Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening

We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy....
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Julianna Kobolak [verfasserIn] Annamaria Teglasi [verfasserIn] Tamas Bellak [verfasserIn] Zofia Janstova [verfasserIn] Kinga Molnar [verfasserIn] Melinda Zana [verfasserIn] Istvan Bock [verfasserIn] Lajos Laszlo [verfasserIn] Andras Dinnyes [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	induced pluripotent stem cells neurospheres 3D culture neurite outgrowth neurotoxicity

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 9(2020), 5, p 1122
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:5, p 1122

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells9051122

Katalog-ID:	DOAJ024659118

Internformat


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allfields	10.3390/cells9051122 doi (DE-627)DOAJ024659118 (DE-599)DOAJf73204f0bcb143eb8ba1c965ac7f9b1f DE-627 ger DE-627 rakwb eng QH573-671 Julianna Kobolak verfasserin aut Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional activity of major developmental, structural, and cell-type-specific markers was investigated at weekly intervals to present the differentiation of neurons, astrocytes, and oligodendrocytes. Neurospheres were exposed to different well-known toxicants with or without neurotoxic effect (e.g., paraquat, acrylamide, or ibuprofen) and examined at various stages of the differentiation with an ATP-based cell viability assay optimized for 3D-tissues. Concentration responses were investigated after acute (72 h) exposure. Moreover, the compound-specific effect of rotenone was investigated by a panel of ER-stress assay, TUNEL assay, immunocytochemistry, electron microscopy, and in 3D-spheroid based neurite outgrowth assay. The acute exposure to different classes of toxicants revealed distinct susceptibility profiles in a differentiation stage-dependent manner, indicating that hiPSC-based 3D in vitro neurosphere models could be used effectively to evaluate NT, and can be developed further to detect developmental neurotoxicity (DNT) and thus replace or complement the use of animal models in various basic research and pharmaceutical applications. induced pluripotent stem cells neurospheres 3D culture neurite outgrowth neurotoxicity Cytology Annamaria Teglasi verfasserin aut Tamas Bellak verfasserin aut Zofia Janstova verfasserin aut Kinga Molnar verfasserin aut Melinda Zana verfasserin aut Istvan Bock verfasserin aut Lajos Laszlo verfasserin aut Andras Dinnyes verfasserin aut In Cells MDPI AG, 2012 9(2020), 5, p 1122 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:5, p 1122 https://doi.org/10.3390/cells9051122 kostenfrei https://doaj.org/article/f73204f0bcb143eb8ba1c965ac7f9b1f kostenfrei https://www.mdpi.com/2073-4409/9/5/1122 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 1122
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authorswithroles_txt_mv	Julianna Kobolak @@aut@@ Annamaria Teglasi @@aut@@ Tamas Bellak @@aut@@ Zofia Janstova @@aut@@ Kinga Molnar @@aut@@ Melinda Zana @@aut@@ Istvan Bock @@aut@@ Lajos Laszlo @@aut@@ Andras Dinnyes @@aut@@
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callnumber-first	Q - Science
author	Julianna Kobolak
spellingShingle	Julianna Kobolak misc QH573-671 misc induced pluripotent stem cells misc neurospheres misc 3D culture misc neurite outgrowth misc neurotoxicity misc Cytology Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening
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topic_title	QH573-671 Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening induced pluripotent stem cells neurospheres 3D culture neurite outgrowth neurotoxicity
topic	misc QH573-671 misc induced pluripotent stem cells misc neurospheres misc 3D culture misc neurite outgrowth misc neurotoxicity misc Cytology
topic_unstemmed	misc QH573-671 misc induced pluripotent stem cells misc neurospheres misc 3D culture misc neurite outgrowth misc neurotoxicity misc Cytology
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title	Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening
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title_full	Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening
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title_sort	human induced pluripotent stem cell-derived 3d-neurospheres are suitable for neurotoxicity screening
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title_auth	Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening
abstract	We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional activity of major developmental, structural, and cell-type-specific markers was investigated at weekly intervals to present the differentiation of neurons, astrocytes, and oligodendrocytes. Neurospheres were exposed to different well-known toxicants with or without neurotoxic effect (e.g., paraquat, acrylamide, or ibuprofen) and examined at various stages of the differentiation with an ATP-based cell viability assay optimized for 3D-tissues. Concentration responses were investigated after acute (72 h) exposure. Moreover, the compound-specific effect of rotenone was investigated by a panel of ER-stress assay, TUNEL assay, immunocytochemistry, electron microscopy, and in 3D-spheroid based neurite outgrowth assay. The acute exposure to different classes of toxicants revealed distinct susceptibility profiles in a differentiation stage-dependent manner, indicating that hiPSC-based 3D in vitro neurosphere models could be used effectively to evaluate NT, and can be developed further to detect developmental neurotoxicity (DNT) and thus replace or complement the use of animal models in various basic research and pharmaceutical applications.
abstractGer	We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional activity of major developmental, structural, and cell-type-specific markers was investigated at weekly intervals to present the differentiation of neurons, astrocytes, and oligodendrocytes. Neurospheres were exposed to different well-known toxicants with or without neurotoxic effect (e.g., paraquat, acrylamide, or ibuprofen) and examined at various stages of the differentiation with an ATP-based cell viability assay optimized for 3D-tissues. Concentration responses were investigated after acute (72 h) exposure. Moreover, the compound-specific effect of rotenone was investigated by a panel of ER-stress assay, TUNEL assay, immunocytochemistry, electron microscopy, and in 3D-spheroid based neurite outgrowth assay. The acute exposure to different classes of toxicants revealed distinct susceptibility profiles in a differentiation stage-dependent manner, indicating that hiPSC-based 3D in vitro neurosphere models could be used effectively to evaluate NT, and can be developed further to detect developmental neurotoxicity (DNT) and thus replace or complement the use of animal models in various basic research and pharmaceutical applications.
abstract_unstemmed	We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional activity of major developmental, structural, and cell-type-specific markers was investigated at weekly intervals to present the differentiation of neurons, astrocytes, and oligodendrocytes. Neurospheres were exposed to different well-known toxicants with or without neurotoxic effect (e.g., paraquat, acrylamide, or ibuprofen) and examined at various stages of the differentiation with an ATP-based cell viability assay optimized for 3D-tissues. Concentration responses were investigated after acute (72 h) exposure. Moreover, the compound-specific effect of rotenone was investigated by a panel of ER-stress assay, TUNEL assay, immunocytochemistry, electron microscopy, and in 3D-spheroid based neurite outgrowth assay. The acute exposure to different classes of toxicants revealed distinct susceptibility profiles in a differentiation stage-dependent manner, indicating that hiPSC-based 3D in vitro neurosphere models could be used effectively to evaluate NT, and can be developed further to detect developmental neurotoxicity (DNT) and thus replace or complement the use of animal models in various basic research and pharmaceutical applications.
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title_short	Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening
url	https://doi.org/10.3390/cells9051122 https://doaj.org/article/f73204f0bcb143eb8ba1c965ac7f9b1f https://www.mdpi.com/2073-4409/9/5/1122 https://doaj.org/toc/2073-4409
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author2	Annamaria Teglasi Tamas Bellak Zofia Janstova Kinga Molnar Melinda Zana Istvan Bock Lajos Laszlo Andras Dinnyes
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up_date	2024-07-03T23:53:41.946Z
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Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening

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