Extracellular Vesicle Flow Cytometry Analysis and Standardization

The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to acc...
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Autor*in:	Joshua A. Welsh [verfasserIn] Judith A. Holloway [verfasserIn] James S. Wilkinson [verfasserIn] Nicola A. Englyst [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization

Übergeordnetes Werk:	In: Frontiers in Cell and Developmental Biology - Frontiers Media S.A., 2014, 5(2017)
Übergeordnetes Werk:	volume:5 ; year:2017

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcell.2017.00078

Katalog-ID:	DOAJ024260118

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520			\|a The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.
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allfields	10.3389/fcell.2017.00078 doi (DE-627)DOAJ024260118 (DE-599)DOAJdc699a9e7d2441d5b0a35942c9061926 DE-627 ger DE-627 rakwb eng QH301-705.5 Joshua A. Welsh verfasserin aut Extracellular Vesicle Flow Cytometry Analysis and Standardization 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics. extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General) Judith A. Holloway verfasserin aut James S. Wilkinson verfasserin aut Nicola A. Englyst verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 5(2017) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:5 year:2017 https://doi.org/10.3389/fcell.2017.00078 kostenfrei https://doaj.org/article/dc699a9e7d2441d5b0a35942c9061926 kostenfrei http://journal.frontiersin.org/article/10.3389/fcell.2017.00078/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
spelling	10.3389/fcell.2017.00078 doi (DE-627)DOAJ024260118 (DE-599)DOAJdc699a9e7d2441d5b0a35942c9061926 DE-627 ger DE-627 rakwb eng QH301-705.5 Joshua A. Welsh verfasserin aut Extracellular Vesicle Flow Cytometry Analysis and Standardization 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics. extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General) Judith A. Holloway verfasserin aut James S. Wilkinson verfasserin aut Nicola A. Englyst verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 5(2017) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:5 year:2017 https://doi.org/10.3389/fcell.2017.00078 kostenfrei https://doaj.org/article/dc699a9e7d2441d5b0a35942c9061926 kostenfrei http://journal.frontiersin.org/article/10.3389/fcell.2017.00078/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
allfields_unstemmed	10.3389/fcell.2017.00078 doi (DE-627)DOAJ024260118 (DE-599)DOAJdc699a9e7d2441d5b0a35942c9061926 DE-627 ger DE-627 rakwb eng QH301-705.5 Joshua A. Welsh verfasserin aut Extracellular Vesicle Flow Cytometry Analysis and Standardization 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics. extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General) Judith A. Holloway verfasserin aut James S. Wilkinson verfasserin aut Nicola A. Englyst verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 5(2017) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:5 year:2017 https://doi.org/10.3389/fcell.2017.00078 kostenfrei https://doaj.org/article/dc699a9e7d2441d5b0a35942c9061926 kostenfrei http://journal.frontiersin.org/article/10.3389/fcell.2017.00078/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
allfieldsGer	10.3389/fcell.2017.00078 doi (DE-627)DOAJ024260118 (DE-599)DOAJdc699a9e7d2441d5b0a35942c9061926 DE-627 ger DE-627 rakwb eng QH301-705.5 Joshua A. Welsh verfasserin aut Extracellular Vesicle Flow Cytometry Analysis and Standardization 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics. extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General) Judith A. Holloway verfasserin aut James S. Wilkinson verfasserin aut Nicola A. Englyst verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 5(2017) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:5 year:2017 https://doi.org/10.3389/fcell.2017.00078 kostenfrei https://doaj.org/article/dc699a9e7d2441d5b0a35942c9061926 kostenfrei http://journal.frontiersin.org/article/10.3389/fcell.2017.00078/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
allfieldsSound	10.3389/fcell.2017.00078 doi (DE-627)DOAJ024260118 (DE-599)DOAJdc699a9e7d2441d5b0a35942c9061926 DE-627 ger DE-627 rakwb eng QH301-705.5 Joshua A. Welsh verfasserin aut Extracellular Vesicle Flow Cytometry Analysis and Standardization 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics. extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General) Judith A. Holloway verfasserin aut James S. Wilkinson verfasserin aut Nicola A. Englyst verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 5(2017) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:5 year:2017 https://doi.org/10.3389/fcell.2017.00078 kostenfrei https://doaj.org/article/dc699a9e7d2441d5b0a35942c9061926 kostenfrei http://journal.frontiersin.org/article/10.3389/fcell.2017.00078/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
language	English
source	In Frontiers in Cell and Developmental Biology 5(2017) volume:5 year:2017
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topic_facet	extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization Biology (General)
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container_title	Frontiers in Cell and Developmental Biology
authorswithroles_txt_mv	Joshua A. Welsh @@aut@@ Judith A. Holloway @@aut@@ James S. Wilkinson @@aut@@ Nicola A. Englyst @@aut@@
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author	Joshua A. Welsh
spellingShingle	Joshua A. Welsh misc QH301-705.5 misc extracellular vesicles misc EV misc Extracellular vesicles (EVs) misc flow cytometry (FCM) misc scattering misc fluorescence standardization misc Biology (General) Extracellular Vesicle Flow Cytometry Analysis and Standardization
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topic_title	QH301-705.5 Extracellular Vesicle Flow Cytometry Analysis and Standardization extracellular vesicles EV Extracellular vesicles (EVs) flow cytometry (FCM) scattering fluorescence standardization
topic	misc QH301-705.5 misc extracellular vesicles misc EV misc Extracellular vesicles (EVs) misc flow cytometry (FCM) misc scattering misc fluorescence standardization misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc extracellular vesicles misc EV misc Extracellular vesicles (EVs) misc flow cytometry (FCM) misc scattering misc fluorescence standardization misc Biology (General)
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title	Extracellular Vesicle Flow Cytometry Analysis and Standardization
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title_full	Extracellular Vesicle Flow Cytometry Analysis and Standardization
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title_auth	Extracellular Vesicle Flow Cytometry Analysis and Standardization
abstract	The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.
abstractGer	The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.
abstract_unstemmed	The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.
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title_short	Extracellular Vesicle Flow Cytometry Analysis and Standardization
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Extracellular Vesicle Flow Cytometry Analysis and Standardization

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