Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon

<p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting s...
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Autor*in:	Lawrence Mark L [verfasserIn] Wills Robert W [verfasserIn] Germon Pierre [verfasserIn] Karsi Attila [verfasserIn] Howe Kevin [verfasserIn] Bailey Richard H [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: BMC Microbiology - BMC, 2003, 10(2010), 1, p 197
Übergeordnetes Werk:	volume:10 ; year:2010 ; number:1, p 197

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2180-10-197

Katalog-ID:	DOAJ024174556

Internformat


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publishDate	2010
allfields	10.1186/1471-2180-10-197 doi (DE-627)DOAJ024174556 (DE-599)DOAJ07363b2ce43a48b987c3152741f3d7c1 DE-627 ger DE-627 rakwb eng QR1-502 Lawrence Mark L verfasserin aut Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p< Microbiology Wills Robert W verfasserin aut Germon Pierre verfasserin aut Karsi Attila verfasserin aut Howe Kevin verfasserin aut Bailey Richard H verfasserin aut In BMC Microbiology BMC, 2003 10(2010), 1, p 197 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:10 year:2010 number:1, p 197 https://doi.org/10.1186/1471-2180-10-197 kostenfrei https://doaj.org/article/07363b2ce43a48b987c3152741f3d7c1 kostenfrei http://www.biomedcentral.com/1471-2180/10/197 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2010 1, p 197
spelling	10.1186/1471-2180-10-197 doi (DE-627)DOAJ024174556 (DE-599)DOAJ07363b2ce43a48b987c3152741f3d7c1 DE-627 ger DE-627 rakwb eng QR1-502 Lawrence Mark L verfasserin aut Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p< Microbiology Wills Robert W verfasserin aut Germon Pierre verfasserin aut Karsi Attila verfasserin aut Howe Kevin verfasserin aut Bailey Richard H verfasserin aut In BMC Microbiology BMC, 2003 10(2010), 1, p 197 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:10 year:2010 number:1, p 197 https://doi.org/10.1186/1471-2180-10-197 kostenfrei https://doaj.org/article/07363b2ce43a48b987c3152741f3d7c1 kostenfrei http://www.biomedcentral.com/1471-2180/10/197 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2010 1, p 197
allfields_unstemmed	10.1186/1471-2180-10-197 doi (DE-627)DOAJ024174556 (DE-599)DOAJ07363b2ce43a48b987c3152741f3d7c1 DE-627 ger DE-627 rakwb eng QR1-502 Lawrence Mark L verfasserin aut Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p< Microbiology Wills Robert W verfasserin aut Germon Pierre verfasserin aut Karsi Attila verfasserin aut Howe Kevin verfasserin aut Bailey Richard H verfasserin aut In BMC Microbiology BMC, 2003 10(2010), 1, p 197 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:10 year:2010 number:1, p 197 https://doi.org/10.1186/1471-2180-10-197 kostenfrei https://doaj.org/article/07363b2ce43a48b987c3152741f3d7c1 kostenfrei http://www.biomedcentral.com/1471-2180/10/197 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2010 1, p 197
allfieldsGer	10.1186/1471-2180-10-197 doi (DE-627)DOAJ024174556 (DE-599)DOAJ07363b2ce43a48b987c3152741f3d7c1 DE-627 ger DE-627 rakwb eng QR1-502 Lawrence Mark L verfasserin aut Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p< Microbiology Wills Robert W verfasserin aut Germon Pierre verfasserin aut Karsi Attila verfasserin aut Howe Kevin verfasserin aut Bailey Richard H verfasserin aut In BMC Microbiology BMC, 2003 10(2010), 1, p 197 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:10 year:2010 number:1, p 197 https://doi.org/10.1186/1471-2180-10-197 kostenfrei https://doaj.org/article/07363b2ce43a48b987c3152741f3d7c1 kostenfrei http://www.biomedcentral.com/1471-2180/10/197 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2010 1, p 197
allfieldsSound	10.1186/1471-2180-10-197 doi (DE-627)DOAJ024174556 (DE-599)DOAJ07363b2ce43a48b987c3152741f3d7c1 DE-627 ger DE-627 rakwb eng QR1-502 Lawrence Mark L verfasserin aut Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p< Microbiology Wills Robert W verfasserin aut Germon Pierre verfasserin aut Karsi Attila verfasserin aut Howe Kevin verfasserin aut Bailey Richard H verfasserin aut In BMC Microbiology BMC, 2003 10(2010), 1, p 197 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:10 year:2010 number:1, p 197 https://doi.org/10.1186/1471-2180-10-197 kostenfrei https://doaj.org/article/07363b2ce43a48b987c3152741f3d7c1 kostenfrei http://www.biomedcentral.com/1471-2180/10/197 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2010 1, p 197
language	English
source	In BMC Microbiology 10(2010), 1, p 197 volume:10 year:2010 number:1, p 197
sourceStr	In BMC Microbiology 10(2010), 1, p 197 volume:10 year:2010 number:1, p 197
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microbiology
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container_title	BMC Microbiology
authorswithroles_txt_mv	Lawrence Mark L @@aut@@ Wills Robert W @@aut@@ Germon Pierre @@aut@@ Karsi Attila @@aut@@ Howe Kevin @@aut@@ Bailey Richard H @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	326644997
id	DOAJ024174556
language_de	englisch
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callnumber-first	Q - Science
author	Lawrence Mark L
spellingShingle	Lawrence Mark L misc QR1-502 misc Microbiology Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
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topic_title	QR1-502 Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
topic	misc QR1-502 misc Microbiology
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title	Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
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title_full	Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
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author_browse	Lawrence Mark L Wills Robert W Germon Pierre Karsi Attila Howe Kevin Bailey Richard H
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doi_str_mv	10.1186/1471-2180-10-197
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title_sort	development of stable reporter system cloning <it<luxcdabe </it<genes into chromosome of <it<salmonella enterica </it<serotypes using tn7 transposon
callnumber	QR1-502
title_auth	Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
abstract	<p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent <it<Salmonella enterica </it<serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of <it<luxCDABE </it<genes into the chromosome of eleven <it<Salmonella enterica </it<serotypes isolated from the broiler production continuum.</p< <p<Results</p< <p<We found that the <it<lux </it<operon is constitutively expressed from the chromosome post-transposition and the <it<lux </it<cassette is stable without external pressure, i.e. antibiotic selection, for all <it<Salmonella enterica </it<serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies.</p< <p<Conclusions</p< <p<This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for <it<Salmonella </it<on food products in real-time for both short and long term studies.</p<
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title_short	Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon
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Development of stable reporter system cloning <it<luxCDABE </it<genes into chromosome of <it<Salmonella enterica </it<serotypes using Tn7 transposon

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