Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<

Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in a...
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Autor*in:	Samantha Moratal [verfasserIn] María Auxiliadora Dea-Ayuela [verfasserIn] Alba Martí-Marco [verfasserIn] Silvia Puigcercós [verfasserIn] Naima María Marco-Hirs [verfasserIn] Candela Doménech [verfasserIn] Elena Corcuera [verfasserIn] Jesús Cardells [verfasserIn] Victor Lizana [verfasserIn] Jordi López-Ramon [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	genetic characterization marine fish Mediterranean zoonotic

Übergeordnetes Werk:	In: Animals - MDPI AG, 2011, 12(2022), 9, p 1052
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:9, p 1052

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ani12091052

Katalog-ID:	DOAJ025990292

Internformat


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allfields	10.3390/ani12091052 doi (DE-627)DOAJ025990292 (DE-599)DOAJbe3027cb6a2242f09d047b334272e2a6 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Samantha Moratal verfasserin aut Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk. <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology María Auxiliadora Dea-Ayuela verfasserin aut Alba Martí-Marco verfasserin aut Silvia Puigcercós verfasserin aut Naima María Marco-Hirs verfasserin aut Candela Doménech verfasserin aut Elena Corcuera verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut Jordi López-Ramon verfasserin aut In Animals MDPI AG, 2011 12(2022), 9, p 1052 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:9, p 1052 https://doi.org/10.3390/ani12091052 kostenfrei https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 kostenfrei https://www.mdpi.com/2076-2615/12/9/1052 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 9, p 1052
spelling	10.3390/ani12091052 doi (DE-627)DOAJ025990292 (DE-599)DOAJbe3027cb6a2242f09d047b334272e2a6 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Samantha Moratal verfasserin aut Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk. <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology María Auxiliadora Dea-Ayuela verfasserin aut Alba Martí-Marco verfasserin aut Silvia Puigcercós verfasserin aut Naima María Marco-Hirs verfasserin aut Candela Doménech verfasserin aut Elena Corcuera verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut Jordi López-Ramon verfasserin aut In Animals MDPI AG, 2011 12(2022), 9, p 1052 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:9, p 1052 https://doi.org/10.3390/ani12091052 kostenfrei https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 kostenfrei https://www.mdpi.com/2076-2615/12/9/1052 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 9, p 1052
allfields_unstemmed	10.3390/ani12091052 doi (DE-627)DOAJ025990292 (DE-599)DOAJbe3027cb6a2242f09d047b334272e2a6 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Samantha Moratal verfasserin aut Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk. <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology María Auxiliadora Dea-Ayuela verfasserin aut Alba Martí-Marco verfasserin aut Silvia Puigcercós verfasserin aut Naima María Marco-Hirs verfasserin aut Candela Doménech verfasserin aut Elena Corcuera verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut Jordi López-Ramon verfasserin aut In Animals MDPI AG, 2011 12(2022), 9, p 1052 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:9, p 1052 https://doi.org/10.3390/ani12091052 kostenfrei https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 kostenfrei https://www.mdpi.com/2076-2615/12/9/1052 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 9, p 1052
allfieldsGer	10.3390/ani12091052 doi (DE-627)DOAJ025990292 (DE-599)DOAJbe3027cb6a2242f09d047b334272e2a6 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Samantha Moratal verfasserin aut Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk. <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology María Auxiliadora Dea-Ayuela verfasserin aut Alba Martí-Marco verfasserin aut Silvia Puigcercós verfasserin aut Naima María Marco-Hirs verfasserin aut Candela Doménech verfasserin aut Elena Corcuera verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut Jordi López-Ramon verfasserin aut In Animals MDPI AG, 2011 12(2022), 9, p 1052 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:9, p 1052 https://doi.org/10.3390/ani12091052 kostenfrei https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 kostenfrei https://www.mdpi.com/2076-2615/12/9/1052 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 9, p 1052
