Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA

Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacr...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Varvara Chrysostomou [verfasserIn] Hector Katifelis [verfasserIn] Maria Gazouli [verfasserIn] Konstantinos Dimas [verfasserIn] Costas Demetzos [verfasserIn] Stergios Pispas [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	non-viral vectors gene delivery nucleic acids DNA random copolymers polyplexes

Übergeordnetes Werk:	In: Materials - MDPI AG, 2009, 15(2022), 7, p 2650
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:7, p 2650

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ma15072650

Katalog-ID:	DOAJ024787477

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language	English
source	In Materials 15(2022), 7, p 2650 volume:15 year:2022 number:7, p 2650
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topic_facet	non-viral vectors gene delivery nucleic acids DNA random copolymers polyplexes Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics
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callnumber-first	T - Technology
author	Varvara Chrysostomou
spellingShingle	Varvara Chrysostomou misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc non-viral vectors misc gene delivery misc nucleic acids misc DNA misc random copolymers misc polyplexes misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA
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topic_title	TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA non-viral vectors gene delivery nucleic acids DNA random copolymers polyplexes
topic	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc non-viral vectors misc gene delivery misc nucleic acids misc DNA misc random copolymers misc polyplexes misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
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title_sort	hydrophilic random cationic copolymers as polyplex-formation vectors for dna
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title_auth	Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA
abstract	Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.
abstractGer	Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.
abstract_unstemmed	Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.
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container_issue	7, p 2650
title_short	Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA
url	https://doi.org/10.3390/ma15072650 https://doaj.org/article/fd403b7f1f9f4f8ea422dad1c4c16864 https://www.mdpi.com/1996-1944/15/7/2650 https://doaj.org/toc/1996-1944
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author2	Hector Katifelis Maria Gazouli Konstantinos Dimas Costas Demetzos Stergios Pispas
author2Str	Hector Katifelis Maria Gazouli Konstantinos Dimas Costas Demetzos Stergios Pispas
ppnlink	595712649
callnumber-subject	TK - Electrical and Nuclear Engineering
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doi_str	10.3390/ma15072650
callnumber-a	TK1-9971
up_date	2024-07-04T00:23:02.503Z
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