Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.

OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided...
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Autor*in:	Takamitsu Koga [verfasserIn] Tokutaro Minamizato [verfasserIn] Yosuke Kawai [verfasserIn] Kei-ichiro Miura [verfasserIn] Takashi I [verfasserIn] Yuya Nakatani [verfasserIn] Yoshinori Sumita [verfasserIn] Izumi Asahina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Übergeordnetes Werk:	In: PLoS ONE - Public Library of Science (PLoS), 2007, 11(2016), 1, p e0147235
Übergeordnetes Werk:	volume:11 ; year:2016 ; number:1, p e0147235

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1371/journal.pone.0147235

Katalog-ID:	DOAJ02406677X

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publishDate	2016
allfields	10.1371/journal.pone.0147235 doi (DE-627)DOAJ02406677X (DE-599)DOAJ8ca2263250454389b0df16244a69dce9 DE-627 ger DE-627 rakwb eng Takamitsu Koga verfasserin aut Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size. 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute. Medicine R Science Q Tokutaro Minamizato verfasserin aut Yosuke Kawai verfasserin aut Kei-ichiro Miura verfasserin aut Takashi I verfasserin aut Yuya Nakatani verfasserin aut Yoshinori Sumita verfasserin aut Izumi Asahina verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 11(2016), 1, p e0147235 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:11 year:2016 number:1, p e0147235 https://doi.org/10.1371/journal.pone.0147235 kostenfrei https://doaj.org/article/8ca2263250454389b0df16244a69dce9 kostenfrei http://europepmc.org/articles/PMC4721666?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1, p e0147235
spelling	10.1371/journal.pone.0147235 doi (DE-627)DOAJ02406677X (DE-599)DOAJ8ca2263250454389b0df16244a69dce9 DE-627 ger DE-627 rakwb eng Takamitsu Koga verfasserin aut Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size. 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute. Medicine R Science Q Tokutaro Minamizato verfasserin aut Yosuke Kawai verfasserin aut Kei-ichiro Miura verfasserin aut Takashi I verfasserin aut Yuya Nakatani verfasserin aut Yoshinori Sumita verfasserin aut Izumi Asahina verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 11(2016), 1, p e0147235 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:11 year:2016 number:1, p e0147235 https://doi.org/10.1371/journal.pone.0147235 kostenfrei https://doaj.org/article/8ca2263250454389b0df16244a69dce9 kostenfrei http://europepmc.org/articles/PMC4721666?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1, p e0147235
allfields_unstemmed	10.1371/journal.pone.0147235 doi (DE-627)DOAJ02406677X (DE-599)DOAJ8ca2263250454389b0df16244a69dce9 DE-627 ger DE-627 rakwb eng Takamitsu Koga verfasserin aut Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size. 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute. Medicine R Science Q Tokutaro Minamizato verfasserin aut Yosuke Kawai verfasserin aut Kei-ichiro Miura verfasserin aut Takashi I verfasserin aut Yuya Nakatani verfasserin aut Yoshinori Sumita verfasserin aut Izumi Asahina verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 11(2016), 1, p e0147235 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:11 year:2016 number:1, p e0147235 https://doi.org/10.1371/journal.pone.0147235 kostenfrei https://doaj.org/article/8ca2263250454389b0df16244a69dce9 kostenfrei http://europepmc.org/articles/PMC4721666?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1, p e0147235
allfieldsGer	10.1371/journal.pone.0147235 doi (DE-627)DOAJ02406677X (DE-599)DOAJ8ca2263250454389b0df16244a69dce9 DE-627 ger DE-627 rakwb eng Takamitsu Koga verfasserin aut Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size. 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute. Medicine R Science Q Tokutaro Minamizato verfasserin aut Yosuke Kawai verfasserin aut Kei-ichiro Miura verfasserin aut Takashi I verfasserin aut Yuya Nakatani verfasserin aut Yoshinori Sumita verfasserin aut Izumi Asahina verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 11(2016), 1, p e0147235 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:11 year:2016 number:1, p e0147235 https://doi.org/10.1371/journal.pone.0147235 kostenfrei https://doaj.org/article/8ca2263250454389b0df16244a69dce9 kostenfrei http://europepmc.org/articles/PMC4721666?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1, p e0147235
allfieldsSound	10.1371/journal.pone.0147235 doi (DE-627)DOAJ02406677X (DE-599)DOAJ8ca2263250454389b0df16244a69dce9 DE-627 ger DE-627 rakwb eng Takamitsu Koga verfasserin aut Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size. 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute. Medicine R Science Q Tokutaro Minamizato verfasserin aut Yosuke Kawai verfasserin aut Kei-ichiro Miura verfasserin aut Takashi I verfasserin aut Yuya Nakatani verfasserin aut Yoshinori Sumita verfasserin aut Izumi Asahina verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 11(2016), 1, p e0147235 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:11 year:2016 number:1, p e0147235 https://doi.org/10.1371/journal.pone.0147235 kostenfrei https://doaj.org/article/8ca2263250454389b0df16244a69dce9 kostenfrei http://europepmc.org/articles/PMC4721666?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2016 1, p e0147235
language	English
source	In PLoS ONE 11(2016), 1, p e0147235 volume:11 year:2016 number:1, p e0147235
sourceStr	In PLoS ONE 11(2016), 1, p e0147235 volume:11 year:2016 number:1, p e0147235
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Medicine R Science Q
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container_title	PLoS ONE
authorswithroles_txt_mv	Takamitsu Koga @@aut@@ Tokutaro Minamizato @@aut@@ Yosuke Kawai @@aut@@ Kei-ichiro Miura @@aut@@ Takashi I @@aut@@ Yuya Nakatani @@aut@@ Yoshinori Sumita @@aut@@ Izumi Asahina @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	523574592
id	DOAJ02406677X
language_de	englisch
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author	Takamitsu Koga
spellingShingle	Takamitsu Koga misc Medicine misc R misc Science misc Q Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
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topic_title	Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size
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title	Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
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title_full	Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size
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author_browse	Takamitsu Koga Tokutaro Minamizato Yosuke Kawai Kei-ichiro Miura Takashi I Yuya Nakatani Yoshinori Sumita Izumi Asahina
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title_sort	bone regeneration using dentin matrix depends on the degree of demineralization and particle size
title_auth	Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
abstract	OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute.
abstractGer	OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute.
abstract_unstemmed	OBJECTIVES:This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. MATERIALS AND METHODS:Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. RESULTS:Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. CONCLUSIONS:PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute.
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title_short	Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
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author2	Tokutaro Minamizato Yosuke Kawai Kei-ichiro Miura Takashi I Yuya Nakatani Yoshinori Sumita Izumi Asahina
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