<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways

<i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To...
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Gespeichert in:

Autor*in:	Tsu-Ni Ping [verfasserIn] Shu-Ling Hsieh [verfasserIn] Jyh-Jye Wang [verfasserIn] Jin-Bor Chen [verfasserIn] Chih-Chung Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	bone morphogenetic protein-2 vascular calcification

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 11(2022), 23, p 3265
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:23, p 3265

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants11233265

Katalog-ID:	DOAJ025522094

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allfields	10.3390/plants11233265 doi (DE-627)DOAJ025522094 (DE-599)DOAJ3fedf3faf3ff4921a645b1dae83508cc DE-627 ger DE-627 rakwb eng QK1-989 Tsu-Ni Ping verfasserin aut <i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways. <i<Panax notoginseng</i< water extracts bone morphogenetic protein-2 vascular calcification Botany Shu-Ling Hsieh verfasserin aut Jyh-Jye Wang verfasserin aut Jin-Bor Chen verfasserin aut Chih-Chung Wu verfasserin aut In Plants MDPI AG, 2013 11(2022), 23, p 3265 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:23, p 3265 https://doi.org/10.3390/plants11233265 kostenfrei https://doaj.org/article/3fedf3faf3ff4921a645b1dae83508cc kostenfrei https://www.mdpi.com/2223-7747/11/23/3265 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 23, p 3265
spelling	10.3390/plants11233265 doi (DE-627)DOAJ025522094 (DE-599)DOAJ3fedf3faf3ff4921a645b1dae83508cc DE-627 ger DE-627 rakwb eng QK1-989 Tsu-Ni Ping verfasserin aut <i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways. <i<Panax notoginseng</i< water extracts bone morphogenetic protein-2 vascular calcification Botany Shu-Ling Hsieh verfasserin aut Jyh-Jye Wang verfasserin aut Jin-Bor Chen verfasserin aut Chih-Chung Wu verfasserin aut In Plants MDPI AG, 2013 11(2022), 23, p 3265 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:23, p 3265 https://doi.org/10.3390/plants11233265 kostenfrei https://doaj.org/article/3fedf3faf3ff4921a645b1dae83508cc kostenfrei https://www.mdpi.com/2223-7747/11/23/3265 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 23, p 3265
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allfieldsSound	10.3390/plants11233265 doi (DE-627)DOAJ025522094 (DE-599)DOAJ3fedf3faf3ff4921a645b1dae83508cc DE-627 ger DE-627 rakwb eng QK1-989 Tsu-Ni Ping verfasserin aut <i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways. <i<Panax notoginseng</i< water extracts bone morphogenetic protein-2 vascular calcification Botany Shu-Ling Hsieh verfasserin aut Jyh-Jye Wang verfasserin aut Jin-Bor Chen verfasserin aut Chih-Chung Wu verfasserin aut In Plants MDPI AG, 2013 11(2022), 23, p 3265 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:23, p 3265 https://doi.org/10.3390/plants11233265 kostenfrei https://doaj.org/article/3fedf3faf3ff4921a645b1dae83508cc kostenfrei https://www.mdpi.com/2223-7747/11/23/3265 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 23, p 3265
language	English
source	In Plants 11(2022), 23, p 3265 volume:11 year:2022 number:23, p 3265
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authorswithroles_txt_mv	Tsu-Ni Ping @@aut@@ Shu-Ling Hsieh @@aut@@ Jyh-Jye Wang @@aut@@ Jin-Bor Chen @@aut@@ Chih-Chung Wu @@aut@@
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callnumber-first	Q - Science
author	Tsu-Ni Ping
spellingShingle	Tsu-Ni Ping misc QK1-989 misc <i<Panax notoginseng</i< water extracts misc bone morphogenetic protein-2 misc vascular calcification misc Botany <i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways
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topic_title	QK1-989 <i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways <i<Panax notoginseng</i< water extracts bone morphogenetic protein-2 vascular calcification
topic	misc QK1-989 misc <i<Panax notoginseng</i< water extracts misc bone morphogenetic protein-2 misc vascular calcification misc Botany
topic_unstemmed	misc QK1-989 misc <i<Panax notoginseng</i< water extracts misc bone morphogenetic protein-2 misc vascular calcification misc Botany
topic_browse	misc QK1-989 misc <i<Panax notoginseng</i< water extracts misc bone morphogenetic protein-2 misc vascular calcification misc Botany
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title	<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways
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title_full	<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways
author_sort	Tsu-Ni Ping
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author_browse	Tsu-Ni Ping Shu-Ling Hsieh Jyh-Jye Wang Jin-Bor Chen Chih-Chung Wu
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author-letter	Tsu-Ni Ping
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title_sort	<i<panax notoginseng</i< suppresses bone morphogenetic protein-2 expression in ea.hy926 endothelial cells by inhibiting the noncanonical nf-κb and wnt/β-catenin signaling pathways
callnumber	QK1-989
title_auth	<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways
abstract	<i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways.
abstractGer	<i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways.
abstract_unstemmed	<i<Panax notoginseng</i< (PN) exerts cardiovascular-disease-protective effects, but the effect of PN on reducing vascular calcification (VC) is unknown. Under the VC process, however, endothelial bone morphogenetic protein-2 (BMP-2) signals connect endothelial and smooth muscle cells. To investigate the effects of PN water extract (PNWE) on BMP-2 expression, human EA.hy926 endothelial cells were pretreated with PNWE for 48 h, and BMP-2 expression was then induced using warfarin/β-glycerophosphate (W/BGP) for another 24 h. The expression of BMP-2, the degrees of oxidative stress and inflammation, and the activation of noncanonical NF-κB and Wnt/β-catenin signaling were analyzed. The results showed that the BMP-2 levels in EA.hy926 cells were reduced in the groups treated with 10, 50, or 100 μg/mL PNWE combined with W/BGP. PNWE combined with W/BGP significantly reduced thiobarbituric-acid-reactive substrate and reactive oxygen species levels as well as prostaglandin E2, IL-1β, IL-6, and TNF-α. PNWE (10, 50, and 100 μg/mL) reduced the p52 levels and p52/p100 protein ratio. Wnt and β-catenin protein expression was decreased in the groups treated with PNWE combined with W/BGP. These results showed that PNWE reduced BMP-2 expression in EA.hy926 cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways.
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container_issue	23, p 3265
title_short	<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways
url	https://doi.org/10.3390/plants11233265 https://doaj.org/article/3fedf3faf3ff4921a645b1dae83508cc https://www.mdpi.com/2223-7747/11/23/3265 https://doaj.org/toc/2223-7747
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up_date	2024-07-03T15:30:02.679Z
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<i<Panax notoginseng</i< Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways

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