Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Sn...
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Autor*in:	Cory Juntunen [verfasserIn] Isabel M. Woller [verfasserIn] Yongjin Sung [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	hyperspectral imaging fluorescence microscopy snapshot tomography

Übergeordnetes Werk:	In: Sensors - MDPI AG, 2003, 21(2021), 11, p 3652
Übergeordnetes Werk:	volume:21 ; year:2021 ; number:11, p 3652

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/s21113652

Katalog-ID:	DOAJ023944250

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source	In Sensors 21(2021), 11, p 3652 volume:21 year:2021 number:11, p 3652
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callnumber-first	T - Technology
author	Cory Juntunen
spellingShingle	Cory Juntunen misc TP1-1185 misc hyperspectral imaging misc fluorescence microscopy misc snapshot tomography misc Chemical technology Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography
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topic_title	TP1-1185 Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography hyperspectral imaging fluorescence microscopy snapshot tomography
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title_auth	Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography
abstract	Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.
abstractGer	Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.
abstract_unstemmed	Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.
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title_short	Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography
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Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

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