Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape

Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-speci...
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Autor*in:	Roberta Marzi [verfasserIn] Jessica Bassi [verfasserIn] Chiara Silacci-Fregni [verfasserIn] Istvan Bartha [verfasserIn] Francesco Muoio [verfasserIn] Katja Culap [verfasserIn] Nicole Sprugasci [verfasserIn] Gloria Lombardo [verfasserIn] Christian Saliba [verfasserIn] Elisabetta Cameroni [verfasserIn] Antonino Cassotta [verfasserIn] Jun Siong Low [verfasserIn] Alexandra C. Walls [verfasserIn] Matthew McCallum [verfasserIn] M. Alejandra Tortorici [verfasserIn] John E. Bowen [verfasserIn] Exequiel A. Dellota, Jr. [verfasserIn] Josh R. Dillen [verfasserIn] Nadine Czudnochowski [verfasserIn] Laura Pertusini [verfasserIn] Tatiana Terrot [verfasserIn] Valentino Lepori [verfasserIn] Maciej Tarkowski [verfasserIn] Agostino Riva [verfasserIn] Maira Biggiogero [verfasserIn] Alessandra Franzetti-Pellanda [verfasserIn] Christian Garzoni [verfasserIn] Paolo Ferrari [verfasserIn] Alessandro Ceschi [verfasserIn] Olivier Giannini [verfasserIn] Colin Havenar-Daughton [verfasserIn] Amalio Telenti [verfasserIn] Ann Arvin [verfasserIn] Herbert W. Virgin [verfasserIn] Federica Sallusto [verfasserIn] David Veesler [verfasserIn] Antonio Lanzavecchia [verfasserIn] Davide Corti [verfasserIn] Luca Piccoli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Immunology Virology

Übergeordnetes Werk:	In: iScience - Elsevier, 2019, 26(2023), 1, Seite 105726-
Übergeordnetes Werk:	volume:26 ; year:2023 ; number:1 ; pages:105726-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.isci.2022.105726

Katalog-ID:	DOAJ024639141

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245	1	0	\|a Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape
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520			\|a Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants.
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allfields	10.1016/j.isci.2022.105726 doi (DE-627)DOAJ024639141 (DE-599)DOAJbf76fb519d3e40cd9db93e017e602507 DE-627 ger DE-627 rakwb eng Roberta Marzi verfasserin aut Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants. Immunology Virology Science Q Jessica Bassi verfasserin aut Chiara Silacci-Fregni verfasserin aut Istvan Bartha verfasserin aut Francesco Muoio verfasserin aut Katja Culap verfasserin aut Nicole Sprugasci verfasserin aut Gloria Lombardo verfasserin aut Christian Saliba verfasserin aut Elisabetta Cameroni verfasserin aut Antonino Cassotta verfasserin aut Jun Siong Low verfasserin aut Alexandra C. Walls verfasserin aut Matthew McCallum verfasserin aut M. Alejandra Tortorici verfasserin aut John E. Bowen verfasserin aut Exequiel A. Dellota, Jr. verfasserin aut Josh R. Dillen verfasserin aut Nadine Czudnochowski verfasserin aut Laura Pertusini verfasserin aut Tatiana Terrot verfasserin aut Valentino Lepori verfasserin aut Maciej Tarkowski verfasserin aut Agostino Riva verfasserin aut Maira Biggiogero verfasserin aut Alessandra Franzetti-Pellanda verfasserin aut Christian Garzoni verfasserin aut Paolo Ferrari verfasserin aut Alessandro Ceschi verfasserin aut Olivier Giannini verfasserin aut Colin Havenar-Daughton verfasserin aut Amalio Telenti verfasserin aut Ann Arvin verfasserin aut Herbert W. Virgin verfasserin aut Federica Sallusto verfasserin aut David Veesler verfasserin aut Antonio Lanzavecchia verfasserin aut Davide Corti verfasserin aut Luca Piccoli verfasserin aut In iScience Elsevier, 2019 26(2023), 1, Seite 105726- (DE-627)1019532106 25890042 nnns volume:26 year:2023 number:1 pages:105726- https://doi.org/10.1016/j.isci.2022.105726 kostenfrei https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 kostenfrei http://www.sciencedirect.com/science/article/pii/S258900422201999X kostenfrei https://doaj.org/toc/2589-0042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 26 2023 1 105726-
