Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand

Plant species confirmation is a crucial step in using native plant species for forest restoration. To enhance this, a hybrid method of DNA barcoding and high-resolution melting analysis (Bar-HRM) was investigated in this study. In total, 12 native plant species samples were collected from forest res...
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Autor*in:	Maslin Osathanunkul [verfasserIn] Nipitpong Sawongta [verfasserIn] Panagiotis Madesis [verfasserIn] Wittaya Pheera [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	native species tropical forest high-resolution melting analysis species confirmation

Übergeordnetes Werk:	In: Forests - MDPI AG, 2010, 13(2022), 7, p 997
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:7, p 997

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/f13070997

Katalog-ID:	DOAJ026223627

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callnumber-first	Q - Science
author	Maslin Osathanunkul
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topic_title	QK900-989 Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand native species tropical forest high-resolution melting analysis species confirmation
topic	misc QK900-989 misc native species misc tropical forest misc high-resolution melting analysis misc species confirmation misc Plant ecology
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title	Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand
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title_auth	Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand
abstract	Plant species confirmation is a crucial step in using native plant species for forest restoration. To enhance this, a hybrid method of DNA barcoding and high-resolution melting analysis (Bar-HRM) was investigated in this study. In total, 12 native plant species samples were collected from forest restoration sites in Nan, a province in Northern Thailand. Simulation HRM analysis was performed to find the most appropriate region for in vitro Bar-HRM analysis. After that, in vitro Bar-HRM was carried out to validate the performance of native plant species. Results from both simulation and in vitro analyses revealed that the nuclear ribosomal internal transcribed spacer (ITS) region can be used as a primer set that can clearly discriminate native plant species in this study. With our study, Bar-HRM was proved of use in native plant species confirmation, even if that species had no molecular data available. In this context, Bar-HRM would be useful for the identification of native plant species used in tropical forest restoration not only in Thailand but also in any areas with similar plant groups.
abstractGer	Plant species confirmation is a crucial step in using native plant species for forest restoration. To enhance this, a hybrid method of DNA barcoding and high-resolution melting analysis (Bar-HRM) was investigated in this study. In total, 12 native plant species samples were collected from forest restoration sites in Nan, a province in Northern Thailand. Simulation HRM analysis was performed to find the most appropriate region for in vitro Bar-HRM analysis. After that, in vitro Bar-HRM was carried out to validate the performance of native plant species. Results from both simulation and in vitro analyses revealed that the nuclear ribosomal internal transcribed spacer (ITS) region can be used as a primer set that can clearly discriminate native plant species in this study. With our study, Bar-HRM was proved of use in native plant species confirmation, even if that species had no molecular data available. In this context, Bar-HRM would be useful for the identification of native plant species used in tropical forest restoration not only in Thailand but also in any areas with similar plant groups.
abstract_unstemmed	Plant species confirmation is a crucial step in using native plant species for forest restoration. To enhance this, a hybrid method of DNA barcoding and high-resolution melting analysis (Bar-HRM) was investigated in this study. In total, 12 native plant species samples were collected from forest restoration sites in Nan, a province in Northern Thailand. Simulation HRM analysis was performed to find the most appropriate region for in vitro Bar-HRM analysis. After that, in vitro Bar-HRM was carried out to validate the performance of native plant species. Results from both simulation and in vitro analyses revealed that the nuclear ribosomal internal transcribed spacer (ITS) region can be used as a primer set that can clearly discriminate native plant species in this study. With our study, Bar-HRM was proved of use in native plant species confirmation, even if that species had no molecular data available. In this context, Bar-HRM would be useful for the identification of native plant species used in tropical forest restoration not only in Thailand but also in any areas with similar plant groups.
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title_short	Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand
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score	7.40042

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Bar-HRM for Species Confirmation of Native Plants Used in Forest Restoration in Northern Thailand

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