The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance

Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factor...
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Autor*in:	Marija D. Stanišić [verfasserIn] Nikolina Popović Kokar [verfasserIn] Predrag Ristić [verfasserIn] Ana Marija Balaž [verfasserIn] Miloš Ognjanović [verfasserIn] Veljko R. Đokić [verfasserIn] Radivoje Prodanović [verfasserIn] Tamara R. Todorović [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	horseradish peroxidase isoenzymes ZIF-8 biomimetic mineralization biocomposites biocatalysts

Übergeordnetes Werk:	In: Polymers - MDPI AG, 2011, 14(2022), 22, p 4834
Übergeordnetes Werk:	volume:14 ; year:2022 ; number:22, p 4834

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/polym14224834

Katalog-ID:	DOAJ025776886

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spelling	10.3390/polym14224834 doi (DE-627)DOAJ025776886 (DE-599)DOAJb90d7474121147579125adbba02f12d3 DE-627 ger DE-627 rakwb eng QD241-441 Marija D. Stanišić verfasserin aut The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzymeZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites. horseradish peroxidase isoenzymes ZIF-8 biomimetic mineralization biocomposites biocatalysts Organic chemistry Nikolina Popović Kokar verfasserin aut Predrag Ristić verfasserin aut Ana Marija Balaž verfasserin aut Miloš Ognjanović verfasserin aut Veljko R. Đokić verfasserin aut Radivoje Prodanović verfasserin aut Tamara R. Todorović verfasserin aut In Polymers MDPI AG, 2011 14(2022), 22, p 4834 (DE-627)61409612X (DE-600)2527146-5 20734360 nnns volume:14 year:2022 number:22, p 4834 https://doi.org/10.3390/polym14224834 kostenfrei https://doaj.org/article/b90d7474121147579125adbba02f12d3 kostenfrei https://www.mdpi.com/2073-4360/14/22/4834 kostenfrei https://doaj.org/toc/2073-4360 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 22, p 4834
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allfieldsSound	10.3390/polym14224834 doi (DE-627)DOAJ025776886 (DE-599)DOAJb90d7474121147579125adbba02f12d3 DE-627 ger DE-627 rakwb eng QD241-441 Marija D. Stanišić verfasserin aut The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzymeZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites. horseradish peroxidase isoenzymes ZIF-8 biomimetic mineralization biocomposites biocatalysts Organic chemistry Nikolina Popović Kokar verfasserin aut Predrag Ristić verfasserin aut Ana Marija Balaž verfasserin aut Miloš Ognjanović verfasserin aut Veljko R. Đokić verfasserin aut Radivoje Prodanović verfasserin aut Tamara R. Todorović verfasserin aut In Polymers MDPI AG, 2011 14(2022), 22, p 4834 (DE-627)61409612X (DE-600)2527146-5 20734360 nnns volume:14 year:2022 number:22, p 4834 https://doi.org/10.3390/polym14224834 kostenfrei https://doaj.org/article/b90d7474121147579125adbba02f12d3 kostenfrei https://www.mdpi.com/2073-4360/14/22/4834 kostenfrei https://doaj.org/toc/2073-4360 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 22, p 4834
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authorswithroles_txt_mv	Marija D. Stanišić @@aut@@ Nikolina Popović Kokar @@aut@@ Predrag Ristić @@aut@@ Ana Marija Balaž @@aut@@ Miloš Ognjanović @@aut@@ Veljko R. Đokić @@aut@@ Radivoje Prodanović @@aut@@ Tamara R. Todorović @@aut@@
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callnumber-first	Q - Science
author	Marija D. Stanišić
spellingShingle	Marija D. Stanišić misc QD241-441 misc horseradish peroxidase misc isoenzymes misc ZIF-8 misc biomimetic mineralization misc biocomposites misc biocatalysts misc Organic chemistry The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
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topic_title	QD241-441 The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance horseradish peroxidase isoenzymes ZIF-8 biomimetic mineralization biocomposites biocatalysts
topic	misc QD241-441 misc horseradish peroxidase misc isoenzymes misc ZIF-8 misc biomimetic mineralization misc biocomposites misc biocatalysts misc Organic chemistry
topic_unstemmed	misc QD241-441 misc horseradish peroxidase misc isoenzymes misc ZIF-8 misc biomimetic mineralization misc biocomposites misc biocatalysts misc Organic chemistry
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title	The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
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title_full	The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
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author_browse	Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović
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title_sort	influence of isoenzyme composition and chemical modification on horseradish peroxidase@zif-8 biocomposite performance
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title_auth	The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
abstract	Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzymeZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.
abstractGer	Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzymeZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.
abstract_unstemmed	Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzymeZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.
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title_short	The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
url	https://doi.org/10.3390/polym14224834 https://doaj.org/article/b90d7474121147579125adbba02f12d3 https://www.mdpi.com/2073-4360/14/22/4834 https://doaj.org/toc/2073-4360
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up_date	2024-07-03T17:02:44.568Z
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