spelling	10.1016/j.isci.2022.105726 doi (DE-627)DOAJ024639141 (DE-599)DOAJbf76fb519d3e40cd9db93e017e602507 DE-627 ger DE-627 rakwb eng Roberta Marzi verfasserin aut Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants. Immunology Virology Science Q Jessica Bassi verfasserin aut Chiara Silacci-Fregni verfasserin aut Istvan Bartha verfasserin aut Francesco Muoio verfasserin aut Katja Culap verfasserin aut Nicole Sprugasci verfasserin aut Gloria Lombardo verfasserin aut Christian Saliba verfasserin aut Elisabetta Cameroni verfasserin aut Antonino Cassotta verfasserin aut Jun Siong Low verfasserin aut Alexandra C. Walls verfasserin aut Matthew McCallum verfasserin aut M. Alejandra Tortorici verfasserin aut John E. Bowen verfasserin aut Exequiel A. Dellota, Jr. verfasserin aut Josh R. Dillen verfasserin aut Nadine Czudnochowski verfasserin aut Laura Pertusini verfasserin aut Tatiana Terrot verfasserin aut Valentino Lepori verfasserin aut Maciej Tarkowski verfasserin aut Agostino Riva verfasserin aut Maira Biggiogero verfasserin aut Alessandra Franzetti-Pellanda verfasserin aut Christian Garzoni verfasserin aut Paolo Ferrari verfasserin aut Alessandro Ceschi verfasserin aut Olivier Giannini verfasserin aut Colin Havenar-Daughton verfasserin aut Amalio Telenti verfasserin aut Ann Arvin verfasserin aut Herbert W. Virgin verfasserin aut Federica Sallusto verfasserin aut David Veesler verfasserin aut Antonio Lanzavecchia verfasserin aut Davide Corti verfasserin aut Luca Piccoli verfasserin aut In iScience Elsevier, 2019 26(2023), 1, Seite 105726- (DE-627)1019532106 25890042 nnns volume:26 year:2023 number:1 pages:105726- https://doi.org/10.1016/j.isci.2022.105726 kostenfrei https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 kostenfrei http://www.sciencedirect.com/science/article/pii/S258900422201999X kostenfrei https://doaj.org/toc/2589-0042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 26 2023 1 105726-
allfields_unstemmed	10.1016/j.isci.2022.105726 doi (DE-627)DOAJ024639141 (DE-599)DOAJbf76fb519d3e40cd9db93e017e602507 DE-627 ger DE-627 rakwb eng Roberta Marzi verfasserin aut Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants. Immunology Virology Science Q Jessica Bassi verfasserin aut Chiara Silacci-Fregni verfasserin aut Istvan Bartha verfasserin aut Francesco Muoio verfasserin aut Katja Culap verfasserin aut Nicole Sprugasci verfasserin aut Gloria Lombardo verfasserin aut Christian Saliba verfasserin aut Elisabetta Cameroni verfasserin aut Antonino Cassotta verfasserin aut Jun Siong Low verfasserin aut Alexandra C. Walls verfasserin aut Matthew McCallum verfasserin aut M. Alejandra Tortorici verfasserin aut John E. Bowen verfasserin aut Exequiel A. Dellota, Jr. verfasserin aut Josh R. Dillen verfasserin aut Nadine Czudnochowski verfasserin aut Laura Pertusini verfasserin aut Tatiana Terrot verfasserin aut Valentino Lepori verfasserin aut Maciej Tarkowski verfasserin aut Agostino Riva verfasserin aut Maira Biggiogero verfasserin aut Alessandra Franzetti-Pellanda verfasserin aut Christian Garzoni verfasserin aut Paolo Ferrari verfasserin aut Alessandro Ceschi verfasserin aut Olivier Giannini verfasserin aut Colin Havenar-Daughton verfasserin aut Amalio Telenti verfasserin aut Ann Arvin verfasserin aut Herbert W. Virgin verfasserin aut Federica Sallusto verfasserin aut David Veesler verfasserin aut Antonio Lanzavecchia verfasserin aut Davide Corti verfasserin aut Luca Piccoli verfasserin aut In iScience Elsevier, 2019 26(2023), 1, Seite 105726- (DE-627)1019532106 25890042 nnns volume:26 year:2023 number:1 pages:105726- https://doi.org/10.1016/j.isci.2022.105726 kostenfrei https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 kostenfrei http://www.sciencedirect.com/science/article/pii/S258900422201999X kostenfrei https://doaj.org/toc/2589-0042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 26 2023 1 105726-
