Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells

Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated...
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Autor*in:	Choo-Ryung Chung [verfasserIn] Young-Ju Oh [verfasserIn] Ha-Na Kim [verfasserIn] Yeul Park [verfasserIn] Min-Jeong Kim [verfasserIn] Su-Jung Shin [verfasserIn] Yoon-Jeong Choi [verfasserIn] Kyung-Ho Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Übergeordnetes Werk:	In: Restorative Dentistry & Endodontics - Korean Academy of Conservative Dentistry, 2017, 37(2012), 1, Seite 34-40
Übergeordnetes Werk:	volume:37 ; year:2012 ; number:1 ; pages:34-40

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5395/rde.2012.37.1.34

Katalog-ID:	DOAJ023978228

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520			\|a Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
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allfields	10.5395/rde.2012.37.1.34 doi (DE-627)DOAJ023978228 (DE-599)DOAJ716e085e92714d5aa29b4fc6f5d070e2 DE-627 ger DE-627 rakwb eng RK1-715 Choo-Ryung Chung verfasserin aut Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation. Dentistry Young-Ju Oh verfasserin aut Ha-Na Kim verfasserin aut Yeul Park verfasserin aut Min-Jeong Kim verfasserin aut Su-Jung Shin verfasserin aut Yoon-Jeong Choi verfasserin aut Kyung-Ho Kim verfasserin aut In Restorative Dentistry & Endodontics Korean Academy of Conservative Dentistry, 2017 37(2012), 1, Seite 34-40 (DE-627)746065167 (DE-600)2715357-5 22347666 nnns volume:37 year:2012 number:1 pages:34-40 https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 kostenfrei https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/toc/2234-7658 Journal toc kostenfrei https://doaj.org/toc/2234-7666 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 37 2012 1 34-40
spelling	10.5395/rde.2012.37.1.34 doi (DE-627)DOAJ023978228 (DE-599)DOAJ716e085e92714d5aa29b4fc6f5d070e2 DE-627 ger DE-627 rakwb eng RK1-715 Choo-Ryung Chung verfasserin aut Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation. Dentistry Young-Ju Oh verfasserin aut Ha-Na Kim verfasserin aut Yeul Park verfasserin aut Min-Jeong Kim verfasserin aut Su-Jung Shin verfasserin aut Yoon-Jeong Choi verfasserin aut Kyung-Ho Kim verfasserin aut In Restorative Dentistry & Endodontics Korean Academy of Conservative Dentistry, 2017 37(2012), 1, Seite 34-40 (DE-627)746065167 (DE-600)2715357-5 22347666 nnns volume:37 year:2012 number:1 pages:34-40 https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 kostenfrei https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/toc/2234-7658 Journal toc kostenfrei https://doaj.org/toc/2234-7666 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 37 2012 1 34-40
allfields_unstemmed	10.5395/rde.2012.37.1.34 doi (DE-627)DOAJ023978228 (DE-599)DOAJ716e085e92714d5aa29b4fc6f5d070e2 DE-627 ger DE-627 rakwb eng RK1-715 Choo-Ryung Chung verfasserin aut Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation. Dentistry Young-Ju Oh verfasserin aut Ha-Na Kim verfasserin aut Yeul Park verfasserin aut Min-Jeong Kim verfasserin aut Su-Jung Shin verfasserin aut Yoon-Jeong Choi verfasserin aut Kyung-Ho Kim verfasserin aut In Restorative Dentistry & Endodontics Korean Academy of Conservative Dentistry, 2017 37(2012), 1, Seite 34-40 (DE-627)746065167 (DE-600)2715357-5 22347666 nnns volume:37 year:2012 number:1 pages:34-40 https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 kostenfrei https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/toc/2234-7658 Journal toc kostenfrei https://doaj.org/toc/2234-7666 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 37 2012 1 34-40
allfieldsGer	10.5395/rde.2012.37.1.34 doi (DE-627)DOAJ023978228 (DE-599)DOAJ716e085e92714d5aa29b4fc6f5d070e2 DE-627 ger DE-627 rakwb eng RK1-715 Choo-Ryung Chung verfasserin aut Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation. Dentistry Young-Ju Oh verfasserin aut Ha-Na Kim verfasserin aut Yeul Park verfasserin aut Min-Jeong Kim verfasserin aut Su-Jung Shin verfasserin aut Yoon-Jeong Choi verfasserin aut Kyung-Ho Kim verfasserin aut In Restorative Dentistry & Endodontics Korean Academy of Conservative Dentistry, 2017 37(2012), 1, Seite 34-40 (DE-627)746065167 (DE-600)2715357-5 22347666 nnns volume:37 year:2012 number:1 pages:34-40 https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 kostenfrei https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/toc/2234-7658 Journal toc kostenfrei https://doaj.org/toc/2234-7666 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 37 2012 1 34-40
allfieldsSound	10.5395/rde.2012.37.1.34 doi (DE-627)DOAJ023978228 (DE-599)DOAJ716e085e92714d5aa29b4fc6f5d070e2 DE-627 ger DE-627 rakwb eng RK1-715 Choo-Ryung Chung verfasserin aut Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation. Dentistry Young-Ju Oh verfasserin aut Ha-Na Kim verfasserin aut Yeul Park verfasserin aut Min-Jeong Kim verfasserin aut Su-Jung Shin verfasserin aut Yoon-Jeong Choi verfasserin aut Kyung-Ho Kim verfasserin aut In Restorative Dentistry & Endodontics Korean Academy of Conservative Dentistry, 2017 37(2012), 1, Seite 34-40 (DE-627)746065167 (DE-600)2715357-5 22347666 nnns volume:37 year:2012 number:1 pages:34-40 https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 kostenfrei https://doi.org/10.5395/rde.2012.37.1.34 kostenfrei https://doaj.org/toc/2234-7658 Journal toc kostenfrei https://doaj.org/toc/2234-7666 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 37 2012 1 34-40
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authorswithroles_txt_mv	Choo-Ryung Chung @@aut@@ Young-Ju Oh @@aut@@ Ha-Na Kim @@aut@@ Yeul Park @@aut@@ Min-Jeong Kim @@aut@@ Su-Jung Shin @@aut@@ Yoon-Jeong Choi @@aut@@ Kyung-Ho Kim @@aut@@
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Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. 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callnumber-first	R - Medicine
author	Choo-Ryung Chung
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topic_title	RK1-715 Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
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title	Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
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title_full	Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
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author_browse	Choo-Ryung Chung Young-Ju Oh Ha-Na Kim Yeul Park Min-Jeong Kim Su-Jung Shin Yoon-Jeong Choi Kyung-Ho Kim
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title_sort	morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
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title_auth	Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
abstract	Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
abstractGer	Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
abstract_unstemmed	Objectives The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
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title_short	Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
url	https://doi.org/10.5395/rde.2012.37.1.34 https://doaj.org/article/716e085e92714d5aa29b4fc6f5d070e2 https://doaj.org/toc/2234-7658 https://doaj.org/toc/2234-7666
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author2	Young-Ju Oh Ha-Na Kim Yeul Park Min-Jeong Kim Su-Jung Shin Yoon-Jeong Choi Kyung-Ho Kim
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