In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products

Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodolo...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Felipe Machado Trombete [verfasserIn] Thaís Barbosa Santos [verfasserIn] Glória Maria Direito [verfasserIn] Marcelo Elias Fraga [verfasserIn] Tatiana Saldanha [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2014

Schlagwörter:	Triticum aestivum L. Aspergillus mycotoxins wheat bran whole wheat flour

Übergeordnetes Werk:	In: Pesquisa Agropecuária Tropical - Universidade Federal de Goiás, 2010, 44(2014), 3, Seite 255-262
Übergeordnetes Werk:	volume:44 ; year:2014 ; number:3 ; pages:255-262

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/S1983-40632014000300003

Katalog-ID:	DOAJ024277568

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callnumber-first	S - Agriculture
author	Felipe Machado Trombete
spellingShingle	Felipe Machado Trombete misc S1-972 misc Triticum aestivum L. misc Aspergillus misc mycotoxins misc wheat bran misc whole wheat flour misc Agriculture (General) In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
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topic_title	S1-972 In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products Triticum aestivum L. Aspergillus mycotoxins wheat bran whole wheat flour
topic	misc S1-972 misc Triticum aestivum L. misc Aspergillus misc mycotoxins misc wheat bran misc whole wheat flour misc Agriculture (General)
topic_unstemmed	misc S1-972 misc Triticum aestivum L. misc Aspergillus misc mycotoxins misc wheat bran misc whole wheat flour misc Agriculture (General)
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title	In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
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title_full	In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
author_sort	Felipe Machado Trombete
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title_sort	in-house validation of a method for determining aflatoxins b1, b2, g1 and g2 in wheat and wheat by-products
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title_auth	In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
abstract	Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.
abstractGer	Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.
abstract_unstemmed	Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.
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title_short	In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
url	https://doi.org/10.1590/S1983-40632014000300003 https://doaj.org/article/77eeb434035c4a12af3123292b998ec6 https://www.revistas.ufg.br/pat/article/view/25449 https://doaj.org/toc/1983-4063
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author2	Thaís Barbosa Santos Glória Maria Direito Marcelo Elias Fraga Tatiana Saldanha
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up_date	2024-07-03T22:09:15.269Z
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In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products

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