Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)

SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solut...
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Autor*in:	Anna Aviñó [verfasserIn] Carlos Cuestas-Ayllón [verfasserIn] Manuel Gutiérrez-Capitán [verfasserIn] Lluisa Vilaplana [verfasserIn] Valeria Grazu [verfasserIn] Véronique Noé [verfasserIn] Eva Balada [verfasserIn] Antonio Baldi [verfasserIn] Alex J. Félix [verfasserIn] Eva Aubets [verfasserIn] Simonas Valiuska [verfasserIn] Arnau Domínguez [verfasserIn] Raimundo Gargallo [verfasserIn] Ramon Eritja [verfasserIn] M.-Pilar Marco [verfasserIn] César Fernández-Sánchez [verfasserIn] Jesús Martínez de la Fuente [verfasserIn] Carlos J. Ciudad [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Polypurine reverse-Hoogsteen hairpin SARS-CoV-2 RNA detection COVID diagnosis thermal lateral flow electrochemical magnetoassay fluorescent microarray

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 23, p 15258
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:23, p 15258

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms232315258

Katalog-ID:	DOAJ025550896

Internformat


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allfieldsGer	10.3390/ijms232315258 doi (DE-627)DOAJ025550896 (DE-599)DOAJ3cbe8aaccb9a41f2a1227cd9701b6cb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Anna Aviñó verfasserin aut Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence. Polypurine reverse-Hoogsteen hairpin SARS-CoV-2 RNA detection COVID diagnosis thermal lateral flow electrochemical magnetoassay fluorescent microarray Biology (General) Chemistry Carlos Cuestas-Ayllón verfasserin aut Manuel Gutiérrez-Capitán verfasserin aut Lluisa Vilaplana verfasserin aut Valeria Grazu verfasserin aut Véronique Noé verfasserin aut Eva Balada verfasserin aut Antonio Baldi verfasserin aut Alex J. Félix verfasserin aut Eva Aubets verfasserin aut Simonas Valiuska verfasserin aut Arnau Domínguez verfasserin aut Raimundo Gargallo verfasserin aut Ramon Eritja verfasserin aut M.-Pilar Marco verfasserin aut César Fernández-Sánchez verfasserin aut Jesús Martínez de la Fuente verfasserin aut Carlos J. Ciudad verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15258 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15258 https://doi.org/10.3390/ijms232315258 kostenfrei https://doaj.org/article/3cbe8aaccb9a41f2a1227cd9701b6cb3 kostenfrei https://www.mdpi.com/1422-0067/23/23/15258 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15258
allfieldsSound	10.3390/ijms232315258 doi (DE-627)DOAJ025550896 (DE-599)DOAJ3cbe8aaccb9a41f2a1227cd9701b6cb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Anna Aviñó verfasserin aut Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence. Polypurine reverse-Hoogsteen hairpin SARS-CoV-2 RNA detection COVID diagnosis thermal lateral flow electrochemical magnetoassay fluorescent microarray Biology (General) Chemistry Carlos Cuestas-Ayllón verfasserin aut Manuel Gutiérrez-Capitán verfasserin aut Lluisa Vilaplana verfasserin aut Valeria Grazu verfasserin aut Véronique Noé verfasserin aut Eva Balada verfasserin aut Antonio Baldi verfasserin aut Alex J. Félix verfasserin aut Eva Aubets verfasserin aut Simonas Valiuska verfasserin aut Arnau Domínguez verfasserin aut Raimundo Gargallo verfasserin aut Ramon Eritja verfasserin aut M.-Pilar Marco verfasserin aut César Fernández-Sánchez verfasserin aut Jesús Martínez de la Fuente verfasserin aut Carlos J. Ciudad verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15258 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15258 https://doi.org/10.3390/ijms232315258 kostenfrei https://doaj.org/article/3cbe8aaccb9a41f2a1227cd9701b6cb3 kostenfrei https://www.mdpi.com/1422-0067/23/23/15258 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15258
language	English
source	In International Journal of Molecular Sciences 23(2022), 23, p 15258 volume:23 year:2022 number:23, p 15258
sourceStr	In International Journal of Molecular Sciences 23(2022), 23, p 15258 volume:23 year:2022 number:23, p 15258