allfieldsGer	10.1016/j.isci.2022.105726 doi (DE-627)DOAJ024639141 (DE-599)DOAJbf76fb519d3e40cd9db93e017e602507 DE-627 ger DE-627 rakwb eng Roberta Marzi verfasserin aut Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants. Immunology Virology Science Q Jessica Bassi verfasserin aut Chiara Silacci-Fregni verfasserin aut Istvan Bartha verfasserin aut Francesco Muoio verfasserin aut Katja Culap verfasserin aut Nicole Sprugasci verfasserin aut Gloria Lombardo verfasserin aut Christian Saliba verfasserin aut Elisabetta Cameroni verfasserin aut Antonino Cassotta verfasserin aut Jun Siong Low verfasserin aut Alexandra C. Walls verfasserin aut Matthew McCallum verfasserin aut M. Alejandra Tortorici verfasserin aut John E. Bowen verfasserin aut Exequiel A. Dellota, Jr. verfasserin aut Josh R. Dillen verfasserin aut Nadine Czudnochowski verfasserin aut Laura Pertusini verfasserin aut Tatiana Terrot verfasserin aut Valentino Lepori verfasserin aut Maciej Tarkowski verfasserin aut Agostino Riva verfasserin aut Maira Biggiogero verfasserin aut Alessandra Franzetti-Pellanda verfasserin aut Christian Garzoni verfasserin aut Paolo Ferrari verfasserin aut Alessandro Ceschi verfasserin aut Olivier Giannini verfasserin aut Colin Havenar-Daughton verfasserin aut Amalio Telenti verfasserin aut Ann Arvin verfasserin aut Herbert W. Virgin verfasserin aut Federica Sallusto verfasserin aut David Veesler verfasserin aut Antonio Lanzavecchia verfasserin aut Davide Corti verfasserin aut Luca Piccoli verfasserin aut In iScience Elsevier, 2019 26(2023), 1, Seite 105726- (DE-627)1019532106 25890042 nnns volume:26 year:2023 number:1 pages:105726- https://doi.org/10.1016/j.isci.2022.105726 kostenfrei https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 kostenfrei http://www.sciencedirect.com/science/article/pii/S258900422201999X kostenfrei https://doaj.org/toc/2589-0042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 26 2023 1 105726-
allfieldsSound	10.1016/j.isci.2022.105726 doi (DE-627)DOAJ024639141 (DE-599)DOAJbf76fb519d3e40cd9db93e017e602507 DE-627 ger DE-627 rakwb eng Roberta Marzi verfasserin aut Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants. Immunology Virology Science Q Jessica Bassi verfasserin aut Chiara Silacci-Fregni verfasserin aut Istvan Bartha verfasserin aut Francesco Muoio verfasserin aut Katja Culap verfasserin aut Nicole Sprugasci verfasserin aut Gloria Lombardo verfasserin aut Christian Saliba verfasserin aut Elisabetta Cameroni verfasserin aut Antonino Cassotta verfasserin aut Jun Siong Low verfasserin aut Alexandra C. Walls verfasserin aut Matthew McCallum verfasserin aut M. Alejandra Tortorici verfasserin aut John E. Bowen verfasserin aut Exequiel A. Dellota, Jr. verfasserin aut Josh R. Dillen verfasserin aut Nadine Czudnochowski verfasserin aut Laura Pertusini verfasserin aut Tatiana Terrot verfasserin aut Valentino Lepori verfasserin aut Maciej Tarkowski verfasserin aut Agostino Riva verfasserin aut Maira Biggiogero verfasserin aut Alessandra Franzetti-Pellanda verfasserin aut Christian Garzoni verfasserin aut Paolo Ferrari verfasserin aut Alessandro Ceschi verfasserin aut Olivier Giannini