allfieldsSound	10.3390/ani12091052 doi (DE-627)DOAJ025990292 (DE-599)DOAJbe3027cb6a2242f09d047b334272e2a6 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Samantha Moratal verfasserin aut Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk. <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology María Auxiliadora Dea-Ayuela verfasserin aut Alba Martí-Marco verfasserin aut Silvia Puigcercós verfasserin aut Naima María Marco-Hirs verfasserin aut Candela Doménech verfasserin aut Elena Corcuera verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut Jordi López-Ramon verfasserin aut In Animals MDPI AG, 2011 12(2022), 9, p 1052 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:12 year:2022 number:9, p 1052 https://doi.org/10.3390/ani12091052 kostenfrei https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 kostenfrei https://www.mdpi.com/2076-2615/12/9/1052 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 9, p 1052
language	English
source	In Animals 12(2022), 9, p 1052 volume:12 year:2022 number:9, p 1052
sourceStr	In Animals 12(2022), 9, p 1052 volume:12 year:2022 number:9, p 1052
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic Veterinary medicine Zoology
isfreeaccess_bool	true
container_title	Animals
authorswithroles_txt_mv	Samantha Moratal @@aut@@ María Auxiliadora Dea-Ayuela @@aut@@ Alba Martí-Marco @@aut@@ Silvia Puigcercós @@aut@@ Naima María Marco-Hirs @@aut@@ Candela Doménech @@aut@@ Elena Corcuera @@aut@@ Jesús Cardells @@aut@@ Victor Lizana @@aut@@ Jordi López-Ramon @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	657589306
id	DOAJ025990292
language_de	englisch
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callnumber-first	S - Agriculture
author	Samantha Moratal
spellingShingle	Samantha Moratal misc SF600-1100 misc QL1-991 misc <i<Cryptosporidium</i< misc genetic characterization misc marine fish misc Mediterranean misc zoonotic misc Veterinary medicine misc Zoology Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<
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topic_title	SF600-1100 QL1-991 Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i< <i<Cryptosporidium</i< genetic characterization marine fish Mediterranean zoonotic
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title	Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<
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title_full	Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<
author_sort	Samantha Moratal
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author_browse	Samantha Moratal María Auxiliadora Dea-Ayuela Alba Martí-Marco Silvia Puigcercós Naima María Marco-Hirs Candela Doménech Elena Corcuera Jesús Cardells Victor Lizana Jordi López-Ramon
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title_sort	molecular characterization of <i<cryptosporidium</i< spp. in cultivated and wild marine fishes from western mediterranean with the first detection of zoonotic <i<cryptosporidium ubiquitum</i<
callnumber	SF600-1100
title_auth	Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<
abstract	Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk.
abstractGer	Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk.
abstract_unstemmed	Fish not only harbor host-specific species/genotypes of <i<Cryptosporidium</i<, but also species like zoonotic <i<C. parvum</i< or anthroponotic <i<C. hominis</i<, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). <i<C. molnari</i< was identified in thirteen specimens from seven different host species. Zoonotic <i<C. ubiquitum</i< was detected in two European sea bass (<i<Dicentrarchus labrax</i<). One isolate similar to <i<C. scophthalmi</i< was detected in a cultivated meagre (<i<Argyrosomus regius</i<), and one isolate, highly divergent from all the <i<Cryptosporidium</i< species/genotypes described, was identified from a synanthropic round sardinella (<i<Sardinella aurita</i<). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for <i<C. molnari</i< and identifying, for the first time, zoonotic <i<C. ubiquitum</i< in edible marine fishes, pointing out a potential health risk.
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title_short	Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<
url	https://doi.org/10.3390/ani12091052 https://doaj.org/article/be3027cb6a2242f09d047b334272e2a6 https://www.mdpi.com/2076-2615/12/9/1052 https://doaj.org/toc/2076-2615
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Molecular Characterization of <i<Cryptosporidium</i< spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic <i<Cryptosporidium ubiquitum</i<

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