE-627)DOAJ023724145 (DE-599)DOAJ60c84eac91d84e7b85fc1ca0fa8585fa DE-627 ger DE-627 rakwb eng QK1-989 Göksel ÖZER verfasserin aut Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea. iPBS Botany Muhammad SAMEEULLAH verfasserin aut Harun BAYRAKTAR verfasserin aut Mehmet GÖRE verfasserin aut In Phytopathologia Mediterranea Firenze University Press, 2017 56(2017), 2 (DE-627)377274410 (DE-600)2132126-7 15932095 nnns volume:56 year:2017 number:2 https://doi.org/10.14601/Phytopathol_Mediterr-20379 kostenfrei https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa kostenfrei https://oajournals.fupress.net/index.php/pm/article/view/5727 kostenfrei https://doaj.org/toc/0031-9465 Journal toc kostenfrei https://doaj.org/toc/1593-2095 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2044 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2107 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 56 2017 2
spelling	10.14601/Phytopathol_Mediterr-20379 doi (DE-627)DOAJ023724145 (DE-599)DOAJ60c84eac91d84e7b85fc1ca0fa8585fa DE-627 ger DE-627 rakwb eng QK1-989 Göksel ÖZER verfasserin aut Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea. iPBS Botany Muhammad SAMEEULLAH verfasserin aut Harun BAYRAKTAR verfasserin aut Mehmet GÖRE verfasserin aut In Phytopathologia Mediterranea Firenze University Press, 2017 56(2017), 2 (DE-627)377274410 (DE-600)2132126-7 15932095 nnns volume:56 year:2017 number:2 https://doi.org/10.14601/Phytopathol_Mediterr-20379 kostenfrei https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa kostenfrei https://oajournals.fupress.net/index.php/pm/article/view/5727 kostenfrei https://doaj.org/toc/0031-9465 Journal toc kostenfrei https://doaj.org/toc/1593-2095 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2044 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2107 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 56 2017 2
allfields_unstemmed	10.14601/Phytopathol_Mediterr-20379 doi (DE-627)DOAJ023724145 (DE-599)DOAJ60c84eac91d84e7b85fc1ca0fa8585fa DE-627 ger DE-627 rakwb eng QK1-989 Göksel ÖZER verfasserin aut Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea. iPBS Botany Muhammad SAMEEULLAH verfasserin aut Harun BAYRAKTAR verfasserin aut Mehmet GÖRE verfasserin aut In Phytopathologia Mediterranea Firenze University Press, 2017 56(2017), 2 (DE-627)377274410 (DE-600)2132126-7 15932095 nnns volume:56 year:2017 number:2 https://doi.org/10.14601/Phytopathol_Mediterr-20379 kostenfrei https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa kostenfrei https://oajournals.fupress.net/index.php/pm/article/view/5727 kostenfrei https://doaj.org/toc/0031-9465 Journal toc kostenfrei https://doaj.org/toc/1593-2095 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2044 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2107 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 56 2017 2
allfieldsGer	10.14601/Phytopathol_Mediterr-20379 doi (DE-627)DOAJ023724145 (DE-599)DOAJ60c84eac91d84e7b85fc1ca0fa8585fa DE-627 ger DE-627 rakwb eng QK1-989 Göksel ÖZER verfasserin aut Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea. iPBS Botany Muhammad SAMEEULLAH verfasserin aut Harun BAYRAKTAR verfasserin aut Mehmet GÖRE verfasserin aut In Phytopathologia Mediterranea Firenze University Press, 2017 56(2017), 2 (DE-627)377274410 (DE-600)2132126-7 15932095 nnns volume:56 year:2017 number:2 https://doi.org/10.14601/Phytopathol_Mediterr-20379 kostenfrei https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa kostenfrei https://oajournals.fupress.net/index.php/pm/article/view/5727 kostenfrei https://doaj.org/toc/0031-9465 Journal toc kostenfrei https://doaj.org/toc/1593-2095 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2044 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2107 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 56 2017 2