verfasserin aut Colin Havenar-Daughton verfasserin aut Amalio Telenti verfasserin aut Ann Arvin verfasserin aut Herbert W. Virgin verfasserin aut Federica Sallusto verfasserin aut David Veesler verfasserin aut Antonio Lanzavecchia verfasserin aut Davide Corti verfasserin aut Luca Piccoli verfasserin aut In iScience Elsevier, 2019 26(2023), 1, Seite 105726- (DE-627)1019532106 25890042 nnns volume:26 year:2023 number:1 pages:105726- https://doi.org/10.1016/j.isci.2022.105726 kostenfrei https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 kostenfrei http://www.sciencedirect.com/science/article/pii/S258900422201999X kostenfrei https://doaj.org/toc/2589-0042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 26 2023 1 105726-
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source	In iScience 26(2023), 1, Seite 105726- volume:26 year:2023 number:1 pages:105726-
sourceStr	In iScience 26(2023), 1, Seite 105726- volume:26 year:2023 number:1 pages:105726-
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authorswithroles_txt_mv	Roberta Marzi @@aut@@ Jessica Bassi @@aut@@ Chiara Silacci-Fregni @@aut@@ Istvan Bartha @@aut@@ Francesco Muoio @@aut@@ Katja Culap @@aut@@ Nicole Sprugasci @@aut@@ Gloria Lombardo @@aut@@ Christian Saliba @@aut@@ Elisabetta Cameroni @@aut@@ Antonino Cassotta @@aut@@ Jun Siong Low @@aut@@ Alexandra C. Walls @@aut@@ Matthew McCallum @@aut@@ M. Alejandra Tortorici @@aut@@ John E. Bowen @@aut@@ Exequiel A. Dellota, Jr. @@aut@@ Josh R. Dillen @@aut@@ Nadine Czudnochowski @@aut@@ Laura Pertusini @@aut@@ Tatiana Terrot @@aut@@ Valentino Lepori @@aut@@ Maciej Tarkowski @@aut@@ Agostino Riva @@aut@@ Maira Biggiogero @@aut@@ Alessandra Franzetti-Pellanda @@aut@@ Christian Garzoni @@aut@@ Paolo Ferrari @@aut@@ Alessandro Ceschi @@aut@@ Olivier Giannini @@aut@@ Colin Havenar-Daughton @@aut@@ Amalio Telenti @@aut@@ Ann Arvin @@aut@@ Herbert W. Virgin @@aut@@ Federica Sallusto @@aut@@ David Veesler @@aut@@ Antonio Lanzavecchia @@aut@@ Davide Corti @@aut@@ Luca Piccoli @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	1019532106
id	DOAJ024639141
language_de	englisch
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author	Roberta Marzi
spellingShingle	Roberta Marzi misc Immunology misc Virology misc Science misc Q Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape
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topic_title	Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape Immunology Virology
topic	misc Immunology misc Virology misc Science misc Q
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author_browse	Roberta Marzi Jessica Bassi Chiara Silacci-Fregni Istvan Bartha Francesco Muoio Katja Culap Nicole Sprugasci Gloria Lombardo Christian Saliba Elisabetta Cameroni Antonino Cassotta Jun Siong Low Alexandra C. Walls Matthew McCallum M. Alejandra Tortorici John E. Bowen Exequiel A. Dellota, Jr. Josh R. Dillen Nadine Czudnochowski Laura Pertusini Tatiana Terrot Valentino Lepori Maciej Tarkowski Agostino Riva Maira Biggiogero Alessandra Franzetti-Pellanda Christian Garzoni Paolo Ferrari Alessandro Ceschi Olivier Giannini Colin Havenar-Daughton Amalio Telenti Ann Arvin Herbert W. Virgin Federica Sallusto David Veesler Antonio Lanzavecchia Davide Corti Luca Piccoli
container_volume	26
format_se	Elektronische Aufsätze
author-letter	Roberta Marzi
doi_str_mv	10.1016/j.isci.2022.105726
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title_sort	maturation of sars-cov-2 spike-specific memory b cells drives resilience to viral escape
title_auth	Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape
abstract	Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants.