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Polypurine reverse-Hoogsteen hairpin SARS-CoV-2 RNA detection COVID diagnosis thermal lateral flow electrochemical magnetoassay fluorescent microarray Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Anna Aviñó @@aut@@ Carlos Cuestas-Ayllón @@aut@@ Manuel Gutiérrez-Capitán @@aut@@ Lluisa Vilaplana @@aut@@ Valeria Grazu @@aut@@ Véronique Noé @@aut@@ Eva Balada @@aut@@ Antonio Baldi @@aut@@ Alex J. Félix @@aut@@ Eva Aubets @@aut@@ Simonas Valiuska @@aut@@ Arnau Domínguez @@aut@@ Raimundo Gargallo @@aut@@ Ramon Eritja @@aut@@ M.-Pilar Marco @@aut@@ César Fernández-Sánchez @@aut@@ Jesús Martínez de la Fuente @@aut@@ Carlos J. Ciudad @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ025550896
language_de	englisch
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callnumber-first	Q - Science
author	Anna Aviñó
spellingShingle	Anna Aviñó misc QH301-705.5 misc QD1-999 misc Polypurine reverse-Hoogsteen hairpin misc SARS-CoV-2 RNA detection misc COVID diagnosis misc thermal lateral flow misc electrochemical magnetoassay misc fluorescent microarray misc Biology (General) misc Chemistry Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
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topic_title	QH301-705.5 QD1-999 Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA) Polypurine reverse-Hoogsteen hairpin SARS-CoV-2 RNA detection COVID diagnosis thermal lateral flow electrochemical magnetoassay fluorescent microarray
topic	misc QH301-705.5 misc QD1-999 misc Polypurine reverse-Hoogsteen hairpin misc SARS-CoV-2 RNA detection misc COVID diagnosis misc thermal lateral flow misc electrochemical magnetoassay misc fluorescent microarray misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc Polypurine reverse-Hoogsteen hairpin misc SARS-CoV-2 RNA detection misc COVID diagnosis misc thermal lateral flow misc electrochemical magnetoassay misc fluorescent microarray misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc Polypurine reverse-Hoogsteen hairpin misc SARS-CoV-2 RNA detection misc COVID diagnosis misc thermal lateral flow misc electrochemical magnetoassay misc fluorescent microarray misc Biology (General) misc Chemistry
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title	Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
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title_full	Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
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author_browse	Anna Aviñó Carlos Cuestas-Ayllón Manuel Gutiérrez-Capitán Lluisa Vilaplana Valeria Grazu Véronique Noé Eva Balada Antonio Baldi Alex J. Félix Eva Aubets Simonas Valiuska Arnau Domínguez Raimundo Gargallo Ramon Eritja M.-Pilar Marco César Fernández-Sánchez Jesús Martínez de la Fuente Carlos J. Ciudad
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title_sort	detection of sars-cov-2 virus by triplex enhanced nucleic acid detection assay (tenada)
callnumber	QH301-705.5
title_auth	Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
abstract	SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence.
abstractGer	SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence.
abstract_unstemmed	SARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence.
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title_short	Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
url	https://doi.org/10.3390/ijms232315258 https://doaj.org/article/3cbe8aaccb9a41f2a1227cd9701b6cb3 https://www.mdpi.com/1422-0067/23/23/15258 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Carlos Cuestas-Ayllón Manuel Gutiérrez-Capitán Lluisa Vilaplana Valeria Grazu Véronique Noé Eva Balada Antonio Baldi Alex J. Félix Eva Aubets Simonas Valiuska Arnau Domínguez Raimundo Gargallo Ramon Eritja M.-Pilar Marco César Fernández-Sánchez Jesús Martínez de la Fuente Carlos J. Ciudad
author2Str	Carlos Cuestas-Ayllón Manuel Gutiérrez-Capitán Lluisa Vilaplana Valeria Grazu Véronique Noé Eva Balada Antonio Baldi Alex J. Félix Eva Aubets Simonas Valiuska Arnau Domínguez Raimundo Gargallo Ramon Eritja M.-Pilar Marco César Fernández-Sánchez Jesús Martínez de la Fuente Carlos J. Ciudad
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up_date	2024-07-03T15:40:58.722Z
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