Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions

Lipid vesicles are widely used as models for cell membranes, hosts for membrane protein studies, and containers for hydrophilic molecules. The vesicle solutions in these applications are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Elizabeth G. Kelley [verfasserIn] Emily E. Blick [verfasserIn] Vivek M. Prabhu [verfasserIn] Paul D. Butler [verfasserIn] Michihiro Nagao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	lipid vesicles small angle neutron scattering (SANS) dynamic light scattering (DLS) neutron spin echo spectroscopy (NSE) structure factor hydrodynamic factor

Übergeordnetes Werk:	In: Frontiers in Physics - Frontiers Media S.A., 2014, 10(2022)
Übergeordnetes Werk:	volume:10 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fphy.2022.866024

Katalog-ID:	DOAJ025685902

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allfieldsSound	10.3389/fphy.2022.866024 doi (DE-627)DOAJ025685902 (DE-599)DOAJ0fa5afcac3e348d49ba59a27ebbaf213 DE-627 ger DE-627 rakwb eng QC1-999 Elizabeth G. Kelley verfasserin aut Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lipid vesicles are widely used as models for cell membranes, hosts for membrane protein studies, and containers for hydrophilic molecules. The vesicle solutions in these applications are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the corresponding volume fraction of vesicles is at least a factor of three times higher than the corresponding lipid volume fraction and critically depends on the vesicle radii. Here we show that these higher than may be expected vesicle volume fractions result in measurable interactions between the vesicles as well as affect the vesicle diffusion. We show that vesicle solutions prepared with lipid mass fractions, mL, as low as ≈ 0.004, which correspond to a lipid concentration of ≈ 4 mg/mL or 5 mmol/L (mM), not only have a measurable apparent structure factor (S′(q)) in small angle neutron scattering (SANS) experiments, but that this repulsive structure factor also affects the measured diffusion coefficient at small scattering vectors (q) such as those probed with dynamic light scattering (DLS). The measured diffusion coefficients are further affected by indirect solvent mediated interactions described by a hydrodynamic factor (H(q)). Accounting for the concentration-dependence of the vesicle diffusion shows that the lipid concentration dependence measured in neutron spin echo (NSE) spectroscopy is due to differences in the effective vesicle diffusion coefficients and not the membrane fluctuation dynamics. The results have practical implications for static and dynamic scattering experiments as well as provide interesting insights into the interactions between soft lipid vesicles. lipid vesicles small angle neutron scattering (SANS) dynamic light scattering (DLS) neutron spin echo spectroscopy (NSE) structure factor hydrodynamic factor Physics Emily E. Blick verfasserin aut Vivek M. Prabhu verfasserin aut Paul D. Butler verfasserin aut Paul D. Butler verfasserin aut Paul D. Butler verfasserin aut Michihiro Nagao verfasserin aut Michihiro Nagao verfasserin aut Michihiro Nagao verfasserin aut In Frontiers in Physics Frontiers Media S.A., 2014 10(2022) (DE-627)750371749 (DE-600)2721033-9 2296424X nnns volume:10 year:2022 https://doi.org/10.3389/fphy.2022.866024 kostenfrei https://doaj.org/article/0fa5afcac3e348d49ba59a27ebbaf213 kostenfrei https://www.frontiersin.org/articles/10.3389/fphy.2022.866024/full kostenfrei https://doaj.org/toc/2296-424X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
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callnumber-first	Q - Science
author	Elizabeth G. Kelley
spellingShingle	Elizabeth G. Kelley misc QC1-999 misc lipid vesicles misc small angle neutron scattering (SANS) misc dynamic light scattering (DLS) misc neutron spin echo spectroscopy (NSE) misc structure factor misc hydrodynamic factor misc Physics Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions
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topic_title	QC1-999 Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions lipid vesicles small angle neutron scattering (SANS) dynamic light scattering (DLS) neutron spin echo spectroscopy (NSE) structure factor hydrodynamic factor
topic	misc QC1-999 misc lipid vesicles misc small angle neutron scattering (SANS) misc dynamic light scattering (DLS) misc neutron spin echo spectroscopy (NSE) misc structure factor misc hydrodynamic factor misc Physics
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title	Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions
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title_full	Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions
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author_browse	Elizabeth G. Kelley Emily E. Blick Vivek M. Prabhu Paul D. Butler Michihiro Nagao
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title_sort	interactions, diffusion, and membrane fluctuations in concentrated unilamellar lipid vesicle solutions
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title_auth	Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions
