Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis

Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR pan...
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Autor*in:	Moniot Maxime [verfasserIn] Nourrisson Céline [verfasserIn] Bonnin Virginie [verfasserIn] Damiani Céline [verfasserIn] Argy Nicolas [verfasserIn] Bonhomme Julie [verfasserIn] Fréalle Emilie [verfasserIn] Angebault Cécile [verfasserIn] Debourgogne Anne [verfasserIn] Sitterlé Emilie [verfasserIn] Flori Pierre [verfasserIn] Brunet Julie [verfasserIn] Dalle Frédéric [verfasserIn] Favennec Loïc [verfasserIn] Poirier Philippe [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis

Übergeordnetes Werk:	In: Parasite - EDP Sciences, 2015, 29, p 55(2022)
Übergeordnetes Werk:	volume:29, p 55 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1051/parasite/2022055

Katalog-ID:	DOAJ025439405

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allfields	10.1051/parasite/2022055 doi (DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6 DE-627 ger DE-627 rakwb eng RC109-216 Moniot Maxime verfasserin aut Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections. microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases Nourrisson Céline verfasserin aut Bonnin Virginie verfasserin aut Damiani Céline verfasserin aut Argy Nicolas verfasserin aut Bonhomme Julie verfasserin aut Fréalle Emilie verfasserin aut Angebault Cécile verfasserin aut Debourgogne Anne verfasserin aut Sitterlé Emilie verfasserin aut Flori Pierre verfasserin aut Brunet Julie verfasserin aut Dalle Frédéric verfasserin aut Favennec Loïc verfasserin aut Poirier Philippe verfasserin aut In Parasite EDP Sciences, 2015 29, p 55(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 55 year:2022 https://doi.org/10.1051/parasite/2022055 kostenfrei https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 55 2022
spelling	10.1051/parasite/2022055 doi (DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6 DE-627 ger DE-627 rakwb eng RC109-216 Moniot Maxime verfasserin aut Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections. microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases Nourrisson Céline verfasserin aut Bonnin Virginie verfasserin aut Damiani Céline verfasserin aut Argy Nicolas verfasserin aut Bonhomme Julie verfasserin aut Fréalle Emilie verfasserin aut Angebault Cécile verfasserin aut Debourgogne Anne verfasserin aut Sitterlé Emilie verfasserin aut Flori Pierre verfasserin aut Brunet Julie verfasserin aut Dalle Frédéric verfasserin aut Favennec Loïc verfasserin aut Poirier Philippe verfasserin aut In Parasite EDP Sciences, 2015 29, p 55(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 55 year:2022 https://doi.org/10.1051/parasite/2022055 kostenfrei https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 55 2022
allfields_unstemmed	10.1051/parasite/2022055 doi (DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6 DE-627 ger DE-627 rakwb eng RC109-216 Moniot Maxime verfasserin aut Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections. microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases Nourrisson Céline verfasserin aut Bonnin Virginie verfasserin aut Damiani Céline verfasserin aut Argy Nicolas verfasserin aut Bonhomme Julie verfasserin aut Fréalle Emilie verfasserin aut Angebault Cécile verfasserin aut Debourgogne Anne verfasserin aut Sitterlé Emilie verfasserin aut Flori Pierre verfasserin aut Brunet Julie verfasserin aut Dalle Frédéric verfasserin aut Favennec Loïc verfasserin aut Poirier Philippe verfasserin aut In Parasite EDP Sciences, 2015 29, p 55(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 55 year:2022 https://doi.org/10.1051/parasite/2022055 kostenfrei https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 55 2022
allfieldsGer	10.1051/parasite/2022055 doi (DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6 DE-627 ger DE-627 rakwb eng RC109-216 Moniot Maxime verfasserin aut Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections. microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases Nourrisson Céline verfasserin aut Bonnin Virginie verfasserin aut Damiani Céline verfasserin aut Argy Nicolas verfasserin aut Bonhomme Julie verfasserin aut Fréalle Emilie verfasserin aut Angebault Cécile verfasserin aut Debourgogne Anne verfasserin aut Sitterlé Emilie verfasserin aut Flori Pierre verfasserin aut Brunet Julie verfasserin aut Dalle Frédéric verfasserin aut Favennec Loïc verfasserin aut Poirier Philippe verfasserin aut In Parasite EDP Sciences, 2015 29, p 55(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 55 year:2022 https://doi.org/10.1051/parasite/2022055 kostenfrei https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 55 2022