allfieldsSound	10.14601/Phytopathol_Mediterr-20379 doi (DE-627)DOAJ023724145 (DE-599)DOAJ60c84eac91d84e7b85fc1ca0fa8585fa DE-627 ger DE-627 rakwb eng QK1-989 Göksel ÖZER verfasserin aut Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea. iPBS Botany Muhammad SAMEEULLAH verfasserin aut Harun BAYRAKTAR verfasserin aut Mehmet GÖRE verfasserin aut In Phytopathologia Mediterranea Firenze University Press, 2017 56(2017), 2 (DE-627)377274410 (DE-600)2132126-7 15932095 nnns volume:56 year:2017 number:2 https://doi.org/10.14601/Phytopathol_Mediterr-20379 kostenfrei https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa kostenfrei https://oajournals.fupress.net/index.php/pm/article/view/5727 kostenfrei https://doaj.org/toc/0031-9465 Journal toc kostenfrei https://doaj.org/toc/1593-2095 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2044 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2107 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 56 2017 2
language	English
source	In Phytopathologia Mediterranea 56(2017), 2 volume:56 year:2017 number:2
sourceStr	In Phytopathologia Mediterranea 56(2017), 2 volume:56 year:2017 number:2
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	iPBS Botany
isfreeaccess_bool	true
container_title	Phytopathologia Mediterranea
authorswithroles_txt_mv	Göksel ÖZER @@aut@@ Muhammad SAMEEULLAH @@aut@@ Harun BAYRAKTAR @@aut@@ Mehmet GÖRE @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	377274410
id	DOAJ023724145
language_de	englisch
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callnumber-first	Q - Science
author	Göksel ÖZER
spellingShingle	Göksel ÖZER misc QK1-989 misc iPBS misc Botany Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers
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topic_title	QK1-989 Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers iPBS
topic	misc QK1-989 misc iPBS misc Botany
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title	Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers
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title_full	Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers
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title_sort	genetic diversity among phytopathogenic sclerotiniaceae, based on retrotransposon molecular markers
callnumber	QK1-989
title_auth	Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers
abstract	Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea.
abstractGer	Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea.
abstract_unstemmed	Molecular marker systems have been widely used for determination, discrimination and population structure analysis in the Sclerotiniaceae. The usefulness of a new marker system iPBS, based on the sequences of reverse transcriptase primer binding sites in long terminal repeats retrotransposons, was investigated with 34 isolates of six species confirmed by species-specific markers. Six of the iPBS primers were found to produce highly polymorphic (98%) and very distinct species-specific band patterns. Each primer amplified, on average, 33.5 polymorphic bands that was sufﬁcient for species differentiation. The polymorphism information content was 0.896, indicating better discriminating power of markers, , the Shannon’s information index was 0.438, and the genetic distance was 0.707 as average values. UPGMA cluster analysis based on retrotransposons divided all the isolates into three cluster and six sub-clusters in accordance with their species. Principal co-ordinate analysis also strongly confirmed this cluster pattern. The iPBS marker system was therefore a useful tool for evaluation of genetic variation at intra- and inter-species, and at the population levels for members of the Sclerotiniaceae. Furthermore, the iPBS markers could provide easy discrimination of Botrytis cinerea from Botrytis pseudocinerea.
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container_issue	2
title_short	Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers
url	https://doi.org/10.14601/Phytopathol_Mediterr-20379 https://doaj.org/article/60c84eac91d84e7b85fc1ca0fa8585fa https://oajournals.fupress.net/index.php/pm/article/view/5727 https://doaj.org/toc/0031-9465 https://doaj.org/toc/1593-2095
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up_date	2024-07-03T19:09:48.061Z
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Genetic diversity among phytopathogenic Sclerotiniaceae, based on retrotransposon molecular markers

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