abstract	Lipid vesicles are widely used as models for cell membranes, hosts for membrane protein studies, and containers for hydrophilic molecules. The vesicle solutions in these applications are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the corresponding volume fraction of vesicles is at least a factor of three times higher than the corresponding lipid volume fraction and critically depends on the vesicle radii. Here we show that these higher than may be expected vesicle volume fractions result in measurable interactions between the vesicles as well as affect the vesicle diffusion. We show that vesicle solutions prepared with lipid mass fractions, mL, as low as ≈ 0.004, which correspond to a lipid concentration of ≈ 4 mg/mL or 5 mmol/L (mM), not only have a measurable apparent structure factor (S′(q)) in small angle neutron scattering (SANS) experiments, but that this repulsive structure factor also affects the measured diffusion coefficient at small scattering vectors (q) such as those probed with dynamic light scattering (DLS). The measured diffusion coefficients are further affected by indirect solvent mediated interactions described by a hydrodynamic factor (H(q)). Accounting for the concentration-dependence of the vesicle diffusion shows that the lipid concentration dependence measured in neutron spin echo (NSE) spectroscopy is due to differences in the effective vesicle diffusion coefficients and not the membrane fluctuation dynamics. The results have practical implications for static and dynamic scattering experiments as well as provide interesting insights into the interactions between soft lipid vesicles.
abstractGer	Lipid vesicles are widely used as models for cell membranes, hosts for membrane protein studies, and containers for hydrophilic molecules. The vesicle solutions in these applications are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the corresponding volume fraction of vesicles is at least a factor of three times higher than the corresponding lipid volume fraction and critically depends on the vesicle radii. Here we show that these higher than may be expected vesicle volume fractions result in measurable interactions between the vesicles as well as affect the vesicle diffusion. We show that vesicle solutions prepared with lipid mass fractions, mL, as low as ≈ 0.004, which correspond to a lipid concentration of ≈ 4 mg/mL or 5 mmol/L (mM), not only have a measurable apparent structure factor (S′(q)) in small angle neutron scattering (SANS) experiments, but that this repulsive structure factor also affects the measured diffusion coefficient at small scattering vectors (q) such as those probed with dynamic light scattering (DLS). The measured diffusion coefficients are further affected by indirect solvent mediated interactions described by a hydrodynamic factor (H(q)). Accounting for the concentration-dependence of the vesicle diffusion shows that the lipid concentration dependence measured in neutron spin echo (NSE) spectroscopy is due to differences in the effective vesicle diffusion coefficients and not the membrane fluctuation dynamics. The results have practical implications for static and dynamic scattering experiments as well as provide interesting insights into the interactions between soft lipid vesicles.
abstract_unstemmed	Lipid vesicles are widely used as models for cell membranes, hosts for membrane protein studies, and containers for hydrophilic molecules. The vesicle solutions in these applications are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the corresponding volume fraction of vesicles is at least a factor of three times higher than the corresponding lipid volume fraction and critically depends on the vesicle radii. Here we show that these higher than may be expected vesicle volume fractions result in measurable interactions between the vesicles as well as affect the vesicle diffusion. We show that vesicle solutions prepared with lipid mass fractions, mL, as low as ≈ 0.004, which correspond to a lipid concentration of ≈ 4 mg/mL or 5 mmol/L (mM), not only have a measurable apparent structure factor (S′(q)) in small angle neutron scattering (SANS) experiments, but that this repulsive structure factor also affects the measured diffusion coefficient at small scattering vectors (q) such as those probed with dynamic light scattering (DLS). The measured diffusion coefficients are further affected by indirect solvent mediated interactions described by a hydrodynamic factor (H(q)). Accounting for the concentration-dependence of the vesicle diffusion shows that the lipid concentration dependence measured in neutron spin echo (NSE) spectroscopy is due to differences in the effective vesicle diffusion coefficients and not the membrane fluctuation dynamics. The results have practical implications for static and dynamic scattering experiments as well as provide interesting insights into the interactions between soft lipid vesicles.
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title_short	Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions
url	https://doi.org/10.3389/fphy.2022.866024 https://doaj.org/article/0fa5afcac3e348d49ba59a27ebbaf213 https://www.frontiersin.org/articles/10.3389/fphy.2022.866024/full https://doaj.org/toc/2296-424X
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Interactions, Diffusion, and Membrane Fluctuations in Concentrated Unilamellar Lipid Vesicle Solutions

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