Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females
The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs...
Ausführliche Beschreibung
Autor*in: |
Chatsirin Nakharuthai [verfasserIn] Somkiat Sreebun [verfasserIn] Apinat Kabpha [verfasserIn] Tran Vinh Phuong [verfasserIn] Surintorn Boonanuntanasarn [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2022 |
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Übergeordnetes Werk: |
In: Animals - MDPI AG, 2011, 12(2022), 23, p 3415 |
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Übergeordnetes Werk: |
volume:12 ; year:2022 ; number:23, p 3415 |
Links: |
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DOI / URN: |
10.3390/ani12233415 |
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Katalog-ID: |
DOAJ025577565 |
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245 | 1 | 0 | |a Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females |
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520 | |a The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. | ||
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10.3390/ani12233415 doi (DE-627)DOAJ025577565 (DE-599)DOAJa71276d7bb084831b41bd62b665d67a5 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Chatsirin Nakharuthai verfasserin aut Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. snakeskin gourami <i<Trichopodus pectoralis</i< <i<ddx4</i< <i<dnd</i< Veterinary medicine Zoology Somkiat Sreebun verfasserin aut Apinat Kabpha verfasserin aut Tran Vinh Phuong verfasserin aut Surintorn Boonanuntanasarn verfasserin aut In Animals MDPI AG, 2011 12(2022), 23, p 3415 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:23, p 3415 https://doi.org/10.3390/ani12233415 kostenfrei https://doaj.org/article/a71276d7bb084831b41bd62b665d67a5 kostenfrei https://www.mdpi.com/2076-2615/12/23/3415 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 23, p 3415 |
spelling |
10.3390/ani12233415 doi (DE-627)DOAJ025577565 (DE-599)DOAJa71276d7bb084831b41bd62b665d67a5 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Chatsirin Nakharuthai verfasserin aut Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. snakeskin gourami <i<Trichopodus pectoralis</i< <i<ddx4</i< <i<dnd</i< Veterinary medicine Zoology Somkiat Sreebun verfasserin aut Apinat Kabpha verfasserin aut Tran Vinh Phuong verfasserin aut Surintorn Boonanuntanasarn verfasserin aut In Animals MDPI AG, 2011 12(2022), 23, p 3415 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:23, p 3415 https://doi.org/10.3390/ani12233415 kostenfrei https://doaj.org/article/a71276d7bb084831b41bd62b665d67a5 kostenfrei https://www.mdpi.com/2076-2615/12/23/3415 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 23, p 3415 |
allfields_unstemmed |
10.3390/ani12233415 doi (DE-627)DOAJ025577565 (DE-599)DOAJa71276d7bb084831b41bd62b665d67a5 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Chatsirin Nakharuthai verfasserin aut Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. snakeskin gourami <i<Trichopodus pectoralis</i< <i<ddx4</i< <i<dnd</i< Veterinary medicine Zoology Somkiat Sreebun verfasserin aut Apinat Kabpha verfasserin aut Tran Vinh Phuong verfasserin aut Surintorn Boonanuntanasarn verfasserin aut In Animals MDPI AG, 2011 12(2022), 23, p 3415 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:23, p 3415 https://doi.org/10.3390/ani12233415 kostenfrei https://doaj.org/article/a71276d7bb084831b41bd62b665d67a5 kostenfrei https://www.mdpi.com/2076-2615/12/23/3415 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 23, p 3415 |
allfieldsGer |
10.3390/ani12233415 doi (DE-627)DOAJ025577565 (DE-599)DOAJa71276d7bb084831b41bd62b665d67a5 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Chatsirin Nakharuthai verfasserin aut Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. snakeskin gourami <i<Trichopodus pectoralis</i< <i<ddx4</i< <i<dnd</i< Veterinary medicine Zoology Somkiat Sreebun verfasserin aut Apinat Kabpha verfasserin aut Tran Vinh Phuong verfasserin aut Surintorn Boonanuntanasarn verfasserin aut In Animals MDPI AG, 2011 12(2022), 23, p 3415 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:23, p 3415 https://doi.org/10.3390/ani12233415 kostenfrei https://doaj.org/article/a71276d7bb084831b41bd62b665d67a5 kostenfrei https://www.mdpi.com/2076-2615/12/23/3415 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 23, p 3415 |
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Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females |
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Chatsirin Nakharuthai |
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Chatsirin Nakharuthai Somkiat Sreebun Apinat Kabpha Tran Vinh Phuong Surintorn Boonanuntanasarn |
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Chatsirin Nakharuthai |
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title_sort |
characterization of <i<ddx4</i< and <i<dnd</i< homologs in snakeskin gourami (<i<trichopodus pectoralis</i<) and their expression levels during larval development and in gonads of males and females |
callnumber |
SF600-1100 |
title_auth |
Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females |
abstract |
The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. |
abstractGer |
The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. |
abstract_unstemmed |
The purpose of this study was to clone and characterize <i<ddx4</i< and <i<dnd1</i< homologs in snakeskin gourami (<i<Trichopodus pectoralis</i<) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the <i<ddx4</i< family in teleosts and the <i<dnd</i< family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both <i<ddx4</i< and <i<dnd1</i< mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of <i<ddx4</i< was high during early larval development and decreased with increasing developmental age, whereas <i<dnd1</i< expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on <i<ddx4</i< and <i<dnd,</i< and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami. |
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container_issue |
23, p 3415 |
title_short |
Characterization of <i<ddx4</i< and <i<dnd</i< Homologs in Snakeskin Gourami (<i<Trichopodus pectoralis</i<) and Their Expression Levels during Larval Development and in Gonads of Males and Females |
url |
https://doi.org/10.3390/ani12233415 https://doaj.org/article/a71276d7bb084831b41bd62b665d67a5 https://www.mdpi.com/2076-2615/12/23/3415 https://doaj.org/toc/2076-2615 |
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up_date |
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