allfieldsSound	10.1051/parasite/2022055 doi (DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6 DE-627 ger DE-627 rakwb eng RC109-216 Moniot Maxime verfasserin aut Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections. microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases Nourrisson Céline verfasserin aut Bonnin Virginie verfasserin aut Damiani Céline verfasserin aut Argy Nicolas verfasserin aut Bonhomme Julie verfasserin aut Fréalle Emilie verfasserin aut Angebault Cécile verfasserin aut Debourgogne Anne verfasserin aut Sitterlé Emilie verfasserin aut Flori Pierre verfasserin aut Brunet Julie verfasserin aut Dalle Frédéric verfasserin aut Favennec Loïc verfasserin aut Poirier Philippe verfasserin aut In Parasite EDP Sciences, 2015 29, p 55(2022) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:29, p 55 year:2022 https://doi.org/10.1051/parasite/2022055 kostenfrei https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 29, p 55 2022
language	English
source	In Parasite 29, p 55(2022) volume:29, p 55 year:2022
sourceStr	In Parasite 29, p 55(2022) volume:29, p 55 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Parasite
authorswithroles_txt_mv	Moniot Maxime @@aut@@ Nourrisson Céline @@aut@@ Bonnin Virginie @@aut@@ Damiani Céline @@aut@@ Argy Nicolas @@aut@@ Bonhomme Julie @@aut@@ Fréalle Emilie @@aut@@ Angebault Cécile @@aut@@ Debourgogne Anne @@aut@@ Sitterlé Emilie @@aut@@ Flori Pierre @@aut@@ Brunet Julie @@aut@@ Dalle Frédéric @@aut@@ Favennec Loïc @@aut@@ Poirier Philippe @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	527642398
id	DOAJ025439405
language_de	englisch
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callnumber-first	R - Medicine
author	Moniot Maxime
spellingShingle	Moniot Maxime misc RC109-216 misc microsporidiosis misc real-time pcr misc bio-evolution misc enterocytozoon bieneusi misc encephalitozoon intestinalis misc Infectious and parasitic diseases Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis
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topic_title	RC109-216 Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis microsporidiosis real-time pcr bio-evolution enterocytozoon bieneusi encephalitozoon intestinalis
topic	misc RC109-216 misc microsporidiosis misc real-time pcr misc bio-evolution misc enterocytozoon bieneusi misc encephalitozoon intestinalis misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc microsporidiosis misc real-time pcr misc bio-evolution misc enterocytozoon bieneusi misc encephalitozoon intestinalis misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc microsporidiosis misc real-time pcr misc bio-evolution misc enterocytozoon bieneusi misc encephalitozoon intestinalis misc Infectious and parasitic diseases
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title	Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis
ctrlnum	(DE-627)DOAJ025439405 (DE-599)DOAJebbf318b725149e4bba48777efe72dd6
title_full	Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis
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author_browse	Moniot Maxime Nourrisson Céline Bonnin Virginie Damiani Céline Argy Nicolas Bonhomme Julie Fréalle Emilie Angebault Cécile Debourgogne Anne Sitterlé Emilie Flori Pierre Brunet Julie Dalle Frédéric Favennec Loïc Poirier Philippe
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title_sort	evaluation of the bio-evolution microsporidia generic and typing real-time pcr assays for the diagnosis of intestinal microsporidiosis
callnumber	RC109-216
title_auth	Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis
abstract	Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections.
abstractGer	Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections.
abstract_unstemmed	Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples for Enterocytozoon bieneusi (n = 34, with various genotypes), Encephalitozoon intestinalis (n = 4), Encephalitozoon hellem (n = 4), and Encephalitozoon cuniculi (n = 2). We also studied a four-year survey of an inter-laboratory quality control program including 9 centers that used this commercial assay. Sensitivity and specificity of the microsporidia generic assay were 86.4% and 93.3%, respectively. Encephalitozoon hellem and Encephalitozoon cuniculi were detected by the microsporidia generic PCR assay but not by the microsporidia typing PCR assay. These results were consistent with the results of the inter-laboratory quality control program. In conclusion, Bio-Evolution Real-time PCR assays are useful tools for intestinal microsporidiosis, but negative results for microsporidia typing assays require supplementary analyses to confirm E. hellem or E. cuniculi infections.
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title_short	Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis
url	https://doi.org/10.1051/parasite/2022055 https://doaj.org/article/ebbf318b725149e4bba48777efe72dd6 https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220076/parasite220076.html https://doaj.org/toc/1776-1042
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