abstractGer	Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants.
abstract_unstemmed	Summary: Memory B cells (MBCs) generate rapid antibody responses upon secondary encounter with a pathogen. Here, we investigated the kinetics, avidity, and cross-reactivity of serum antibodies and MBCs in 155 SARS-CoV-2 infected and vaccinated individuals over a 16-month time frame. SARS-CoV-2-specific MBCs and serum antibodies reached steady-state titers with comparable kinetics in infected and vaccinated individuals. Whereas MBCs of infected individuals targeted both prefusion and postfusion Spike (S), most vaccine-elicited MBCs were specific for prefusion S, consistent with the use of prefusion-stabilized S in mRNA vaccines. Furthermore, a large fraction of MBCs recognizing postfusion S cross-reacted with human betacoronaviruses. The avidity of MBC-derived and serum antibodies increased over time resulting in enhanced resilience to viral escape by SARS-CoV-2 variants, including Omicron BA.1 and BA.2 sublineages, albeit only partially for BA.4 and BA.5 sublineages. Overall, the maturation of high-affinity and broadly reactive MBCs provides the basis for effective recall responses to future SARS-CoV-2 variants.
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title_short	Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape
url	https://doi.org/10.1016/j.isci.2022.105726 https://doaj.org/article/bf76fb519d3e40cd9db93e017e602507 http://www.sciencedirect.com/science/article/pii/S258900422201999X https://doaj.org/toc/2589-0042
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author2	Jessica Bassi Chiara Silacci-Fregni Istvan Bartha Francesco Muoio Katja Culap Nicole Sprugasci Gloria Lombardo Christian Saliba Elisabetta Cameroni Antonino Cassotta Jun Siong Low Alexandra C. Walls Matthew McCallum M. Alejandra Tortorici John E. Bowen Exequiel A. Dellota, Jr. Josh R. Dillen Nadine Czudnochowski Laura Pertusini Tatiana Terrot Valentino Lepori Maciej Tarkowski Agostino Riva Maira Biggiogero Alessandra Franzetti-Pellanda Christian Garzoni Paolo Ferrari Alessandro Ceschi Olivier Giannini Colin Havenar-Daughton Amalio Telenti Ann Arvin Herbert W. Virgin Federica Sallusto David Veesler Antonio Lanzavecchia Davide Corti Luca Piccoli
author2Str	Jessica Bassi Chiara Silacci-Fregni Istvan Bartha Francesco Muoio Katja Culap Nicole Sprugasci Gloria Lombardo Christian Saliba Elisabetta Cameroni Antonino Cassotta Jun Siong Low Alexandra C. Walls Matthew McCallum M. Alejandra Tortorici John E. Bowen Exequiel A. Dellota, Jr. Josh R. Dillen Nadine Czudnochowski Laura Pertusini Tatiana Terrot Valentino Lepori Maciej Tarkowski Agostino Riva Maira Biggiogero Alessandra Franzetti-Pellanda Christian Garzoni Paolo Ferrari Alessandro Ceschi Olivier Giannini Colin Havenar-Daughton Amalio Telenti Ann Arvin Herbert W. Virgin Federica Sallusto David Veesler Antonio Lanzavecchia Davide Corti Luca Piccoli
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doi_str	10.1016/j.isci.2022.105726
up_date	2024-07-03T23:48:59.797Z
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