Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay

Plasmidic AmpC (pAmpC) enzymes are responsible for the hydrolysis of extended-spectrum cephalosporins but they are not routinely investigated in many clinical laboratories. Phenotypic assays, currently the reference methods, are cumbersome and culture dependent. These methods compare the activity of...
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Autor*in:	Inês Martins-Oliveira [verfasserIn] Blanca Pérez-Viso [verfasserIn] Ana Silva-Dias [verfasserIn] Rosário Gomes [verfasserIn] Luísa Peixe [verfasserIn] Ângela Novais [verfasserIn] Rafael Cantón [verfasserIn] Cidália Pina-Vaz [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	pAmpC flow cytometry resistance mechanisms rapid antimicrobial susceptibility testing

Übergeordnetes Werk:	In: Antibiotics - MDPI AG, 2013, 11(2022), 8, p 1130
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:8, p 1130

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/antibiotics11081130

Katalog-ID:	DOAJ024470767

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topic_facet	pAmpC flow cytometry resistance mechanisms rapid antimicrobial susceptibility testing Therapeutics. Pharmacology
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authorswithroles_txt_mv	Inês Martins-Oliveira @@aut@@ Blanca Pérez-Viso @@aut@@ Ana Silva-Dias @@aut@@ Rosário Gomes @@aut@@ Luísa Peixe @@aut@@ Ângela Novais @@aut@@ Rafael Cantón @@aut@@ Cidália Pina-Vaz @@aut@@
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callnumber-first	R - Medicine
author	Inês Martins-Oliveira
spellingShingle	Inês Martins-Oliveira misc RM1-950 misc pAmpC misc flow cytometry misc resistance mechanisms misc rapid antimicrobial susceptibility testing misc Therapeutics. Pharmacology Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay
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topic_title	RM1-950 Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay pAmpC flow cytometry resistance mechanisms rapid antimicrobial susceptibility testing
topic	misc RM1-950 misc pAmpC misc flow cytometry misc resistance mechanisms misc rapid antimicrobial susceptibility testing misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc pAmpC misc flow cytometry misc resistance mechanisms misc rapid antimicrobial susceptibility testing misc Therapeutics. Pharmacology
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title	Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay
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title_full	Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay
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title_sort	rapid detection of plasmid ampc beta-lactamases by a flow cytometry assay
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title_auth	Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay
abstract	Plasmidic AmpC (pAmpC) enzymes are responsible for the hydrolysis of extended-spectrum cephalosporins but they are not routinely investigated in many clinical laboratories. Phenotypic assays, currently the reference methods, are cumbersome and culture dependent. These methods compare the activity of cephalosporins with and without class C inhibitors and the results are provided in 24–48 h. Detection by molecular methods is quicker, but several genes should be investigated. A new assay for the rapid phenotypic detection of pAmpC enzymes of the <i<Enterobacterales</i< group-I (not usually AmpC producers) based on flow cytometry technology was developed and validated. The technology was evaluated in two sites: FASTinov, a spin-off of Porto University (Portugal) where the technology was developed, and the Microbiology Department of Ramón y Cajal University Hospital in Madrid (Spain). A total of 100 strains were phenotypically screened by disk diffusion for the pAmpC with the new 2 h assay. Molecular detection of the pAmpC genes was also performed on discrepant results. Forty-two percent of the strains were phenotypically classified as pAmpC producers using disk diffusion. The percentage of agreement of the flow cytometric assay was 93.0%, with 95.5% sensitivity and 91.1% specificity. Our proposed rapid assay based on flow cytometry technology can, in two hours, accurately detect pAmpC enzymes.
abstractGer	Plasmidic AmpC (pAmpC) enzymes are responsible for the hydrolysis of extended-spectrum cephalosporins but they are not routinely investigated in many clinical laboratories. Phenotypic assays, currently the reference methods, are cumbersome and culture dependent. These methods compare the activity of cephalosporins with and without class C inhibitors and the results are provided in 24–48 h. Detection by molecular methods is quicker, but several genes should be investigated. A new assay for the rapid phenotypic detection of pAmpC enzymes of the <i<Enterobacterales</i< group-I (not usually AmpC producers) based on flow cytometry technology was developed and validated. The technology was evaluated in two sites: FASTinov, a spin-off of Porto University (Portugal) where the technology was developed, and the Microbiology Department of Ramón y Cajal University Hospital in Madrid (Spain). A total of 100 strains were phenotypically screened by disk diffusion for the pAmpC with the new 2 h assay. Molecular detection of the pAmpC genes was also performed on discrepant results. Forty-two percent of the strains were phenotypically classified as pAmpC producers using disk diffusion. The percentage of agreement of the flow cytometric assay was 93.0%, with 95.5% sensitivity and 91.1% specificity. Our proposed rapid assay based on flow cytometry technology can, in two hours, accurately detect pAmpC enzymes.
abstract_unstemmed	Plasmidic AmpC (pAmpC) enzymes are responsible for the hydrolysis of extended-spectrum cephalosporins but they are not routinely investigated in many clinical laboratories. Phenotypic assays, currently the reference methods, are cumbersome and culture dependent. These methods compare the activity of cephalosporins with and without class C inhibitors and the results are provided in 24–48 h. Detection by molecular methods is quicker, but several genes should be investigated. A new assay for the rapid phenotypic detection of pAmpC enzymes of the <i<Enterobacterales</i< group-I (not usually AmpC producers) based on flow cytometry technology was developed and validated. The technology was evaluated in two sites: FASTinov, a spin-off of Porto University (Portugal) where the technology was developed, and the Microbiology Department of Ramón y Cajal University Hospital in Madrid (Spain). A total of 100 strains were phenotypically screened by disk diffusion for the pAmpC with the new 2 h assay. Molecular detection of the pAmpC genes was also performed on discrepant results. Forty-two percent of the strains were phenotypically classified as pAmpC producers using disk diffusion. The percentage of agreement of the flow cytometric assay was 93.0%, with 95.5% sensitivity and 91.1% specificity. Our proposed rapid assay based on flow cytometry technology can, in two hours, accurately detect pAmpC enzymes.
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title_short	Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay
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author2	Blanca Pérez-Viso Ana Silva-Dias Rosário Gomes Luísa Peixe Ângela Novais Rafael Cantón Cidália Pina-Vaz
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up_date	2024-07-03T23:04:47.878Z
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Rapid Detection of Plasmid AmpC Beta-Lactamases by a Flow Cytometry Assay

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